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Genetic relationships within Brassica rapa as inferred from AFLP fingerprints

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Abstract

Amplified fragment length polymorphism (AFLP) markers were employed to assess the genetic diversity amongst two large collections of Brassica rapa accessions. Collection A consisted of 161 B. rapa accessions representing different morphotypes among the cultivated B. rapa, including traditional and modern cultivars and breeding materials from geographical locations from all over the world and two Brassica napus accessions. Collection B consisted of 96 accessions, representing mainly leafy vegetable types cultivated in China. On the basis of the AFLP data obtained, we constructed phenetic trees using mega 2.1 software. The level of polymorphism was very high, and it was evident that the amount of genetic variation present within the groups was often comparable to the variation between the different cultivar groups. Cluster analysis revealed groups, often with low bootstrap values, which coincided with cultivar groups. The most interesting information revealed by the phenetic trees was that different morphotypes are often more related to other morphotypes from the same region (East Asia vs. Europe) than to similar morphotypes from different regions, suggesting either an independent origin and or a long and separate domestication and breeding history in both regions.

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Acknowledgements

We thank P. van der Berg for technical support and the Wageningen Plant Sciences Experimental Centre of Wageningen University for taking care of the plants. We thank Noortje Bas from the Centre for Genetic Resources The Netherlands (CGN) Wageningen-UR, Li XiXiang from the Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences and Xu Zeyong, Oil Crop Research Institute, Chinese Academy of Agricultural Sciences, for kindly supplying the accessions used in this study. This project is sponsored by the Asian Facility (project AF01/CH/8 “Sino-Dutch Genomic Lab and Vegetable Research Center”).

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Authors and Affiliations

  1. Laboratory of Genetics, Wageningen University, Arboretumlaan 4, 6703 BD, Wageningen, The Netherlands

    Jianjun Zhao & Maarten Koornneef

  2. Laboratory of Plant Physiology, Wageningen University, Arboretumlaan 4, 6703 BD, Wageningen, The Netherlands

    Jianjun Zhao

  3. Laboratory of Plant Breeding, Wageningen University, Binnenhaven 5, 6709 PD, Wageningen, The Netherlands

    Jianjun Zhao, Jaap Vromans & Guusje Bonnema

  4. Life Science College, Hebei Agricultural University, Hebei Province, Baoding, 071001, People’s Republic of China

    Jianjun Zhao

  5. Oil Crop Research Institute, Chinese Academy of Agricultural Sciences, No 2 Xudong 2nd Road, Wuhan, 430026, People’s Republic of China

    Jianjun Zhao & Zeyong Xu

  6. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie, Beijing, 100081, People’s Republic of China

    Jianjun Zhao, Xiaowu Wang, Bo Deng, Ping Lou, Jian Wu & Rifei Sun

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  1. Jianjun Zhao
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  2. Xiaowu Wang
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  3. Bo Deng
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  7. Zeyong Xu
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  8. Jaap Vromans
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  9. Maarten Koornneef
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  10. Guusje Bonnema
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Correspondence to Guusje Bonnema.

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Communicated by H.C. Becker

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Zhao, J., Wang, X., Deng, B. et al. Genetic relationships within Brassica rapa as inferred from AFLP fingerprints. Theor Appl Genet 110, 1301–1314 (2005). https://doi.org/10.1007/s00122-005-1967-y

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  • DOI: https://doi.org/10.1007/s00122-005-1967-y

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