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Mapping of a locus for adult plant resistance to downy mildew in broccoli (Brassica oleracea convar. italica)

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Abstract

The identification of the gene Pp523, conferring downy mildew resistance to adult plants of broccoli (Brassica oleracea convar. italica), led to the construction of a genetic map that included this resistance locus, 301 amplified fragment length polymorphisms, 55 random amplified polymorphic DNAs, 46 inter-simple sequence repeats, three simple sequence repeats, four other PCR markers and a flower colour locus, all gathered into nine major linkage groups. Nineteen additional molecular markers were clustered into one group of four markers, one group of three markers and six pairs of markers. The map spans over 731.9 cM, corresponding to 89.5% of the 818 cM estimated to be the total genome length. A significant number of the mapped markers, 19.3%, showed distorted segregation. The average distance between mapped adjacent markers is 1.64 cM, which places this map among the densest published to date for this species. Using bulked segregant analysis, we identified a group of molecular markers flanking and closely linked in coupling to the resistance gene and included these in the map. Two markers linked in coupling, OPK17_980 and AT.CTA_133/134, are located at 3.1 cM and 3.6 cM, respectively, at each side from the resistance gene. These markers can be used for marker-assisted selection in breeding programs aiming at the introgression of this gene in susceptible B. oleracea genotypes. The fine mapping of the genomic region surrounding the Pp523 resistance gene is currently being carried out, a basic condition for its isolation via positional cloning.

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References

  • Agnola B, Silue D, Boury S (2000) Identification of RAPD markers of downy mildew (Peronospora parasitica) resistance gene in broccoli (Brassica oleracea var. italica). In: (King GJ ) Abstr 3rd Int Symp Brassicas. Horticulture Research International, Wellesbourne, UK, poster 64

  • Bohuon EJR, Keith DJ, Parkin IAP, Sharpe AG, Lydiate DJ (1996) Alignment of the conserved C genomes of Brassica oleracea and Brassica napus. Theor Appl Genet 93:833–839

    CAS  Google Scholar 

  • Botella MA, Parker JE, Frost LN, Bittner-Eddy OD, Beynon JL, Daniels MJ, Holub EB, Jones JDG (1998) Three genes of the Arabidopsis RPP1 complex resistance locus recognize distinct Peronospora parasitica avirulence determinants. Plant Cell 10:1847–1860

    Article  CAS  PubMed  Google Scholar 

  • Cabrita LF, Aksoy U, Hepaksoy S, Leitao JM (2001) Suitability of isozyme, RAPD and AFLP markers to assess genetic differences and relatedness among fig (Ficus carica L.) clones. Sci Hortic 87:261–273

    Article  CAS  Google Scholar 

  • Cai D, Kleine M, Kifle S, Harloff HJ, Sandal NN, Marcker KA, Klein-Lankhorst RM, Salentijn EMJ, Lange W, Stiekema WJ, Wyss U, Grundler FMW, Jung C (1997) Positional cloning of nematode resistance in sugar beet. Science 275:832–834

    Article  CAS  PubMed  Google Scholar 

  • Carlier JD, Reis A, Duval MF, d’Eeckenbrugge GC, Leitão JM (2004) Genetic maps of RAPD, AFLP and ISSR markers in Ananas bracteatus and A. comosus using the pseudo-testcross strategy. Plant Breed 123:186–192

    Article  CAS  Google Scholar 

  • Chakravarti A, Lasher LA, Reefer JE (1991) A maximum likelihood method for estimating genome length using genetic linkage data. Genetics 128:175–182

    CAS  PubMed  Google Scholar 

  • Cheung WY, Champagne G, Hubert N, Landry BS (1997) Comparison of the genetic maps of Brassica napus and Brassica oleracea. Theor Appl Genet 94:569–582

    Article  CAS  Google Scholar 

  • Coelho PS, Monteiro A (2003a) Inheritance of downy mildew resistance in mature broccoli plants. Euphytica 131:65–69

    Article  Google Scholar 

  • Coelho PS, Monteiro A (2003b) Expression of resistance to downy mildew at cotyledon and adult plant stages in Brassica oleracea L. Euphytica 133:279–284

    Article  Google Scholar 

  • Coelho PS, Leckie D, Bahcevandziev K, Valério L, Astley D, Boukema I, Crute IR, Monteiro A (1998) The relationship between cotyledon and adult plant resistance to downy mildew (Peronospora parasitica) in Brassica oleracea. Acta Hortic 459:335–342

    Google Scholar 

  • Constantinescu O, Fatehi J (2002) Peronospora-like fungi (Chromista peronosporales) parasitic on Brassicaceae and related hosts. Nova Hedwigia 74:291–334

    Article  Google Scholar 

  • Dickson MH, Petzoldt R (1993) Plant age and isolate source affect expression of downy mildew resistance in broccoli. Hortic Sci 28:730–731

    Google Scholar 

  • Farinho MJ, Coelho PS, Monteiro AA, Leitão JM (2000) RAPD and AFLP markers linked to Peronospora parasitica resistance genes in broccoli. In: King (GJ) Abstr 3rd Int Symp Brassicas. Horticulture Research International, Wellesbourne, UK poster 75

  • Fidgore SS, Ferreira ME, Slocum MK, Osborn TC (1993) Association of RFLP markers with trait loci affecting clubroot resistance and morphological characters in Brassica oleracea. Euphytica 69:33–44

    Google Scholar 

  • Giovannelli JL, Farnham MW, Wang M (2002) Development of sequence characterized amplified region markers linked to downy mildew resistance in broccoli. J Amer Soc Hortic Sci 127:597–601

    CAS  Google Scholar 

  • Hoser-Krauze J, Lakowska-Ryk E, Antosik J (1995) The inheritance of resistance of some Brassica oleracea L. cultivars and lines to downy mildew, Peronospora parasitica (Pers) ex. Fr. J Appl Genet 36:27–33

    Google Scholar 

  • Hu J, Sadowski J, Osborn TC, Landry BS, Quiros CF (1998) Linkage group alignment from four independent Brassica oleracea RFLP maps. Genome 41:226–235

    Article  CAS  Google Scholar 

  • Jensen BD, Hockenhull J, Munk L (1999) Seedling and adult plant resistance to downy mildew (Peronospora parasitica) in cauliflower (Brassica oleracea convar botrytis var botrytis). Plant Pathol 48:604–612

    Article  Google Scholar 

  • Kianian SF, Quiros CF (1992) Generation of a Brassica oleracea composite RFLP map: linkage arrangements among various populations and evolutionary implications. Theor Appl Genet 84:544–554

    Google Scholar 

  • Lan TH, Paterson AH (2000) Comparative mapping of quantitative trait loci sculpting the curd of Brassica oleracea. Genetics 155:1927–1954

    CAS  PubMed  Google Scholar 

  • Lan TH, Paterson AH (2001) Comparative mapping of QTLs determining the plant size of Brassica oleracea. Theor Appl Genet 103:383–397

    Article  CAS  Google Scholar 

  • Lan TH, Delmonte TA, Reischmann KP, Hyman J, Kowalski S, McFerson J, Kresovich S, Paterson AH (2000) EST-enriched comparative map of Brassica oleracea and Arabidopsis thaliana. Genome Res 10:776–788

    Article  CAS  PubMed  Google Scholar 

  • Landry BS, Hubert N, Crete R, Chang MS, Lincoln SE, Etoh T (1992) A genetic map of Brassica oleracea based on RFLP markers detected with expressed DNA sequences and mapping resistance genes to race 2 of Plasmodiophora brassicae (Woronin). Genome 35:409–419

    CAS  Google Scholar 

  • Mahajan V, Gill HS, More TA (1995) Inheritance of downy mildew resistance in Indian cauliflower (group III). Euphytica 86:1–3

    Google Scholar 

  • Marques CM, Araujo JA, Ferreira JG, Whetten R, O’Malley DM, Liu BH, Sederoff R (1998) AFLP genetic maps of Eucalyptus globulus and E-tereticornis. Theor Appl Genet 96:727–737

    Article  CAS  Google Scholar 

  • Martin GB, Brommonschenkel SH, Chunwongse J, Frary A, Ganal MW, Spivey R, Wu T, Earle EA, Tanksley SD (1993) Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science 262:1432–1436

    CAS  PubMed  Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    CAS  PubMed  Google Scholar 

  • Morigushi K, Kimizuka-Takagi C, Ishii K, Nomura K (1999) A genetic map based on RADP, RFLP, isozyme, morphological markers and QTL analysis for clubroot resistance in Brassica oleracea. Breed Sci 49:257–265

    Google Scholar 

  • Natti JJ, Atkin JD (1960) Inheritance of downy mildew resistance in broccoli. Phytopathology 50:241

    Google Scholar 

  • Natti JJ, Dickson MH, Atkin JD (1967) Resistance of Brassica oleracea varieties to downy mildew. Phytopathology 57:144–147

    Google Scholar 

  • Saal B, Plieske J, Hu J, Quiros CF, Struss D (2001) Microsatellite markers for genome analysis in Brassica. II. Assignment of rapeseed microsatellite to the A and C genomes and genetic mapping in Brassica oleracea L. Theor Appl Genet 102:695–699

    Article  CAS  Google Scholar 

  • Sebastian RL, Howell EC, King GK, Marshall DF, Kearsey MJ (2000) An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct double haploid mapping populations. Theor Appl Genet 100:75–81

    Article  CAS  Google Scholar 

  • Smith LB, King GJ (2000) The distribution of BoCAL-a alleles in Brassica oleracea is consistent with a genetic model for curd development and domestication of the cauliflower. Mol Breed 6:603–613

    Article  Google Scholar 

  • Swiderski MR, Innes RW (2001) The Arabidopsis PBS1 resistance gene encodes a member of a novel protein kinase subfamily. Plant J 26:101–112

    Article  CAS  PubMed  Google Scholar 

  • Van Ooijen JW, Voorrips RE (2001) joinmap 3.0, Software for the calculation of genetic linkage maps. Plant Research International, Wageningen

    Google Scholar 

  • Voorrips RE, Jongerius MC, Kanne HJ (1997) Mapping of two genes for resistance to clubroot (Plasmodiophora brassicae) in a population of double haploid lines of Brassica oleracea by means of RFLP and AFLP markers. Theor Appl Genet 94:75–82

    Article  CAS  Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    CAS  PubMed  Google Scholar 

  • Williams JGK, Kubelik AR, Livak JK, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535

    CAS  PubMed  Google Scholar 

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Acknowledgements

The authors thank Dr. Graham King from HRI-Wellesboure, UK for providing the seeds of the susceptible rapid-cycling Brassica oleracea DH line GK97362 and Mrs. Luisa Valério for assistance in the greenhouse tests. This research was supported by the Fundação para a Ciência e a Tecnologia (FCT) under the projects PRAXIS/3/3.2/HORT/2144/95 and POCTI/1999/AGR/33309. Mário Farinhó was the recipient of Ph.D. fellowship GGPXXI/BD/3834/96 awarded by FCT. Diana Svetleva and José Leitão were recipient of NATO Outreach programme fellowships.

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Author notes

  1. D. Svetleva

    Present address: Agricultural University, Mendeelev Street 12, Plovdiv, 4000, Bulgaria

Authors and Affiliations

  1. FERN, CDCTPV, Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal

    M. Farinhó, J. Carlier, D. Svetleva & J. Leitão

  2. Quinta do Marquês, Estação Agronómica Nacional, 2780-505, Oeiras, Portugal

    P. Coelho

  3. Departamento de Horticultura, Instituto Superior de Agronomia, Tapada da Ajuda, 1349-018, Lisbon, Portugal

    A. Monteiro

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  1. M. Farinhó
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  2. P. Coelho
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  3. J. Carlier
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  4. D. Svetleva
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  5. A. Monteiro
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  6. J. Leitão
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Correspondence to J. Leitão.

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Communicated by C. Möllers

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Farinhó, M., Coelho, P., Carlier, J. et al. Mapping of a locus for adult plant resistance to downy mildew in broccoli (Brassica oleracea convar. italica). Theor Appl Genet 109, 1392–1398 (2004). https://doi.org/10.1007/s00122-004-1747-0

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  • DOI: https://doi.org/10.1007/s00122-004-1747-0

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