Skip to main content
SpringerLink
Log in

Immunological identification of the mouse H4 histamine receptor on spinal cord motor neurons using a novel anti-mouse H4R antibody

  • Short Communication
  • Published:
Inflammation Research Aims and scope Submit manuscript

Abstract

In this report, we describe the development of the first anti-mouse H4 receptor antibody. Utilising this new immunological probe, new evidence is provided for oligomeric mH4Rs and the presence of H4 receptors on a subpopulation of murine motor neurons. This expands the cell types where the H4 receptor is expressed in the mammalian CNS.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  1. Coruzzi G, Adami M, Guaita E, de Esch IJ, Leurs R. Antiinflammatory and antinociceptive effects of the selective histamine H4-receptor antagonists JNJ7777120 and VUF6002 in a rat model of carrageenan-induced acute inflammation. Eur J Pharmacol. 2007;563:240–4.

    Article  CAS  PubMed  Google Scholar 

  2. Dunford PJ, Williams KN, Desai PJ, Karlsson L, McQueen D, Thurmond RL. Histamine H4 receptor antagonists are superior to traditional antihistamines in the attenuation of experimental pruritus. J Allergy Clin Immunol. 2007;119:176–83.

    Article  CAS  PubMed  Google Scholar 

  3. Connelly WM, Shenton FC, Lethbridge N, Leurs R, Waldvogel HJ, Faull RL, et al. The histamine H4 receptor is functionally expressed on neurons in the mammalian CNS. Br J Pharmacol. 2009;157(1):55–63.

    Article  CAS  PubMed  Google Scholar 

  4. van Rijn RM, van Marle A, Chazot PL, Langemeijer E, Qin Y, Shenton FC, et al. Cloning and characterization of dominant negative splice variants of the human histamine H4 receptor. Biochem J. 2008;414(1):121–31.

    Article  PubMed  Google Scholar 

  5. van Rijn RM, Chazot PL, Shenton FC, Sansuk K, Bakker RA, Leurs R. Oligomerization of recombinant and endogenously expressed human histamine H(4) receptors. Mol Pharmacol. 2006;70(2):604–15.

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

The authors would like to thank the BBSRC (UK)/GSK and COST Action BM0806 for financial support. Also grateful thanks go to Prof. R Seifert (Regensburg, Germany) and colleagues at Johnson and Johnson, USA for the generous gift of cDNAs encoding mH4.

Author information

Authors and Affiliations

  1. Centre for Integrative Neuroscience, School of Biological and Biomedical Sciences, University of Durham, South Road, Durham, DH1 3LE, UK

    N. L. Lethbridge & P. L. Chazot

Authors
  1. N. L. Lethbridge
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. L. Chazot
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to P. L. Chazot.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lethbridge, N.L., Chazot, P.L. Immunological identification of the mouse H4 histamine receptor on spinal cord motor neurons using a novel anti-mouse H4R antibody. Inflamm. Res. 59 (Suppl 2), 197–198 (2010). https://doi.org/10.1007/s00011-009-0127-2

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00011-009-0127-2

Keywords

Search

Navigation