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Early administration of propofol protects against endotoxin-induced acute lung injury in rats by inhibiting the TGF-β1-Smad2 dependent pathway

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An Erratum to this article was published on 01 June 2010

Abstract

Objective

To assess the effects of propofol treatments at different time points on acute lung injury and on the expression of transforming growth factor (TGF)-β1 and the downstream target of TGF-β1, Smad 2, in the lung tissues in the endotoxic rats.

Methods

Seventy-six Wistar rats were randomly assigned to five groups: control group (saline only), endotoxemic group [lipopolysaccharide (LPS) 8 mg kg−1, i.v.], and three propofol-treated groups. For the propofol-treated groups, propofol (5 mg kg−1, i.v. bolus) was administered either 1 h before LPS, simultaneously with LPS, and 1 h after LPS, and all were followed by infusion of 10 mg kg−1 h−1 of propofol for 5 h after LPS. Lung tissues were sampled to measure myeloperoxidase activity and expression of TGF-β1 and Smad2 and to assess pulmonary microvascular permeability and histopathological changes.

Results

The hemodynamics, arterial blood gases, 5 h survival rate, pulmonary microvascular permeability, and acute lung injury scores were significantly better, and expression of TGF-β1 and Smad2 and myeloperoxidase activity in lung tissues was significantly lower in the pretreatment and simultaneous treatment groups compared to the endotoxemic group. However, there were no significant differences in all observed variables between the endotoxemic and postreatment groups. Except for TGF-β1 expression in lung tissues, the other observed variables were also not significantly different between the pretreatment and simultaneous treatment groups.

Conclusions

In the endotoxic rat model, pretreatment and simultaneous treatment with propofol provided protection against acute lung injury by inhibiting the TGF-β1-Smad2 dependent pathway.

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Acknowledgments

This project was supported in part by a grant from the Natural Science Foundation of Guangdong Province (2005-259). Other funds come from departmental sources.

Author information

Authors and Affiliations

  1. Department of Anesthesiology, Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, 111 Da De Road, 510120, Guangzhou, Guangdong, China

    Ju Gao, Wei-Xian Zhao & Shao-qun Xu

  2. Department of Anesthesiology, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China

    Fu-Shan Xue

  3. Department of Clinical Epidemiology, Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangdong, China

    Luo-Jing Zhou

  4. Department of Anesthesiology, Guangzhou First People’s Hospital, Guangzhou Medical College, Guangdong, China

    Ning Ding

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  1. Ju Gao
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  2. Wei-Xian Zhao
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Corresponding author

Correspondence to Ju Gao.

Additional information

Responsible Editor: M. Katori.

J. Gao and F.-S. Xue contributed equally to the writing of the article.

An erratum to this article can be found at http://dx.doi.org/10.1007/s00011-010-0218-0

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Gao, J., Zhao, WX., Xue, FS. et al. Early administration of propofol protects against endotoxin-induced acute lung injury in rats by inhibiting the TGF-β1-Smad2 dependent pathway. Inflamm. Res. 59, 491–500 (2010). https://doi.org/10.1007/s00011-009-0110-y

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  • DOI: https://doi.org/10.1007/s00011-009-0110-y

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