Abstract
Heavy crudes (bitumen) are extremely viscous and contain high concentrations of asphaltene, resins, nitrogen and sulfur containing heteroaromatics and several metals, particularly nickel and vanadium. These properties of heavy crude oil present serious operational problems in heavy oil production and downstream processing. There are vast deposits of heavy crude oils in many parts of the world. In fact, these reserves are estimated at more than seven times the known remaining reserves of conventional crude oils. It has been proven that reserves of conventional crude oil are being depleted, thus there is a growing interest in the utilization of these vast resources of unconventional oils to produce refined fuels and petrochemicals by upgrading. Presently, the methods used for reducing viscosity and upgradation is cost intensive, less selective and environmentally reactive. Biological processing of heavy crudes may provide an ecofriendly alternative or complementary process with less severe process conditions and higher selectivity to specific reactions to upgrade heavy crude oil. This review describes the prospects and strengths of biological processes for upgrading of heavy crude oil.
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References
Hunt, J. M. (1979)Petroleum Geochemistry and Geology. 2nd ed., W.H. Freeman, San Francisco, USA.
Martinez, A. R. (1984) Report of working group on definitions. pp. 1xvii-1xviii. In: R. F. Meyer, J. C. Wynn, and J. C. Olson (eds.),The Future of Heavy Crude and Tar Sands, Second International Conference, McGraw-Hill, New York, NY, USA.
Petersen, N. F. and P. J. Hickey (1987) California Plio-Miocene oils: Evidence of early generation. pp. 351–359. In: R. F. Meyer (eds.),Exploration for Heavy Crude Oil and Natural Bitumen.Am. Assoc. Petrol. Geol., USA.
Roadifer, R. E. (1987) Size distribution of the worlds largest known oil and tar accumulations. pp. 3–23. In: R. F. Meyer (eds.):Exploration for Heavy Crude Oil and Bitumen.Am. Assoc. Petrol. Geol., USA.
Wu, W. and J. Chen (1999) Characteristics of Chinese heavy crudes.J. Pet. Sci. Eng. 22: 25–30.
Yaghi, B. M. and A. Al-Bemani (2002) Heavy crude oil viscosity reduction for pipeline transportation.Energy Sources 24: 93–102.
Leon, V. (2000) Composition and structure of heavy oils.J. CODICID 2: 34–43.
Leon, V. (1998) New vision on heavy crude oil molecular structure.Vision Technologia 5: 131–138 (in Spanish).
Speight, J. G. (1998)The Chemistry and Technology of Petroleum. pp. 412–467. Marcel Dekker, Inc., New York, NY, USA.
Payzant, J. D., E. M. Lown, and O. P. Strausz (1991) Structural units of Athabasca asphaltene: the aromatics with a linear carbon network.Energy Fuels 5: 445–453.
Groenzin, H. and O. C. Mullins (2000) Molecular size and structure of asphaltenes from various sources.Energy Fuels 14: 677–684.
Artok, L., Y. Su, Y. Hirose, M. Hosokawa, S. Murata, and M. Nomura (1999) Structure and reactivity of petroleumderived asphaltene.Energy Fuels 13: 287–296.
Strausz, O. P., T. W. Mojelsky, E. M. Lown, I. Kowalewski, and F. Behar (1999) Structural features of Boscan and Duri asphaltenes.Energy Fuels 13: 228–247.
Strausz, O. P., T. W. Mojelsky, and E. M. Lown (1992) The molecular structure of asphaltene: an unfolding story.Fuel 71: 1355–1363.
Peng, P., A. Morales-Izquierdo, A. Hogg, and O. P. Strausz (1997) Molecular structure of athabasca asphaltene: sulfide, ether, and ester linkages.Energy Fuels 11: 1171–1187.
Bressler, D. C. and M. R. Gray (2003) Transport and reaction processes in bioremediation of organic contaminants. 1. Review of bacterial degradation and transport.Int. J. Chem. React. Eng. 1: R3.
Gray, M. R. (1994)Upgrading Petroleum Residues and Heavy Oils. Marcel Dekker, Inc., New York, NY, USA.
Pineda-Flores, G., G. Boll-Arguello, C. Lira-Galeana, and A. M. Mesta-Howard (2004) A microbial consortium isolated from a crude oil sample that uses asphaltenes as a carbon and energy source.Biodegradation 15: 145–151.
Ferrari, M. D., C. Albornoz, and E. Neirotti (1994) Biodegradability in soil of residual hydrocarbons in petroleum tank bottoms.Rev. Argent. Microbiol. 26: 157–170 (in Spanish).
Pendrys, J. P. (1989) Biodegradation of asphalt cement-20 by aerobic bacteria.Appl. Environ. Microbiol. 55: 1357–1362.
Rontani, J. F., F. Bosser-Joulak, E. Rambeloarisoa, J. C. Bertrand, and G. R. Faure (1985) Analytical study of asphalt crude oil and asphaltenes biodegradation.Chemosphere 14: 1413–1422.
Rojas-Avelizapa, N. G., E. Cervantes-Gonzalez, R. Cruz-Camarillo, and L. I. Rojas-Avelizapa (2002) Degradation of aromatic and asphaltenic fractions bySerratia liquefasciens andBacillus sp.Bull. Environ. Contam. Toxicol. 69: 833–842.
Premuzic, E. T., M. S. Lin, and B. Manowitz (1994) The significance of chemical markers in the bioprocessing of fuels.Fuel Process Technol. 40: 227–239.
Lin, M. S., E. T. Premuzic, J. H. Yablon, and W. M. Zhou (1996) Biochemical processing of heavy oils and residuum.Appl. Biochem. Biotechnol. 57/58: 659–664.
Premuzic, E. T. and M. S. Lin (1999) Induced biochemical conversions of heavy crude oils.J. Pet. Sci. Eng. 22: 171–180.
Premuzic, E. T., M. S. Lin, M. Bohenek, and W. M. Zhou (1999) Bioconversion reactions in asphaltenes and heavy crude oils.Energy Fuels 13: 297–304.
Premuzic, E. T., M. S. Lin, H. Lian, W. M. Zhou, and J. Yablon (1997) The use of chemical markers in the evaluation of crude bioconversion products, technology, and economic analysis.Fuel Process. Technol. 52: 207–223.
Premuzic, E. T., M. S. Lin, and L. Racaniello (1993) Chemical markers of induced microbial transformations in crude oils. pp. 37–54. In: E. T. Premuzic and A. Woodhead (eds.).Microbial Enhancement of Oil Recovery: Recent Advances. Elsevier, NY, USA.
Premuzic, E. T. (1994) Biochemically enhanced oil recovery and oil treatment.US patent 5,297,025.
Premuzic, E. T. and M. S. Lin (1996) Process for producing modified organisms for oil treatment at high temperatures, pressure and salinity.US Patent 5,492,828.
Premuzic, E. T. and M. S. Lin (1999) Biochemical upgrading of oils.US Patent 5,858,766.
Kanaly, A. R. and S. Harayama (2000) Biodegradation of high molecular weight polycyclic aromatic hydrocarbons by bacteria.J. Bacteriol. 182: 2059–2067.
Van Hamme, J. D., P. M. Fedorak, J. M. Foght, M. R. Gray, and H. D. Dettman (2004) Use of a novel fluorinated organosulfur compound to isolate bacteria capable of carbon-sulfur bond cleavage.Appl Environ. Microbiol. 70: 1487–1493.
Fedorak, P. M., K. M. Semple, R. Vazquez-Duhalt, and D. W. S. Westlake (1993) Chloroperoxidase-mediated modifications of petroporphyrins and asphaltenes.Enzyme Microb. Technol. 15: 429–437.
Mogollon, L., R. Rodriguez, W. Larrota, C. Ortiz, and R. Torres (1998) Biocatalytic removal of nickel and vanadium from petroporphyrins and asphaltenes.Appl. Biochem. Biotechnol. 70–72: 765–767.
Tinoco, R. and R. Vazquez-Duhalt (1998) Chemical modification of cytochromec improves their properties in oxidation of polycyclic aromatic hydrocarbons.Enzyme Microb. Technol. 22: 8–12.
Vazquez-Duhalt, R., D. W. S. Westlake, and P. M. Fedorak (1993) Cytochrome c as biocatalyst for the oxidation of thiophenes and organosulfides.Enzyme Microb. Technol. 15: 494–499.
Garcia-Arellano, H., E. Buenrostro-Gonzalez, and R. Vazquez-Duhalt (2004) Biocatalytic transformation of petroporphyrins by chemical modified cytochromec.Biotechnol. Bioeng. 85: 790–798.
Garcia-Arellano, H., B. Valderrama, G. Saab-Rincon, and R. Vazquez-Duhalt (2002) High temperature biocatalysis by chemically modified cytochrome c.Bioconjug. Chem. 13: 1336–1344.
Wernerus, H. and S. Stahl (2004) Biotechnological applications for surface-engineered bacteria.Biotechnol. Appl. Biochem. 40: 209–228.
Van Hamme, J. D., A. Singh, and O. P. Ward (2003) Recent advances in petroleum microbiology.Microbiol. Mol. Biol. Rev. 67: 503–549.
Gray, K. A., G. T. Mrachko, and C. H. Squires (2003) Biodesulfurization of fossil fuels.Curr. Opin. Microbiol. 6: 229–235.
Monticello, D. J. (2000) Biodesulfurization and the upgrading of petroleum distillates.Curr. Opin. Biotechnol. 11: 540–546.
Konishi, J., Y. Ishii, K. Okumura, and M. Suzuki (2000) High temperature desulfurization by microorganisms.US Patent 6,130,081.
Baldi, F., M. Pepi, and F. Fava (2003) Growth ofRhodosporidium toruloides strain DBVPG 6662 on dibenzothiophene crystals and orimulsion.Appl. Environ. Microbiol. 69: 4689–4696.
Bhadra, A., J. M. Scharer, and M. Moo-Young (1987) Microbial desulphurization of heavy oils and bitumen.Biotechnol. Adv. 5: 1–27.
Borgne, S. L. and R. Quintero (2003) Biotechnological processes for refining of petroleum.Fuel Process. Technol. 81: 155–169.
Benedik, M. J., P. R. Gibbs, R. R. Riddle, and R. C. Wilson (1998) Microbial denitrogenation of fossiluels.Trends Biotechnol. 16: 390–395.
Riddle, R. R., P. R. Gibbs, R. C. Wilson, and M. J. Benedik (2003) Recombinant carbazole-degrading strains for enhanced petroleum processing.J. Ind. Microbiol. Biotechnol. 30: 6–12.
Kilbane, J. J., A. Daram, J. Abbasian, and K. J. Kayser (2002) Isolation and characterization ofSphingomonas sp. GTIN11 capable of carbazole metabolism in petroleum.Biochem. Biophys. Res. Commun. 297: 242–248.
Bressler, D. C., L. A. Kirkpatrick, J. M. Foght, P. M. Fedorak, and M. R. Gray (2003) Denitrogenation of carbazole by combined biological and catalytic treatment. American Chemical Society, Petroleum Chemistry Division Preprints 48: 44–46.
Bressler, D. C., P. M. Fedorak, and M. A. Pickard (2000) Oxidation of carbazole, p-ethylcarbazole, fluorene and dibenzothiophene by the laccase ofCoriolopsis gallica.Biotechnol. Lett. 22: 1119–1125.
Xu, G. W., K. W. Mitchell, and D. J. Monticello (1998) Fuel product produced by demetalizing a fossil fuel with an enzyme.US Patent 5,624,844.
Vazquez-Duhalt, R., E. Torres, B. Valderrama, and S. Le Borgne (2002) Will biochemical catalysis impact the petroleum refining industry?Energy Fuel 16: 1239–1250.
Kirkwood, K. M., S. Ebert, D. Kharbanda, J. M. Foght, P. M. Fedorak, and M. R. Gray (2003) Bioprocessing for heavy crude oil viscosity reduction.Proceedings of the American Chemical Society. March 23–27. New Orleans, LA, USA.
Wu, Q., M. R. Gray, M. A. Pickard, P. M. Fedorak, and J. M. Foght (2003) Biocatalytic ring opening of dibenzothiophene and phenanthrene as model substrates dissolved in crude oil.Proceedings of the American Chemical Society. March 23–27, New Orleans, LA, USA.
Coyle, C. L., M. Siskin, D. T. Ferrughelli, M. S. P. Logan, and G. Zylstra (2000) Biological activation of aromatics for chemical processing and/or upgrading of aromatic compounds. petroleum coal, resid, bitumen and other petrochemical streamsUS Patent 6,136,946.
Leon, V., S. Fuenmayor, A. DeSisto, A. Marcano, S. Munoz, and A. Rivas (2003) Isolation of bacteria strains capacities in craking and desulfurization of heavy crude oil.Proceeding of 2nd ICPB The Development and Prospective of Biotechnology Applied to the Oil Industry. November 5–7. Mexico City, Mexico.
Fedorak, P. M., K. M. Semple, R. Vazquez-Duhalt, and D. W. S. Westlake (1993) Chloroperoxidasemediated modifications of petroporphyrins and asphaltenes.Enzyme Microb. Technol. 15: 429–437.
Vorbeck, C., H. Lenke, P. Fischer, and H. J. Knacknuss (1994) Identification of a hydride-Meisenheimer complex as a metabolite of 2,4,6-trinitrotoluene by aMycobacterium strain.J. Bacteriol. 176: 932–934.
Esteve-Núñez, A., A. Caballero, and J. L. Ramos (2001) Biological degradation of 2,4,6-trinitrotoluene.Microbiol. Mol. Biol. Rev. 65: 335–352.
Zhang, X., E. R. Sullivan, and L. Y. Young (2000) Evidence for aromatic ring reduction in the biodegradation pathway of carboxylated naphthalene by a sulfate reducing consortium.Biodegradation 11: 117–124.
Rieger, P.-G., V. Sinnwell, A. Preuss, W. Francke, and H.-J. Knackmuss (1999) Hydride-Meisenheimer complex formation and protonation as key reactions of 2,4,6-trinitrophenol biodegradation byRhodococcus erythropolis.J. Bacteriol. 181: 1189–1995.
Premuzic, E. T., M. S. Lin, M. Bohenek, and W. M. Zhou (1999) Bioconversion reactions in asphaltenes and heavy crude oils.Energy Fuels 13: 297–304.
Heiss, G., K. W. Hofmann, N. Trachtmann, D. M. Walters, P. Rouvière, and H.-J. Knackmuss (2002) npd gene functions ofRhodococcus erythropolis HL PM-1 in the initial steps of 2,4,6-trinitrophenol degradation.Microbiology 148: 799–806.
Miller, R. M. and R. Bartha (1989) Evidence from liposome encapsulation for transport-limited microbial metabolism of solid alkanes.Appl. Environ. Microbiol. 55: 269–274.
Kropp, K. G., I. A. Davidova, and J. M. Suflita (2000) Anaerobic oxidation of n-dodecane by an addition reaction in a sulfate-reducing bacterial enrichment culture.Appl. Environ. Microbiol. 66: 5393–5398.
Spormann, A. M. and F. Widdel (2000) Metabolism of alkylbenzenes, alkanes, and other hydrocarbons in anaerobic bacteria.Biodegradation 11: 85–105.
Widdel, F. and R. Rabus (2001) Anaerobic biodegradation of saturated and aromatic hydrocarbons.Curr. Opin. Biotechnol. 12: 259–276.
Hamer, G. and N. Al-Awadhi (2000) Biotechnological applications in the oil industry.Acta Biotechnol. 20: 335–350.
Lazar, I., A. Voicu, C. Nicolescu, D. Mucenica, S. Dobrota, I. G. Petrisor, M. Stefanescu, and L. Sandulescu (1999) The use of naturally occurring selectively isolated bacteria for inhibiting paraffin deposition.J. Pet. Sci. Eng. 22: 161–169.
Rocha, C. A., D. Gonzalez, M. L. Iturralde, U. L. Lacoa, and F. A. Morales (2000) Production of oily emulsions mediated by a microbial tenso-active agent.US Patent 6,060,287.
Iqbal, S., Z. M. Khalid, and K. A. Malik (1995) Enhanced biodegradation and emulsification of crude oil and hyperproduction of biosurfactants by a gamma ray-induced mutant ofPseudomonas aeruginosa.Lett. Appl. Microbiol. 21: 176–179.
Venkateswaran, K., T. Hoaki, M. Kato, and T. Maruyama (1995) Microbial degradation of resins fractionated from Arabian light crude oil.Can. J. Microbiol. 41: 418–424.
Barathi, S. and N. Vasudevan (2001) Utilization of petroleum hydrocarbons byPseudomonas fluorescens isolated from a petroleum-contaminated soil.Environ. Int. 26: 413–416.
Abalos, A., M. Vinas, J. Sabate, M. A. Manresa, and A. M. Solanas (2004) Enhanced biodegradation of Casablanca crude oil by a microbial consortium in presence of a rhamnolipid produced byPseudomonas aeruginosa AT10.Biodegradation 15: 249–260.
Cairns, W. L., D. G. Cooper, J. E. Zajic, J. M. Wood, and N. Kosaric (1982) Characterization ofNocardia amarea as a potent biological coalescing agent of water-oil emulsions.Appl. Environ. Microbiol. 43: 362–366.
Das, M. (2001) Characterization of de-emulsification capabilities of aMicrococcus species.Bioresour. Technol. 79: 15–22.
Nadarajah, N., A. Singh, and O. P. Ward (2002) Deemulsification of petroleum oil emulsion by a mixed bacterial culture.Process Biochem. 37: 1133–1141.
Park, S. H., J.-H. Lee, S.-H. Ko, D.-S. Lee, and H. K. Lee (2000) Demulsification of oil-in-water emulsions by aerial spores of aStreptomyces sp.Biotechnol. Lett. 22: 1389–1395.
Herman, D. C., P. M. Fedorak, M. D. MacKinnon, and J. W. Costerton (1994) Biodegradation of naphthenic acids by microbial populations indigenous to oil sands tailings.Can. J. Microbiol. 40: 467–477.
Cooper, D. G. (1982)Biosurfactants and Enhanced Oil Recovery. pp. 112–114. Proceedings of Int. Conf. Microbial Enhanced Oil Recovery, May 16–21, Afton, UK.
Bryant, R. S. and J. Douglas (1987) Evaluation of microbial systems in porous media for enhanced oil recovery, paper SPE 16284, SPE Int. Symp. on Oilfied Chemistry, Feb. 4–6, San Antonio.
Hayes, M. E., K. R. Hrebenar, P. L. Murphy, L. E. Futch, jr., J. F. Deal III, and P. L. Bolden, Jr. (1990) Bioemulsifier-stabilized hydrocarbosols.US Patent 4,943,390.
Ayala, M., R. Tinoco, V. Hernández, P. Bremuntz, and R. Vazquez-Duhalt (1998) Biocatalyticoxidation of fuel as an alternative to biodesulfurization.Fuel Process Technol. 57: 101–111.
Ayala, M., N. R. Robledo, A. Lopez-Munguia, and R. Vazquez-Duhalt (2000) Substrate specificity and ionization potential in chloroperoxidase-catalyzed oxidation of diesel fuel.Environ. Sci. Technol. 34: 2804–2809.
Huber, H. and K. O. Stetter (1998) Hyperthermophiles and their possible potential in biotechnology.J. Biotechnol. 64: 39–52.
Ward, O. P. and M. Moo-Young (1988) Thermostable enzymes.Biotechnol. Adv. 6: 39–69.
Klein, J., D. E. A. Catcheside, R. Fakoussa, L. Gazso, W. Fritsche, M. Hoefer, F. Laborda, I. Margarit, H. J. Rehm, M. Reich-Walber, W. Sand, S. Schacht, H. Schmiers, L., Setti, and A. Teinbuechel (1999) Biological processing of fuels.Appl. Microbiol. Biotechnol. 52: 2–15.
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Leon, V., Kumar, M. Biological upgrading of heavy crude oil. Biotechnol. Bioprocess Eng. 10, 471–481 (2005). https://doi.org/10.1007/BF02932281
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DOI: https://doi.org/10.1007/BF02932281