Skip to main content
SpringerLink
Log in

Merits of secretion of heterologous proteins from industrial microorganisms

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

The heterologous expression of proteins is without doubt one of the most fascinating applications of the recombinant DNA technique. Despite clear successes many attempts to produce a certain protein in a heterologous host cell have met with technical difficulties. Secretion from cells has been used as a solution to overcome the intracellular formation of inactive protein. Microorganisms with a history of use in the fermentation industry exhibit clear advantages over the frequently usedEscherichia coli as host cells for secreted products. Interleukin-3, chymosin and phytase are examples of commercial products that are produced efficiently with the aid of industrial microorganisms.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • van den Berg J.A., van der Laken K.J., van Ooyen A.J.J., Renniers T.C.H.M., Rietveld K., Schaap A.:Kluyveromyces as a host for heterologous gene expression: expression and secretion of prochymosin.Bio/Technology 8, 135–139 (1990).

    Article  PubMed  Google Scholar 

  • Danley D.E., Geoghegan K.F.: Structure and mechanism of formation of recombinant-derived chymosin C.J.Biol.Chem. 263, 9785–9789 (1988).

    PubMed  CAS  Google Scholar 

  • van Gorcom R.F.M., van Hartingsveld W., van Paridon P., Veenstra A.C., Luiten R.G.M., Selten G.C.M.: Cloning and expression of microbial phytase.PCT Pat. Appl. WO 9105053 (1991).

  • Howson S.J., Davis R.P.: Production of phytate-hydrolysing enzyme by some fungi.Enzyme Microb.Technol. 5, 377–382 (1983).

    Article  CAS  Google Scholar 

  • Johnson I.S.: Human insulin from recombinant DNA technology.Science 219, 632–637 (1983).

    Article  PubMed  CAS  Google Scholar 

  • Jongbloed W.W., Mroz Z., Kemme P.A.: The effect of supplementaryAspergillus phytase in diets for pigs.J.Anim.Sci. 70, 1159–1168 (1992).

    PubMed  CAS  Google Scholar 

  • Kricek F., Ruf C., Aschauer H., Stuchlik P., Graf C., Seifert J.M.: Hybrid genes expressing fusion peptides. A strategy for bacterial production of recombinant human interleukin-3 with a defined NH2-terminus.Ann.New York Acad.Sci. 646, 136–139 (1991).

    Article  CAS  Google Scholar 

  • van Leen R.W., Bakhuis J.G., van Beckhoven R.F.W.C., Burger H., Dorssers L.C.J., Hommes R.W.J.: Production of human interleukin-3 using industrial microorganisms.Bio/Technology 9, 47–52 (1991).

    Article  PubMed  Google Scholar 

  • Postmus P.E., Gietema A.A., Damsma O., Biesma B., Limburg P.C., Vellenga E., de Vries E.G.E.: Effects of recombinant human interleukin-3 in patients with relapsed small-cell lung cancer treated with chemotherapy: a dose-finding study.J.Clin.Oncol. 10, 1131–1140 (1992).

    PubMed  CAS  Google Scholar 

  • Quax W.J., Bonekamp A.J., van Tilborg M.: Correct secretion of heterologous proteins fromBacillus licheniformis, pp. 143–150 inIndustrial Microorganisms: Basic and Applied Molecular Genetics (Baltz, Hegeman, Skatrud, Eds). Amer. Soc. Microbiol., Washington (DC) 1993.

    Google Scholar 

  • Ward M., Wilson L.J., Kodama K.H., Rey M.W., Berka R.M.: Improved production of chymosin inAspergillus by expression as a glucoamylase-chymosin fusion.Bio/Technology 8, 435–440 (1990).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Genencor International B.V., P.O. Box 642, 2600, AP Delft, The Netherlands

    W. J. Quax

  2. Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, P.O. Box 9502, 2300, RA Leiden, The Netherlands

    W. J. Quax

Authors
  1. W. J. Quax
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Part of this study was conducted with sponsoring from theEU Commission (project CT9302540).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Quax, W.J. Merits of secretion of heterologous proteins from industrial microorganisms. Folia Microbiol 42, 99–103 (1997). https://doi.org/10.1007/BF02898715

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02898715

Keywords

Search

Navigation