Summary
Biodegradation of tricalcium phosphate (TCP) ceramics was observed through mixed culture of osteoclasts and TCP discsin vitro in this study. Osteoclasts were isolated from newborn SD rat’s marrow of long bone and cultured on TCP discs. The culture terminated at the 48th h and 96th h respectively. Under an inverted microscope, the osteoclasts imparted round or oval body with multinuclear and many thin processes. These cells were positively stained for tartrate-resistance acid phosphatase (TRAP). Scanning electron microscope showed that many resorption lacunae on TCP disc surface and their diameters were smaller than 20 μm. Osteoclasts were located in the lacunae. At the 96th h, the resorption lacunae become larger and osteoclasts showed degeneration. It is suggested that osteoclasts possess ability to re-absorb TCP ceramics underin vitro culturing condition.
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References
TCP, 1992, 12:11
1996, 13(suppl):167
Basle M F, Chappard D, Grizon Fet al. Osteoclastic resorption of Ca-P biomaterials implanted in rabbit bone. Calcif Tissue Int, 1993, 53:348
Soueidan A, Gan G I, Bouler J Met al. Biodegradation of synthetic biphasic calcium phosphate and biological calcified substratum by cells of hemopoietic origin. Cells and Materials, 1995, 5:31
De Bruijin T D, Bovell Y P, Davies J Eet al. Osteoclastic resoption of calcium phosphate is potentiated in postosteogenic culture conditions. J Biomed Mater Res, 1994, 28:105
Yamada S, Nakamura T, Kokubo Tet al. Degradation of the apatite layer formed on bioactive ceramics and the underlying ceramic surface by osteoclasts in a culture system. Cell and Mateials, 1994, 4:347
Steven L T. Bone remodeling and the osteoclast. J Bone Miner Res, 1993, 8:S523
Murrills RJ, Stein LS, Dempster DW. Stimulation of bone resorption and osteoclasts clear zone formation by low PH: a time-course study. J Cell Physios, 1993, 154:511
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This project was supported by grant from the National Natural Science Foundation of China (No. 59493200).
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Qixin, Z., Jingyuan, D., Zhidao, X. et al. Biodegradation of tricalcium phosphate ceramics by osteoclasts. Current Medical Science 18, 257–261 (1998). https://doi.org/10.1007/BF02886487
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DOI: https://doi.org/10.1007/BF02886487