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A mild, rapid, and efficient method of lipid extraction for use in determining vitamin E/lipid ratios

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Lipids

Abstract

A new, general method for lipid extraction and measurement of vitamin E/total lipid ratios in tissue and cell samples has been developed. The new extraction procedure use a combination of sodium dodecylsulfate, ethanol andn-heptane, and is mild, clean, convenient, efficient and rapid (≤5 min). The efficiency of the new method has been confirmed for human plasma, red blood cells and rat liver homogenate by the comparison of the yields of vitamin E, O-acyl lipid and cholesterol with the yields obtained following conventional extraction procedures. Extraction efficiency also has been confirmed for multilamenllar vesicles composed of known quantities of vitamin E, egg lecithin and cholesterol.

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Abbreviations

SDS:

sodium dodecyl sulfate

MLV:

multilamellar vesicles

RBC:

red blood cells

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PS:

phosphatidylserine

SP:

sphingomyelin

2-BHA:

2-t-butyl-4-hydroxyanisole

PMHC:

2,2,5,7,8-pentamethyl-6-hydroxychroman

CMC:

critical micelle concentration

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Authors and Affiliations

  1. Division of Chemistry, National Research Council of Canada, K1A OR6, Ottawa, Ontario, Canada

    Graham W. Burton, Ann Webb & Keith U. Ingold

Authors
  1. Graham W. Burton
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  2. Ann Webb
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  3. Keith U. Ingold
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N.R.C.C. No. 23769.

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Burton, G.W., Webb, A. & Ingold, K.U. A mild, rapid, and efficient method of lipid extraction for use in determining vitamin E/lipid ratios. Lipids 20, 29–39 (1985). https://doi.org/10.1007/BF02534359

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  • DOI: https://doi.org/10.1007/BF02534359

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