Abstract
Human perforin cDNA was isolated and the complete nucleotide sequence of the gene determined. The deduced amino acid sequence of human perforin showed 68.4% similarity to that of mouse perforin. RNA blot analysis of the human perforin gene revealed that the gene product is expressed preferentially in killer-type cells among cell lines tested, and in large granular lymphocytes among the peripheral blood mononuclear cells. In situ hybridization analysis with a human perforin cDNA probe revealed that the human perforin (PFP) gene is located on chromosome17q11-21.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Dourmashkin, R. R., Deteix, P., Simone, C. B., and Henkart, P.: Electron microscopic demonstration of lesions in target cell membranes associated with antibody-dependent cellular cytotoxicity.Clin Exp Immunol 42: 554–560, 1980
Ferguson, W. S., Verret, C. R., Reilly, E. B., Iannini, M. J. and Eisen, H. N.: Serme esterase and hemolytic activity in human cloned cytotoxic T lymphocytes.J Exp Med 167: 528–540, 1988
Solomon, E. and Barker, D.: Report of the committee on the genetic constitution of chromosome 17, Human Gene Mapping 9.5 (1988): Update to the Ninth International Workshop on Human Gene Mapping.Cytogenet Cell Genet 46: 95–98, 1988
Ishikawa, H., Shinkai, Y., Yagita, H., Yue, C. C., Henkart, P. A., Sawada, S., Young, H. A., Reynolds, C. W., and Okumura, K.: Molecular cloning of rat cytolysin.J Immunol, in press, 1989
Jiang, S., Persechini, P. M., Zychlinsky, A., Liu, C.-C., Perussia, B., and Young, J. D.-E.: Resistance of cytolytic lymphocytes to perforin-mediated killing: lack of correlation with complement-associated homologous species restriction.J Exp Med 168: 2207–2219, 1988
Krahenbuhl, O., Rey, C., Jenne, D., Lanzavecchia, A., Groscurth, P., Carrel, S., and Tschopp, J.: Characterization of granzymes a and b isolated from granules of cloned human cytotoxic T lymphocytes.J Immunol 141: 3471–3477, 1988
Lichtenheld, M. G., Olsen, K. J., Lu, P., Lowrey, D. M., Hameed, A., Hengartner, H., and Podack, E. R.: Structure and function of human perforin.Nature 335: 448–451, 1988
Lowrey, D. M., Aebischer, T., Olsen, K., Lichtenheld, M., Rupp, F., Hengartner, H., and Podack, E. R.: Cloning, analysis, and expression of murine perforin 1 cDNA, a component of cytolytic T-cell granules with homology to complement component C9.Proc Natl Acad Sci USA 86: 247–251, 1989
Maniatis, T., Fritsch, E. F., and Sambrook, J.:Molecular Cloning: A Laboratory Manual, p. 320, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1982
Oshimi, K., Oshimi, Y., Satake, M., and Mizoguchi, H.: Natural killermediated lysis of normal and malignant target cells, and its regulation by monocytes.J Exp Med 162: 472–486, 1985
Oshimi, K., Shinkai, Y., Okumura, K., Oshimi, Y., and Mizoguchi, H.: Perforin gene expression in granular lymphocyte proliferative disorders.Blood, in press, 1989
Rogde, S., Olaisen, B., Gedde-Dahl, T., Jr., and Teisberg, P.: The C8A and C8B loci are closely linked on chromosome 1.Ann Hum Genet 50: 139–144, 1986
Rowley, J. D., Golomb, H. M., Vardiman, J. W., Fukuhara, S., Dougherty, S., and Potter, D.: Further evidence for a consistent chromosomal abnormality in acute promyelocytic leukemia.Int J Cancer 20: 860–872, 1977
Sanger, F., Nicklen, S., and Coulson, A. R.: DNA sequencing with chain-terminating inhibitors.Proc Natl Acad Sci USA 74: 5463–5467, 1977
Shinkai, Y., Takio, K., and Okumura, K.: Homology of perforin to the ninth component of complement (C9)Nature 334: 525–527, 1988
Tschopp, J., Masson, D., and Stanley, K. K.: Structural/functional similarity between proteins involved in complement- and cytotoxic T-lymphocyte-mediated cytolysis.Nature 322: 831–834, 1986
Yanisch-Perron, C., Vieira, J., and Messing, J.: Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.Gene 33: 103–119, 1985
Yoshida, M. C., Ikeuchi, T., and Sasaki, M.: Differential staining of parental chromosomes in interspecific cell hybrids with a combined quinacrine and 33258 Hoechst techniques.Proc Japan Acad 51: 184–187, 1975
Yoshida, M. C., Satoh, H., Sasaki, M., Semba, K., Yamamoto, T., and Toyoshima, K.: Regional location of a novel yes-related protooncogene, syn, on human chromosome 6 at band q21.Jpn J Cancer Res 77: 1059–1061, 1986
Young, J. D.-E. and Cohn, Z. A.: Cellular and humoral mechanisms of cytotoxicity: structural and functional analogies.Advances in Immunology 41: 269–332, 1987
Young, J. D.-E., Cohn, Z. A., and Podack, E. R.: The ninth component of complement and the pore-forming protein (perforin) from cytotoxic T cells: structural, immunological, and functional similarities.Science 233: 184–233, 1986
Author information
Authors and Affiliations
Additional information
The nucleotide sequence data reported in this paper have been submitted to the GeBank nucleotide sequence database and have been assigned the accession number M28393.
Rights and permissions
About this article
Cite this article
Shinkai, Y., Yoshida, M.C., Maeda, K. et al. Molecular cloning and chromosomal assignment of a human perforin (PFP) gene. Immunogenetics 30, 452–457 (1989). https://doi.org/10.1007/BF02421177
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02421177