Summary
A novel approach to tailored selective excitation for the measurement of NMR spectra in non-deuterated aqueous solutions (WATERGATE, WATER suppression by GraAdient-Tailored Excitation) is described. The gradient echo sequence, which effectively combines one selective 180° radiofrequency pulse and two field gradient pulses, achieves highly selective and effective water suppression. This technique is ideally suited for the rapid collection of multi-dimensional data since a single-scan acquisition produces a pure phase NMR spectrum with a perfectly flat baseline, at the highest possible sensitivity. Application to the fast measurement of 2D NOE data of a 2.2. mM solution of a double-stranded DNA fragment in 90% H2O at 5 °C is presented.
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Davis, A.L., Keeler, J., Laue, E.D. and Moskau, D. (1992)J. Magn. Reson.,98, 207–216.
Davis, A.L., Laue, E.D., Keeler, J., Moskau, D. and Lohman, J. (1991)J. Magn. Reson.,94, 637–644.
Guéron, M., Plateau, P. and Decorps, M. (1991)Prog. NMR Spectr.,23, 135–209.
Hore, P. (1989)Methods Enzymol.,176, 64–77.
Hoult, D.I. (1976)J. Magn. Reson.,21, 337–347.
Hurd, R.E. (1990)J. Magn. Reson.,87, 422–428.
Hurd, R.E. and John, B.K. (1991)J. Magn. Reson.,91, 648–653.
Jesson, J.P., Meakin, P. and Kneissel, G. (1973)J. Am. Chem. Soc.,95, 618–620.
John, B.K., Plant, D., Webb, P. and Hurd, R.E. (1992)J. Magn. Reson.,98, 200–206.
Kumar, A., Ernst, R.R. and Wüthrich, K. (1980)Biochem. Biophys. Res. Commun.,95, 1–6.
Macura, S. and Ernst, R.R. (1980)Mol. Phys.,41, 95–117.
Marion, D., Ikura, M. and Bax, A. (1989a)J. Magn. Reson.,84, 425–430.
Marion, D., Ikura, M., Tschudin, R. and Bax, A. (1989b)J. Magn. Reson.,85, 393–399.
Otting, G. and Wüthrich, K. (1989)J. Am. Chem. Soc.,111, 1871–1875.
Otting, G., Liepinsh, E., Farmer, B.T. and Wüthrich, K. (1991)J. Biomol. NMR,1, 209–215.
Sklenář, V. and Bax, A. (1987)J. Magn. Reson.,75, 378–383.
Sklenář, V. (1990) InNMR Application in Biopolymers (Eds, Finley, J., Schmidt, S.J. and Seriani, A.S.) Academic Press, San Diego, pp. 63–84.
Vuister, G.W., Boelens, R., Kaptein, R., Hurd, R.E., John, B. and van Zilj, P.C.M. (1991)J. Am. Chem. Soc.,113, 9688–9690.
Wüthrich, K. (1986)NMR of Proteins and Nucleic Acids, Wiley, New York.
Zilj, P. and Moonen, C.T.W. (1990)J. Magn. Reson.,87, 18–25.
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Piotto, M., Saudek, V. & Sklenář, V. Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions. J Biomol NMR 2, 661–665 (1992). https://doi.org/10.1007/BF02192855
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DOI: https://doi.org/10.1007/BF02192855