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Scanning electron microscopy and epifluorescence investigation of bacterial colonization of marine sand sediments

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Abstract

Scanning electron microscopy (SEM) was employed for the investigation of microorganisms living in marine sand sediments. Epifluorescence, as well as sediment analyses, gave further data on the parameters of the sediment samples.

SEM revealed a correlation between the site and density of bacterial colonization and the microtopography of the individual sand grains.

Sand grains with a medium roundness showed the greatest density of bacterial colonization. Protected surface sites were favored in the colonization process. The mode of bacterial attachment varied; mostly the barren sand grain surface was colonized. However, bacteria were also observed close to or within detritus or attached to diatoms. Many of the attaching bacteria observed were found to produce polymer strands.

In some cases special structures were discovered which could serve bacterial attachment. Entire colonies attached by means of polymer nets, and disc-shaped bacteria were observed.

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References

  1. Harris, R. H., and R. Mitchell: The role of polymers in microbial aggregation. Annu. Rev. Microbiol.27, 27–50 (1973)

    PubMed  Google Scholar 

  2. Klainer, A. S., S. Jernigan, and P. Allender: Evaluation and comparison of techniques for examination of bacteria by scanning electron microscopy. Proc. 7th Ann. Iitri-Sem. 313–317 (1974)

  3. Meadows, P. S., and J. G. Anderson: Microorganisms attached to marine and freshwater sand grains. Nature198, 610–611 (1966)

    Google Scholar 

  4. Puleo, R., M. S. Favero, and N. J. Petersen: Use of ultrasonic energy in assessing microbial contamination on surfaces. Appl. Microbiol.15, 1345–1351 (1967)

    Google Scholar 

  5. Rheinheimer, G.: Aquatic Microbiology. John Wiley & Sons, London (1974)

    Google Scholar 

  6. Russel, R. D., and R. E. Taylor. Roundness and shape of Mississippi river sands. J. Geol.45, 225 (1937)

    Google Scholar 

  7. Santoro, T., and G. Stotzky: Sorption between microorganisms and clay minerals as determined by the sensing zone particle analyser. Can. J. Microbiol.14, 299–307 (1968)

    PubMed  Google Scholar 

  8. Schlieper, C. (Ed.): Methoden der meeresbiologischen Forschung. Fischer Verlag, Jena (1968)

    Google Scholar 

  9. Schneiderhöhn, P.: Eine vergleichende Studie über Methoden zur quantitativen Bestimmung von Abrundung und Form an Sandkörpern. Heidelberger Beitr. Miner. Petrogr.4, 172–191 (1954). In: G. Müller (1964)

    Article  Google Scholar 

  10. Seibold, E.: Der Meeresboden. Springer-Verlag, Berlin, Heidelberg, New York (1974)

    Google Scholar 

  11. Sieburth, I. McNeill, I.: Microbial Seascapes. University Park Press, Baltimore (1975)

    Google Scholar 

  12. Stevenson, I. L.: The effect of sonic vibration on the bacterial plate count of soil. Plant Soil10, 1–8 (1958)

    Article  Google Scholar 

  13. Williams, A. R., and J. S. Slade: Ultrasonic dispersal of aggregates of Sarcina lutea. Ultrasonics 4: 71, 85–87 (1971)

    Google Scholar 

  14. Williams, S. T., C. J. Veldkamp, and C. S. Robinson: Preparation of microbes for scanning electron microscopy. Proc. 6th Ann. Iitri-Sem 735–741 (1973)

  15. Zimmermann, R., and L. A. Meyer-Reil: A new method for fluorescence staining of bacterial populations on membrane filters. Kieler Meeresforsch.30, 24–27 (1974)

    Google Scholar 

  16. Zvyagintsev, D. G., and G. M. Galkina: Ultrasonic treatment as a method for preparation of soils for microbiological analysis, Mikrobiologiya36, 1087–1095 (1967)

    Google Scholar 

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Weise, W., Rheinheimer, G. Scanning electron microscopy and epifluorescence investigation of bacterial colonization of marine sand sediments. Microb Ecol 4, 175–188 (1977). https://doi.org/10.1007/BF02015075

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