Summary
The DNA nuclear content of 83 ‘pure’ intraductal cancers (DCIS), 30 DCIS with an invasive component, 7 intraductal papillomas, 26 atypical hyperplasias, and 5 ‘hobnail’ lesions was determined with a Cell Analysis Systems Image Analyzer (CAS 200). No consistent quantitative alteration of DNA or nuclear grade assessed by routine microscopy was observed for any ductal change diagnostic of DCIS. Fifty-eight percent of all DCIS were considered to be aneuploid, whereas all papillomas and atypical hyperplasias were diploid with normoproliferative S-phase and good nuclear grade. Use of ploidy, S phase, or nuclear grade for the distinction of DCIS and papillomas and atypical hyperplasia is limited, since their estimates would be comparable in 42%, 46%, and 40% of instances respectively. Poor nuclear grade was significantly more frequent in solid than cribriform types of DCIS. There were no significant differences in any parameters in DCIS with and without comedo necrosis. This represents further evidence against recognition of a unique comedo form of DCIS. DNA histograms of nuclei comprising the ‘hobnail’ lesions were aneuploid. Despite this their biologic nature is uncertain. No consistent differences in DNA content were noted between DCIS with and without an invasive component. This obfuscates insight into the role of DCIS in the development of invasive cancer of the breast.
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References
McDivitt RW, Stewart FW, Berg JW: Tumors of the breast. Atlas of tumor pathology, second series. Armed Forces Institute of Pathology, Washington DC, 1968, pp 14–49
Azzopardi JG: Problems in breast pathology. WB Saunders Co., Philadelphia, 1979, pp 113–166
Fisher ER: Relationship of fibrocystic disease to cancer of the breast. In: Hoogstratten B, McDivitt RW (eds) Breast Cancer, Vol I. Uniscience Series Experiences in Clinical Oncology. CRC Press, Boca Raton, Chapter VI, 1981, pp 119–136
Fisher ER, Sass R, Fisher B, Wickerham L, Paik SM: Pathologic findings from the National Surgical Adjuvant Breast Project (protocol 6). II. Intraductal carcinoma (DCIS). Cancer 57: 197–208, 1986
Page DL, Anderson TJ: Diagnostic histopathology of the breast. Churchill Livingstone, New York, 1987, pp 120–174
Carter D: Interpretation of breast biopsies. 2nd edition. Raven Press, New York, 1990, pp 63–115
Fechner RE, Mills SE: Breast pathology. Benign proliferations, atypias & in-situ carcinomas. ASCP Press, Chicago, 1990, pp 83–145
Fisher ER, Leeming R, Anderson S, Redmond C, Fisher B: Conservative management of intraductal carcinoma (DCIS) of breast. J Surg Oncol 47: 139–147, 1991
Tavassoli FA, Norris HJ: A comparison of the results of long-term follow-up for atypical intraductal hyperplasia and intraductal hyperplasia of the breast. Cancer 65: 518–529, 1990
Rosen PP, Braun DW JR, Kinne DW: The clinical significance of preinvasive breast carcinoma. Cancer 46: 919–925, 1980
Page DL, Dupont WD, Rogers LW, Landenberger M: Intraductal carcinoma of the breast; follow-up after biopsy only. Cancer 49: 751–758, 1982
Carpenter R, Gibbs N, Matthews J, Cooke T: Importance of cellular DNA content in pre-malignant breast disease and pre-invasive carcinoma of the female breast. Br J Surg 74: 905–906, 1987
Bacus S: An optical microscope image analysis system with clinical applications. Am Clin Products Rev, Feb 1988
Fisher ER: Identification of risk factors by conventional pathologic and some ancillary techniques in women with breast cancer. In: Ragaz J, Ariel IM (eds) High-Risk Breast Cancer. Springer-Verlag, New York, 1989, pp 233–250
Norris HJ, Bahr GF, Mikel UV: A comparative morphometric and cytophotometric study of intraductal hyperplasia and intraductal carcinoma of the breast. Anal Quant Cytol Histol 10: 1–9, 1988
Meyer JS: Cell kinetics of histologic variants of in-situ breast carcinoma. Breast Cancer Res Treat 7: 171–180, 1986
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Fisher, E.R., Siderits, R. Value of cytometric analysis for distinction of intraductal carcinoma of the breast. Breast Cancer Res Tr 21, 165–172 (1992). https://doi.org/10.1007/BF01974999
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DOI: https://doi.org/10.1007/BF01974999