Abstract
DNA from 30-million-year-old amber preserved termites (Mastotermes electrodominicus) was PCR amplified with nuclear ribosomal RNA small subunit primers and cloned into the TA vector (INVITROGEN). We obtained several classes of recombinant clones as a result. AuthenticMastotermes electrodominicus clones were identified. The source of other classes of clones was identified as contaminants of the ancient DNA template. Several of the clones appeared to be chimeric in structure with half of the clone identical to the termite sequence and the other half identical to contaminant sequences. The phenomenon of PCR jumping was identified as a possible source for the chimeric clones.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature
Paabo, S., Nature314 (1985) 644.
Doran, H. G., Dickel, D. N., Ballinger, W. E., Agee, P. J., and Laipis, W. W., Nature323 (1986) 803.
Lawlor, D., Dickel, C., Hauswirth, W., and Parham, P., Nature349 (1991) 785.
Higuchi, R., Dowman, B., Freiberger, M., Ryder, O., and Wilson, A. C., Nature312 (1984) 282.
Thomas, R. H., Schaffner, W., Wilson, A. C., and Paabo, S., Nature340 (1989) 465.
Thomas, W. K., Paabo, S., Villablanca, F. X., and Wilson, A. C., J. molec. Evol.31 (1990) 101.
Patton, J. L., and Smith, M. F., Evolution46 (1992) 148.
Golenberg, E. M., Giannasi, D. E., Clegg, M. T., Smiley, C. J., Durbin, M., Henderson, D., and Zurawski, G., Nature344 (1990) 656.
Hagelberg, E., Sykes, B., and Hedges, R., Nature342 (1989) 485.
Yokoi, T., Aoki, Y., and Sagisaki, K., Zs. Rechtsmedizin102 (1989) 323.
Williams, S. R., Longmire, J. L., and Beck, L. A., Am. J. Anthropol.812 (1990) 318.
Cooper, A., Mourer-Chauvire, C., Chambers, G. K., von Haeseler, A., Wilson, A. C., and Paabo, S., Proc. natl Acad. Sci. USA89 (1992) 8741.
Janeczewski, D. N., Yuhki, N., Gilbert, D. A., Jefferson, G. T., and O'Brien, S. J., Proc. natl Acad. Sci. USA89 (1992) 9769.
Cano, R. J., Poinar, H., and Poinar, G. O., Med. Sci. Res.20 (1992) 249.
Cano, R. J., Poinar, H., and Poinar, G. O., Med. Sci. Res.20 (1992) 619.
Cano, R. J., Poinar, H., Pieniazek, N., Aftrim, A., and Poinar, G. Jr, Nature363 (1993) 536.
DeSalle, R., Gatesy, J., Wheeler, W., and Grimaldi, D., Science257 (1992) 1933.
Maniatis, T., Fritsch, E. F., and Sambrook, J., Molecular Cloning: A Laboratory Manual. Cold Spring Harbour Press, Cold Spring Harbour, New York 1982.
Swofford, D. L., Phylogenetic Analysis Using Parsimony (PAUP). Illinois Natural History Survey, 1990.
Higuchi, R., and Wilson, A. C., Fedn Proc.43 (1984) 1557.
Paabo, S., Irwin, D. M., and Wilson, A. C., J. biol. Chem.265 (1990) 4718.
Bradley, R. D., Bull, J. J., and Hillis, D. M., personal communication, 1992.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
DeSalle, R., Barcia, M. & Wray, C. PCR jumping in clones of 30-million-year-old DNA fragments from amber preserved termites (Mastotermes electrodominicus). Experientia 49, 906–909 (1993). https://doi.org/10.1007/BF01952607
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01952607