Abstract
An oligomycin-resistant variant of human fibrosarcoma HT1080 was isolated and characterized as nuclear and codominant. The mutant was stable, was not cross-resistant to respiratory inhibitors, and it contained a mitochondrial ATPase which was less sensitive to oligomycin. Hybrids formed between the human mutant and a mouse cell line expressed the resistance phenotype. By a detailed karyotypic analysis of these hybrids using trypsin-Giemsa banding it was found that resistance to oligomycin correlated with the retention of two human chromosomes 10. The hybrid lines contained only mouse mitochondrial DNA as shown by analyses of mitochondrially synthesized proteins and mitochondrial DNA. The study assigns an ATPase oligomycin-resistance locus to human chromosome 10 and suggests that mouse and human subunits can combine in a functional enzyme complex.
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Literature cited
Downie, J.A., Gibson, F., and Cox, G.B. (1979).Annu. Rev. Biochem. 48:103:131.
Futai, M., and Konazawa, H. (1980).Curr. Top. Bioenerg. 10:181–215.
Tzagoloff, A., Macino, G., and Selbald, W. (1979).Annu. Rev. Biochem. 48:419–441.
Downie, J.A., Langmans, L., Cox, G.B., Yanofsky, C., and Gibson, P. (1980).J. Bacteriol. 143:8–17.
Downie, J.A., Cox, B.G., Lengmann, C., Ash, G., Becker, M., and Gibson, F. (1981).J. Bacteriol. 145:200–210.
Gay, N.J., and Walker, J.E. (1981).Nucleic Acids Res. 9:2187–2194.
Sebald, W. (1977).Biochim. Biophys. Acta 463:1–27.
Borst, P., and Grivell, L.A. (1978).Cell 15:705–723.
Lancashire, W.E., and Maltoon. (1979).Mol. Gen. Genet. 176:255–264.
Linnane, A.W., and Nagley, P. (1978).Plasmid 1:324–345.
Saunders, G.W., Bank, G.H., Kanstermann-Kuhn, B., and Hollenberg, C.P. (1979).Mol. Gen. Genet. 175:45–52.
Breen, G.A.M., and Scheffler, I.E. (1980).J. Cell Biol. 86:723–729.
Lagarde, A.E., and Siminovitch, L. (1979).Somat. Cell Genet. 5:847–871.
Kuhns, M.C., and Eisenstadt, J.M. (1979).Somat. Cell Genet. 5:821–832.
Howel, N., and Sager, R. (1979).Somat. Cell Genet. 5:833–845.
Lichtor, T., and Getz, G.S. (1978).Proc. Natl. Acad. Sci. U.S.A. 75:324–328.
Lichtor, T., Tung, B., and Getz, G.S. (1979).Biochemistry 18:2582–2590.
Ditta, G., Soderberg, K., Landy, F., and Scheffler I.E. (1976).Somat. Cell Genet. 2:331–344.
Breen, G.A.M., and Scheffler, I.E. (1979).Somat. Cell Genet. 5:441–451.
Soderberg, K., Mascerello, J.T., Breen, G.A.M., and Scheffler, I.E. (1979).Somat. Cell Genet. 5:225–240.
Macino, G.E., and Tzagoloff, A. (1980).Cell 20:507–517.
Macino, G.E., and Tzagoloff, A. (1979).Proc. Natl. Acad. Sci. U.S.A. 76:131–135.
Sebald, W., Wachter, E., and Tzagloff, A. (1979).Eur. J. Biochem. 100:599–607.
Glaser, E., Norling, B., and Ernster, L. (1981).Eur. J. Biochem. 115:189–196.
Aroskar, V.A., and Avadhani, N.G. (1979).Biochem. Biophys. Res. Commun. 19:17–23.
Dianoux, A.C., Bof, M., and Vignais (1978).Eur. J. Biochem. 88:69–77.
deJong, L., Holtrop, M., and Kroon, A.M. (1980).Biochim. Biophys. Acta. 606:331–337.
Koch, G. (1976).J. Biol. Chem. 251:6097–6107.
Sebald, W., Sebald-Althanus, M., and Wachter, E. (1977). InGenetics and Biogenesis of Mitochondria, Vol. 2, (eds.) Bandlow, W., Scheweyen, R.J., Wolf, K., and Kandewitz, F. (W. de Gruyter and Co., Berlin), pp. 433–440.
Anderson, S., Bankier, A.J., Barrell, B.G., de Bruijin, M.H.C., Coulson, A.R., Drouin, J., Eperson, I.C., Nierlich, D.P., Roe, B.A., Sanger, F., Schreier, P.H., Smith, A.J.H., Staden, R., and Young, I.G. (1981).Nature 290:457–465.
Creagan, R.P., and Ruddle, F.H. (1977). InMolecular Structure of Human Chromosomes, (ed.) Yunis, J.J. (Academic Press, New York), pp. 89–142.
McKusick, V.A., and Ruddle, F.H. (1977).Science 196:390–405.
Rasheed, S., Nelson-Rees, W.A., Toth, E.M., Arnstein, P., and Gardner, M.B. (1974).Cancer 33:1027–1033.
Kit, S., Dubbs, D.R., and Frearson, P.M. (1966).Int. J. Cancer 1:19–30.
Klebe, R.J., Chen, J.-R., and Ruddle, F.H. (1970).J. Cell Biol. 45:74–82.
Chen, T.R. (1977).Exp. Cell Res. 104:255–262.
Wigler, M.H., and Weinstein, I.B. (1975).Biochem. Biophys. Res. Commun. 63:669–674.
Shay, J.W. (1977).Proc. Natl. Acad. Sci. U.S.A. 74:2461–2464.
Scheffler, I.E. (1975).J. Cell Physiol. 85:293–306.
Francke, U., and Oliver, N. (1978).Hum. Genet. 45:137–165.
Ronne, M., Andersen, O., and Erlander, M. (1979).Hereditas 90:195–201.
Friend, K.K., Chen, S., and Ruddle, F.H. (1976).Somat. Cell Genet. 2:183–188.
Yatscoff, R.W., and Freeman, K.B. (1977).Can. J. Biochem. 55:1064–1074.
Bodenhagen, D., and Clayton, D.A. (1974).J. Biol. Chem. 249:7991–7995.
Laemmli, U.K. (1970).Nature 227:680–685.
Giles, R.E., Stoynowski, I., and Wallace, D.C. (1980).Somat. Cell Genet. 6:543–554.
Hirt, B. (1967).J. Mol. Biol. 26:365–369.
Shipman, C., Jr., Smith, S.H., and Droch, J.C. (1972).Proc. Natl. Acad. Sci. U.S.A. 69:1735–1757.
Southern, E.M. (1975).J. Mol. Biol. 98:503–517.
Rigby, P.W.J., Dieckmann, M., Rhodes, C., and Berg, P. (1977).J. Mol. Biol. 113:237–251.
Chang, A.C.Y., Lansman, R.A., Clayton, D.A., and Cohen, S.N. (1975).Cell 6:231–244.
Wallace, D.C., Bunn, C.L., and Eisenstadt, J.M. (1975).J. Cell Biol. 67:174–188.
Davidson, R.L., and Gerald, P.S. (1976).Somat. Cell Genet. 2:165–176.
O'Malley, K.A., and Davidson, R.L. (1977).Somat. Cell Genet. 3:441–448.
Kucherlapati, R.S., Hilwig, I., Geopp, A., and Ruddle, F.H. (1975).Humangenetik 27:9–14.
Croce, C.M. (1976).Proc. Natl. Acad. Sci. U.S.A. 3:441–448.
Weiss, M.C., and Green, H. (1967).Proc. Natl. Acad. Sci. 58:1104–1111.
Jami, J., and Grandchamp, S. (1971).Proc. Natl. Acad. Sci. U.S.A. 68:3097–3101.
DePierre, J.W., and Gurnster, L. (1977).Annu. Rev. Biochem. 46:201–262.
Brufani, M., Cellai, L., Musu, C., and Keller-Schierlein, W. (1972).Helv. Chim. Acta 55:2329–2346.
Lardy, H., Reed, P., Lin, C.H.C. (1975).Fed. Proc. 34:1707–1710.
Francke, U., and Gehring, U. (1980).Cell 22:657–664.
Wallace, D.C (1981).Mol. Cell Biol. 1:697–710.
Moore, K.H., Johnson, P.H., Chandler, L.E.W., and Grossman, L.I. (1977).Nucleic Acids Res. 4:1273–1281.
Francesco, L.D., Attardi, G., and Croce, C.M. (1980).Proc. Natl. Acad. Sci. U.S.A. 77:4079–4083.
Macino, G.E., and Tzagoloff, A. (1979).J. Biol. Chem. 254:4617–4623.
Wiseman, A., and Attardi, G. (1979).Somat. Cell Genet. 5:241–262.
Mascarello, J.T., Soderberg, K., and Scheffler, I.E., (1980).Cytogenet. Cell Genet. 28:121–135.
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Webster, K.A., Oliver, N.A. & Wallace, D.C. Assignment of an oligomycin-resistance locus to human chromosome 10. Somat Cell Mol Genet 8, 223–244 (1982). https://doi.org/10.1007/BF01538679
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DOI: https://doi.org/10.1007/BF01538679