Summary
Four inbred lines ofZea mays (33.16, B 68, N 7B, B 77) were grown in nutrient solution to which K2Cr2O7 was added to give final concentration of 5 mg/l Cr (VI). The most evident differences in metal tolerance were observed between the B 68 and 33.16 line: in fact, even though the level of Cr (VI) was almost the same in the root tissues of both lines after 6 d of treatment, in the B 68 line, Cr induced marked alterations of nuclear structure and a progressive arrest of the cell cycle in G 1. In the 33.16 line, on the contrary, the integrity of the nuclei was well preserved and the progression of the cell cycle was only barely affected.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- Cr:
-
(VI) hexavalent chromium
- CRBC:
-
chick red blood cells
- DAPI:
-
4′,6-diamidino-2-phenylindole
- FCM:
-
Flow cytometry
- FM:
-
Fluorescence microscopy
- TEM:
-
Transmission electron microscopy
- Tris:
-
10 mM Tris(hydroxymethyl)aminomethane
References
Albergoni V, Piccinini E, Cappellotti O (1980) Response to heavy metals in organisms — I: excretion and accumulation of physiological and nonphysiological metals inEuglena gracilis. Comp Biochem Physiol 62: 121–127
Arnon DI, Hoagland DR (1940) Crop production in artificial culture solution and soils with special reference to factors influencing yield and absorption of organic nutrients. Soil Sci 50: 463–483
Baisch H, Gohde W, Linden VA (1975) Analysis of PCP-data to determine the fraction of cells in the various phases of the cell cycle. Radiat Environ Biophys 12: 31–39
Baker AJM (1987) Metal tolerance: New Phytol 106 [Suppl]: 93–111
Barcelo J, Poschenrieder Ch, Gunse B (1985) Effect of chromium on mineral element composition of bush bean. J Plant Nutr 8: 211–217
Bassi M, Corradi MG, Favali MA (1990) Effects of chromium in freshwater algae and macrophytes. In: Wang W, Gorsuch JW, Lower WR (eds) Plants for toxicity assessment. ASTMSTP 1091. American Society for Testing and Materials, Philadelphia, pp 204–224
— —, Realini M (1990) Effects of chromium (VI) on two freshwater plants,Lemna minor andPistia stratiotes. I-Morphological observations. Cytobios 62: 27–38
Bronzetti G, Galli A, Boccardo P, Vellosi R, Del Carratore R (1986) Genotoxicity of chromium in vitro on yeast: interaction with DNA. Toxicol Environ Chem 13: 103–111
Corradi MG, Musetti R, Lorenzoni C, Favali MA (1989) Risposta di linee geneticamente diverse diZea mays al trattamento con Cr (VI). In: Abstracts VIII ABCD Congress, p 204
Fasulo MP, Bassi M, Donini A (1982) Cytotoxic effects of hexavalent chromium inEuglena gracilis, I. First observations. Protoplasma 110: 39–47
— — — (1983) Cytotoxic effects of hexavalent chromium inEuglena gracilits (1983) II. Physiological and ultrastructural studies. Protoplasma 114: 35–43
Favali MA, Barbieri N, Bianchi A, Bonecchi R, Conti M (1980) X-ray microanalysis on leaf tumours from maize plants experimentally infected with maize rough dwarf virus (MRDV), Virology 103: 375–368
—, Osler R, Loi N, Refatti E (1985) Ultrastructure and X-ray microanalysis on maize leaves infected with barley yellow dwarf virus. Mitt Biol Bundesanstalt 228: 21–26
- Mazzo L, Ferrari G, Gerola FM (1986) Ultrastructural and physiological responses of the green algaeCoccomyxa andScenedesmus to copper. In: Proceedings Eleventh Int Congr Electron Microscopy, pp 3259–3260
Halls DJ, Stoeppler CM, Stoeppler M (1987) Application of rapid furnace programmes in atomic absorption spectrometry to the determination of lead, chromium and copper in digests of plant materials. Analyst 112: 185–189
Levi M, Tarquini F, Sgorbati S, Sparvoli E (1986) Determination of DNA content by static cytofluorimetry in nuclei released from fixed plant tissue. Protoplasma 132: 64–68
Levis AG, Bianchi V, Buttignol F, Majone F, Tamino G, Pegoraro B, Motion C, Sponza G, Saggioro D, Matassi G (1976) Cytogenetic effects and mechanisms of action of chromium compounds. Atti Assoc Genet Ital 21: 80–84
— —, Tamino G, Pegoraro B (1978) Cytotoxic effects of hexavalent and trivalent chromium on mammalian cells in vitro. Br J Cancer 37: 386–395
Mangi J, Schmidi K, Pankow J, Gaines L, Turner P (1978) Effects of chromium on some aquatic plants. Environ Pollut 16: 285–291
Majone F (1977) Effects of potassium dichromate on mitosis of cultured mammalian cells. Caryologia 30: 469–481
— (1981) Cytotoxic and clastogenic effects of soluble and insoluble compounds containing hexavalent and trivalent chromium. Br J Cancer 44: 219–235
Osler R, Loi N, Lorenzoni C, Snidaro M, Refatti E (1985) Barley Yellow Dwarf virus infections in maize (Zea mays L.) inbreds and hybrids in northern Italy. Maydica 30: 285–299
Sgorbati S, Levi M, Sparvoli E, Trezzi F, Lucchini G (1986) Cytometry and flow cytometry of 4′,6-diamidino-2-phenylindole (DAPI)-stained suspensions of nuclei released from fresh and fixed tissues of plants. Physiol Plant 68: 471–476
Slater ML, Sharrow SO, Gart JJ (1977) Cell cycle ofSaccharomyces cerevisiae in populations growing at different rates. Proc Natl Acad Sci USA 74: 3850–3854
Vazquez MD, Poschenrieder Ch, Barcelo J (1987) Chromium VI induced structural and ultrastructural changes in bush bean plants (Phaseolus vulgaris L.). Ann Bot 59: 427–438
Zaccheo P, Cocucci M, Cocucci S (1985) Effects of Cr on proton extrusion, potassium uptake and transmembrane electric potential in maize root segments. Plant Cell Environ 8: 721–726
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Corradi, M.G., Levi, M., Musetti, R. et al. The effect of Cr (VI) on different inbred lines ofZea mays . Protoplasma 162, 12–19 (1991). https://doi.org/10.1007/BF01403896
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01403896