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Expression and analysis of the NS2 protein of influenza A virus

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Summary

Influenza NS2 protein was expressed inSaccharomyces cerevisiae using a copper-inducible promoter. The protein produced had a molecular weight of 13 kDa, was reactive with anti-NS2 antiserum and was localised to the yeast cell nucleus. Two-hybrid analysis identified a direct protein-protein interaction between NS2 and the M2 protein of the virus, involving the C-terminal 163 residues of M1. A filter-binding assay localised the M1 binding region to the C-terminal 70 amino acids of NS2.

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Authors and Affiliations

  1. Biomolecular Research Institute, Parkville

    A. C. Ward, L. A. Castelli, A. C. Lucantoni, A. A. Azad & I. G. Macreadie

  2. Russell Grimwade School of Biochemistry, University of Melbourne, Parkville

    A. C. Ward

  3. CSIRO Division of Biomolecular Engineering, Parkville, Victoria, Australia

    J. F. White

Authors
  1. A. C. Ward
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  2. L. A. Castelli
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  3. A. C. Lucantoni
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  4. J. F. White
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  5. A. A. Azad
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  6. I. G. Macreadie
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Ward, A.C., Castelli, L.A., Lucantoni, A.C. et al. Expression and analysis of the NS2 protein of influenza A virus. Archives of Virology 140, 2067–2073 (1995). https://doi.org/10.1007/BF01322693

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  • DOI: https://doi.org/10.1007/BF01322693

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