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A glutamate-sensitive neuronal system originating from the area postrema terminates in and transports acetylcholinesterase to the nucleus of the solitary tract

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Journal of Neurocytology

Summary

The possible cellular mechanism of action of systemically administered monosodium-l-glutamate and the projections of glutamate-sensitive area postrema neurons have been studied in rats. Parenteral administration of monosodium-l-glutamate induced a selective degeneration of a particular population of AChE-containing area postrema neurons. Electron microscopic cytochemistry and X-ray microanalysis revealed the presence of calcium-containing electron-dense deposits in the mitochondria of degenerating area postrema neurons indicating the possible pathogenetic role of an enhanced intracellular calcium level in the mechanism of monosodium-l-glutamate-induced nerve cell degeneration. Degeneration of area postrema neurons was followed by the appearance of degenerating axon terminals in a well-defined region of the nucleus of the solitary tract, the area subpostrema. Degenerating area postrema neurons and axon terminals were rapidly engulfed by phagocytes predominantly of microglial character. AChE activity, localized to the basal lamina of the capillaries of the area subpostrema under normal conditions, could no longer be detected in rats treated with monosodium-l-glutamate 3–4 weeks previously.

These findings provide evidence for the existence of a particular population of glutamate-sensitive, AChE-containing area postrema neurons which project and transport AChE to the nucleus of the solitary tract. This specific neuronal pathway connecting the area postrema with the nucleus of the solitary tract may play an important role in some of the functions attributed to the area postrema. The results also strengthen the hypothesis that brain capillary AChE activity may be of neuronal origin.

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Authors and Affiliations

  1. Endocrine Unit, First Department of Medicine, University Medical School, Szeged, Hungary

    Sarolta Karcsú & Ferenc A. László

  2. Department of Physiology, University Medical School, Szeged, Hungary

    Gábor Jancsó

  3. Department of Anatomy, University Medical School, Szeged, Hungary

    Lajos Tóth & Elizabeth Király

  4. Max Planck Institute for Psychiatry, Munich, FRG

    Georg W. Kreutzberg

  5. Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary

    Ernö Bácsy

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  1. Sarolta Karcsú
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  2. Gábor Jancsó
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  4. Lajos Tóth
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  7. Ferenc A. László
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Karcsú, S., Jancsó, G., Kreutzberg, G.W. et al. A glutamate-sensitive neuronal system originating from the area postrema terminates in and transports acetylcholinesterase to the nucleus of the solitary tract. J Neurocytol 14, 563–578 (1985). https://doi.org/10.1007/BF01200798

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  • DOI: https://doi.org/10.1007/BF01200798

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