Summary
A preliminary attempt was made for producing vitamin B-12 byMethanosarcina barkeri strain Fusaro in a fed-batch culture with a methanol minimum medium. After 11 days, total methanol consumption, cell density and corrinoid concentration were 145 g/l, 8.5 g(dry cell weight)/l, and 135 mg/l (73% in supernatant) respectively. Electrophoretic separation revealed that 33% of the total corrinoids was B-12 Factor III (5-hydroxybenzimidazolylcobamide) and the remaining corrinoids were cobinamide (Factor B) and its derivatives.
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Mazumder, T.K., Nishio, N., Hayashi, M. et al. Production of corrinoids including vitamin B-12 byMethanosarcina barkeri growing on methanol. Biotechnol Lett 8, 843–848 (1986). https://doi.org/10.1007/BF01078642
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DOI: https://doi.org/10.1007/BF01078642