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Lipase catalysed isomerisation of 1,2-(2,3)-diglyceride into 1,3-diglyceride. The crucial role of water

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Summary

Enzyme catalyzed isomerisation of 1,2-dipalmitin into the 1,3-isomer is shown to occur with 1,3 regiospecific lipases. The mechanism of this transformation is elucidated and the crucial role of the water still present in the enzyme preparation is shown. Thus, the first step is the hydrolysis of the dipalmitin followed by the isomerisation of the 2-monopalmitin into the 1-monoester. The last is then esterified into the 1,3-dipalmitin.

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Authors and Affiliations

  1. Laboratoire de RMN et Réactivité Chimique-URA CNRS 472, Faculté des Sciences et des Techniques, 2, rue de la Houssinière, 44072, Nantes cédex 03, France

    Andreas Heisler, Claude Rabiller & Laurence Hublin

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  1. Andreas Heisler
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  2. Claude Rabiller
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  3. Laurence Hublin
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Heisler, A., Rabiller, C. & Hublin, L. Lipase catalysed isomerisation of 1,2-(2,3)-diglyceride into 1,3-diglyceride. The crucial role of water. Biotechnol Lett 13, 327–332 (1991). https://doi.org/10.1007/BF01027677

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