Summary
To elucidate the histogenesis of sclerosis haemangioma of the lung, we examined 7 cases with the immunoperoxidase method using antibodies against several useful marker antigens; secretory component (SC), cytokeratins, epithelial membrane antigen (EMA) for epithelial cells, factor VIII related antigens (factor VIII) for endothelial cells, vimentin and desmin for mesenchymal cells. The results were compared with those of histologically normal lung tissues. Both the characteristic round cells arranged in sheets, which are present predominantly in the solid area and are reported to be neoplastic, and the flattened cells lining blood lakes show positive staining for EMA only, with negative staining for the other marker antigens. These observations suggest that these cells are derived from epithelium rather than mesothelium or from endothelium, and are analogous to type I pneumocytes. This conclusion is supported by their immunohistochemical characteristics, in comparison with the localization patterns of the marker antigens in normal lung tissues. However, the lining epithelial cells of papillary projections in the papillary area and of ducts in the solid area stained for SC and cytokeratins as well as EMA, and their immunohistochemical characteristics are analogous to those of bronchiolar epithelial cells or type II pneumocytes in normal lung tissues.
Similar content being viewed by others
References
Adamson IYR, Bowden DH (1974) The type 2 cells as progenitor of alveolar epithelial regeneration. A cytodynamic study in mice after exposure to oxygen. Lab Invest 30:35–42
Altmannsberger M, Osborn M, Schauer A, Weber K (1981) Antibodies to different intermediate filament proteins. Cell type-specific markers on paraffin-embedded human tissues. Lab Invest 45:427–434
Alverez-Fernandez E, Escalona-Zapata J (1981) Sclerosing haemangioma of the lung. A histochemical, electron microscopical, tissue culture and time-lapse cinematographic study. Histopathology 5:579–588
Blobel GA, Moll R, Franke WW, Vogt-Moykopf I (1984) Cytokeratins in normal lung and lung carcinomas. I. Adenocarcinomas, squamous cell carcinomas and culture cell lines. Virchows Archiv [Cell Pathol] 45:407–429
Corson JM, Pinkus GS (1982) Mesothelioma: Profile of keratin proteins and carcinoembryonic antigen. An immunoperoxidase study of 20 cases and comparison with pulmonary adenocarcinomas. Am J Pathol 108:80–87
Delsol G, Gatter KC, Stein H, Erber WN, Pulford KAF, Zinne K, Mason DY (1984) Human lymphoid cells express epithelial membrane antigen. Lancet ii:1124–1126
Gabbiani G, Kapanci Y, Barazzone P, Franke WW (1981) Immunochemical identification of intermediate-sized filaments in human neoplastic cells. A diagnostic aid for the surgical pathologist. Am J Pathol 104:206
Haas JE, Yunis EJ, Totten RS (1972) Ultrastructure of a sclerosing hemangioma of the lung. Cancer 30:512–518
Haimoto H, Nagura H, Imaizumi M, Watanabe K, Iijima S (1984) Immunoelectronmicroscopic study on the transport of secretory IgA in lower respiratory tract and alveoli. Virchows Arch [Pathol Anat] 404:369–380
Hill GS, Eggleston JC (1972) Electron microscopic study of so-called “pulmonary sclerosing hemangioma”: Report of a cases suggesting epithelial origin. Cancer 30:1092–1106
Katzenstein AA, Gmelich JT, Carrington CB (1980) Sclerosing hemangioma of the lung: A clinicopathologic study of 51 cases. Am J Surg Pathol 4:343–356
Katzenstein AA, Fulling K, Weise DL, Battifora H (1983) So-called sclerosing hemangioma of the lung. Evidence for mesothelial origin. Am J Surg Pathol 7:3–14
Kay S, Still WJS, Borochovitz D (1977) Sclerosing hemangioma of the lung: an endothelial or epithelial neoplasm? Hum Pathol 8:468–474
Kennedy A (1973) ‘Sclerosing haemangioma’ of the lung: an alternative view of its development. J Clin Pathol 26:792–799
Koide T (1979) Ultrastructural analyses of 7 cases of sclerosing hemangioma of the lung (Liebow) with a special reference to histogenesis. Haigan (Lung Cancer) 19:19–36 (in Japanese)
Liebow AA, Hubbell DS (1956) Sclerosing hemangioma (histiocytoma, xanthoma) of the lung. Cancer 9:53–75
McLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative. A new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077–1083
Mukai K, Rosai J, Burgdorf WHC (1980) Localization of factor VIII-related antigen in vascular endothelial cells using an immunoperoxidase method. Am J Surg Pathol 4:273–276
Nagura H, Tsutsumi Y, Shioda Y, Watanabe K (1983) Immunohistochemistry of gastric carcinoma and associated diseases. Novel distribution of carcinoembryonic antigen and secretory component of the surface of gastric cancer cells. J Histochem Cytochem 31:193–198
Nakane PK (1975) Recent progress in peroxidase-labeled antibody method. Ann NY Acad Sci 254:203–211
Palacios JJN, Escribano PM, Toledo J, Garzon A, Larru E, Palomera J (1979) Sclerosing hemangioma of the lung. An ultrastructural study. Cancer 44:949–955
Poger ME, Hirsch BR, Lamm ME (1976) Synthesis of secretory component by colonic neoplasms. Am J Pathol 82:327–338
Rosai J (1981) Ackerman's Surgical Pathology. C.V. Mosby Company, St. Louis, p 257
Said JW, Nash G, Banks-Schlegel S, Sassoon AF, Murakami S, Shintaku IP (1983) Keratin in human lung tumors. Patterns of localization of different molecular weight keratin proteins. Am J Pathol 113:27–32
Schlegel RS, Banks-Schlegel S, McLeod JA, Pinkus GS (1980) Immunoperoxidase localization of keratin in human neoplasms. A preliminary survey. Am J Pathol 101:41–50
Sehested M, Hou-Jensen K (1981) Factor VIII related antigen as an endothelial cell marker in benign and malignant diseases. Virchows Arch [Pathol Anat] 391:217–225
Shioda Y, Nagura H, Tsutsumi Y, Shimamura K, Tamaoki N (1984) Distribution of Leu 7(HNK-1) antigen in human digestive organs: an immunohistochemical study with monoclonal antibody. Histochem J 16:843–854
Singh G, Katyal SL, Ordonez NG, Dail DH, Negishi Y, Weeden VW, Marcus PB, Weldon-Linne M, Axiotis CA, Alvarez-Fernandez E, Smith WI (1984) Type II pneumocytes in pulmonary tumors. Arch Pathol Lab Med 108:44–48
Sloane JP, Ormerod MG (1981) Distribution of epithelial membrane antigen in normal and neoplastic tissues and its value in diagnostic tumor pathology. Cancer 47:1786–1795
Sloane JP, Hughes F, Ormerod MG (1983) An assessment of the value of epithelial membrane antigen and other markers in solving diagnostic problems in tumour histopathology. Histochem J 15:645–654
Spencer H (1977) Pathology of the lung. Philadelphia, W.B. Saunders Company, pp 933–936
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Haimoto, H., Tsutsumi, Y., Nagura, H. et al. Immunohistochemical study of so-called sclerosing haemangioma of the lung. Vichows Archiv A Pathol Anat 407, 419–430 (1985). https://doi.org/10.1007/BF00709988
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00709988