Zusammenfassung
Es wurde mit der von Ernst (1975) beschriebenen Methode die ultrastrukturelle Lokalisation der Na-K-ATPase in der Prominentia spiralis des Meerschweinchens untersucht. Die Enzymaktivität war auf das Oberflächenepithel der Prominentia spiralis beschränkt. Die Befunde werden diskutiert.
Summary
The ultrastructural localization of the phosphatase component of Na-K-ATPase in the guinea pig spiral prominence was studied utilizing p-nitrophenyl phosphate (NPP) as substrate. Na-K-ATPase activity was limited to the epithelial cells. The results are discussed.
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References
Borghesan E (1957) Modality of the cochlear humoral circulation. Laryngoscope 67: 1266–1285
Ernst SA (1972) Transport adenosine triphosphatase cytochemistry. I. Biochemical characterization of a cytochemical medium for the ultrastructural localization of ouabain-sensitive, potassium-dependent phosphatase activity in the avian salt gland. J Histochem Cytochem 20: 13–22
Ernst SA (1975) Transport ATPase cytochemistry: Ultrastructural localization of potassium-dependent and potassium-independent phosphatase activities in rat kidney cortex. J Cell Biol 66: 586–608
Hinojosa R (1971) Electron microscope studies of the stria vascularis and spiral ligament after ferritin injection. Acta Otolaryngol (Stockh) 74: 1–14
Iinuma T (1967) Evaluation of adenosine triphosphatase activity in the stria vascularis and spiral ligament of normal guinea pigs. Laryngoscope 77: 141–158
Ilberg C von, Spoendlin H, Vosteen KH (1968) Die Ultrastruktur und Funktion des Sulcus spiralis externus und der Prominentia spiralis der Meerschweinchenschnecke. Darstellung mittels Thorotrast. Arch Klin Exp Ohren-Nasen-Kehlkofheilkd 192: 124–136
Ishii T, Nomura Y (1968) Histochemical study on adenosine triphosphatase activity in the inner ear. Pract Otorhinolaryngol 30: 237–244
Jahnke K, Rudert H (1973) Elektronenmikroskopische Untersuchungen über die Aufnahme von Proteinen aus der Endolymphe. Arch Klin Exp Ohren-Nasen-Kehlkopfheilkd 205: 179–182
Kuijpers W, Bonting SL (1969) Studies on (Na+-K+)-activated ATPase. XXIV. Localization and properties of ATPase in the inner ear of the guinea pig. Biochim Biophys Acta 173: 477–485
Mees K (1981) On the function of the spiral prominence. An HRP study in the guinea pig. 18th Workshop on Inner Ear Biology. Montpellier-La Grande Motte, France, September 14–16, 1981
Mees K (1982) Ultrastructural localization of K+-dependent, ouabain-sensitive NPPase (Na-K-AT-Pase) in the guinea pig inner ear. Acta Otolaryngol (Stockh) (in press)
Nomura Y, Ishii T, Ishii D (1970) The histochemistry of the spiral prominence. Arch Klin Exp Ohren-Nasen-Kehlkopfheilkd 195: 266–275
Rudert H (1969) Experimentelle Untersuchungen zur Resorption der Endolymphe im Innenohr des Meerschweinchens. II. Versuche mit radioaktiv markierten Substanzen und ihre autoradiographische Auswertung. Arch Klin Exp Ohren Nasen Kehlkopfheilk 193: 156–170
Spoendlin H (1967) Spiral prominence. In: Jurato S (ed) Submicroscopic structure of the inner ear. Pergamon Press, Oxford, pp 149–160
Yamamoto K, Nakai Y (1964) Electronmicroscopic studies on the functions of the stria vascularis and the spiral ligament in the inner ear. Ann Otol 73: 332–347
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Mees, K. Ultrastructural localization of Na-K-ATPase in the spiral prominence epithelium of the guinea pig. Arch Otorhinolaryngol 237, 35–40 (1982). https://doi.org/10.1007/BF00453714
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DOI: https://doi.org/10.1007/BF00453714