Abstract
N-Methyl-N-formylhydrazine was administered in drinking water as a 0.0039% solution to randomly bred Swiss albino mice for life starting from 6 weeks of age. The compound induced tumors of lungs, livers, blood vessels, gall bladder and bile ducts. The tumor incidences in these five tissues were 77, 46, 21, 10 and 7%, while in the untreated controls they were 18, 1, 6, 0 and 0%, respectively. Histopathologically, the tumors were classified as adenomas and adenocarcinomas of lungs, benign hepatomas and liver cell carcinomas, angiomas and angiosarcomas of blood vessels, adenomas and adenocarcinomas of gall bladder and cholangiomas. The macroscopic and light microscopic involvement of the tissues with the tumors are described and some of them are illustrated. N-Methyl-N-formylhydrazine is an ingredient of the edible mushroom, the false morel Gyromitra esculenta. The findings are discussed from the viewpoint of a potential human health hazard.
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References
Armitage, P. 1971. Statistical Interference. In: Statistical Methods in Medical Research, Blackwell Scientific Publication, Oxford, England, pp. 135–138.
Dearness, J. Gyromitra Poisoning. Mycologia, 1924. 16: 199.
Delia Porta, G., P. Shubik & V. Scortecci. 1959. The Action N-2-Fluorenyl-acetamide in the Syrian Golden Hamster. J. Natl. Cancer Inst., 22: 463–487.
Edmonson, H.A. 1958. Tumors of the Liver and Intrahepatic Bile Ducts. In: Atlas of Tumor Pathology, Armed Forces Inst. Pathol. sect. VII, fasc. 25. Washington, D.C.
Evans, R.W. 1968. Histological Appearances of Tumors. Williams and Wilkins, Baltimore.
Faure, J., A. Beaudoing & G. Cau. 1965. A Propos d'une Intoxication Mortelle par les Fausses Morilles (Gyromitra esculenta). Bull. Med. Leg. Tox., 8: 83–85.
Franke, S., U. Freimuth & P.H. List. 1967. Über die Giftigkeit der Frühjahrslorchel Gyromitra (Helvella) esculenta. Fr. Arch. Toxicol., 22: 293–332.
Giusti, G.V. & A. Carnevale. 1974. A case of fatal poisoning by Gyromitra esculenta. Arch. Toxicol., 33: 49–54.
Kalmár, Z. & Gy. Makara. 1973. Ehetö és Mérges Gombák. Mezögazdasági Kiadó, 3rd edition. Budapest, Hungary.
Klosterman, H. 1974. Vitamin B6 antagonists of natural origin. J. Agr. Food Chem., 22: 13–16.
List, P.H. & P. Luft. 1968. Gyromitrin, das Gift per Frühjahrslorchel. Arch. Pharm., 301: 294–305.
List, P.H. & P. Luft. 1968. Gyromitrin, das Gift der Frühjahrslorchel Helvella (Gyromitra) esculenta Pers. ex. FR. Zeitsch. Pilzkunde, 34: 3–8.
List, P.H. & P. Luft. 1969. Nachweiss und Gehaltsbestimmung von Gyromitrin in frischen Lorcheln. Arch. Pharm., 302: 143–146.
Magee, P.N. & J.M. Barnes. 1956. The production of malignant primary hepatic tumors in the rat by feeding dimethylnitrosamine. Brit. J. Cancer, 10: 114–122.
Miller, O.K. 1972. Mushrooms of North America. Dutton and Co., Inc. New York.
Nagel, D., L. Wallcave, B. Toth & R. Kupper. 1977. Formation of methylhydrazine from acetaldehyde N-methyl-N-formylhydrazone a component of Gyromitra esculenta. Cancer Res., 37: 3458–3460.
Niskanen, A., H. Pyysalo, E. Rimaila-Pärnänen & P. Hartikka. 1976. Short-term peroral toxicity of ethylidene gyromitrin in rabbits and chickens. Foord Cosmet. Toxicol. 14: 409–415.
Pyysalo, H. 1975. Some new toxic compounds in false morels,Gyromitra esculenta. Naturwissensch., 62: 395.
Pyysalo, H. 1976. Tests for Gyromitrin, a poisonous compound in false morel Gyromitra esculenta. Z. Lebensm. Unters. Forsch., 160: 325–330.
Pyysalo, H. & E. Honkanen. 1976. Mass spectra of some N-methyl-N-formylhydrazones. Acta Chem. Scand., 30: 792–793.
Pyysalo, H. & A. Niskanen. 1977. On the occurrence of N-methyl-N-formylhydrazones in fresh and processed false morel, Gyromitra esculenta. J. Agric. Food Chem., 25: 644–647.
Savonius, M. 1973. Mushrooms and Fungi. Crown, New York.
Schmidlin-Mészáros, J. 1974. Gyromitrin in Trockenlorcheln (Gyromitra esculenta sice.). Mitt. Geb. Lebersm. Hyg. 65:453–465.
Simmons, D.M. 1971. The mushroom toxins. Del. Med. J., 43: 177–187.
Toth, B. 1972. A toxicity method with calcium cyclamate for chronic carcinogenesis experiments. Tumori, 58: 137–142.
Toth, B. 1972. Hydrazine, methylhydrazine and methylhydrazine sulfate carcinogenesis in Swiss Mice. Failure of ammonium hydroxide to interfere in the development of tumors. Int. J. Cancer, 9: 109–118.
Toth, B. 1975. Synthetic and naturally occurring hydrazines as possible cancer causative agents. Cancer Res., 35: 3693–3697.
Toth, B. 1977. Tumor induction studies with hydrazine derivative ingredients of mushrooms, Agaricus bisporus and Gyromitra esculenta. 4th meeting of Europ. Assoc. Cancer Res., Abstracts and programme, 68.
Toth, B. 1978. Additional series of carcinogenic hydrazines. XII Intern. Cancer Congress. Buenos Aires, Argentina, Abstracts, 1: 30–31.
Toth, B. & D. Nagel. 1977. Cancer induction with N-methyl-N-formylhydrazine, an ingredient of the false morel. Presented at the II. Intern. Mycol. Congress, 2: 674.
Toth, B. & D. Nagel. 1978. Tumors induced in mice by N-methyl-N-formylhydrazine of the false morel, Gyromitra esculenta. J. Natl. Cancer Inst., 60: 201–204.
Toth, B. & H. Shimizu. 1973. Methylhydrazone tumorigensis in Syrian golden hamsters and the morphology of malignant histiocytomas. Cancer Res., 33: 2744–2753.
Toth, B. & H. Shimizu. 1974. 1-Carbamyl-2-phenylhydrazine tumorigenesis in Swiss Mice. Morphology of Lung Adenomas. J. Natl. Cancer Inst., 52: 241–251.
Toth, B., L. Tomatis & P. Shubik. 1961. Multipotential carcinogenesis with Urethan in the Syrian golden hamster. Cancer Res., 21: 1537–1541.
Wright, A.V., A. Niskanen & H. Pyysalo. 1977. The toxicities and mutagenic properties of ethylidene gyromitrin and N-methylhydrazine with escherichia coli as test organism. Mut. Res., 56: 105–110.
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Toth, B., Patil, K., Erickson, J. et al. False morel mushroom gyromitra esculenta toxin: N-methyl-N-formylhydrazine carcinogenesis in mice. Mycopathologia 68, 121–128 (1979). https://doi.org/10.1007/BF00441091
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DOI: https://doi.org/10.1007/BF00441091