Summary
A gene bank of a yeast wild type DNA in the high copy number vector YEp 13 was screened for recombinant plasmids which suppress the mitochondrial RNA splice defect exerted by mutant M1301, a −1 by deletion in the first intron of the mitochondrial COB gene (bIl). A total of 17 recombinant plasmids with similar suppressor activity were found. Restriction mapping and cross-hybridization of the inserts revealed that these 17 plasmids contain three different inserts, all lacking any extended sequence homology. Each of the inserts, when present in high copy number, has a similar suppressor activity: high in the presence of mutation M1301 in bll, a group II intron, and low but significant with the presence of few mutants in bI2 and bI3 of the COB gene, both of which are group I introns.
Similar content being viewed by others
References
Atkins NS, Dunst RW, Hopper A (1985) Mol Cell Biol 5:907–915
Baldari C, Cesareni G (1985) Gene 35:27–32
Bechmann H, Haid A, Schweyen RJ, Mathews S, Kaudewitz F (1981) J Biol Chem 256:3525–3531
Beggs JD (1978) Nature 275:104–109
Broach JR, Strathern JN, Hicks J (1979) Gene 8:121–123
Cryer DR, Eccleshall R, Marmur J (1975) In: Prescott DM (ed) Methods in cell biology, vol XII. Academic Press, New York, pp 39–44
Dujardin G, Pajot P, Groudinsky O, Slonimski PP (1980) Mol Gen Genet 179:469–482
Dinter-Gottlieb G (1986) Proc Natl Acad Sci USA 83:6250–6254
Groudinsky O, Dujardin G, Slonimski PP (1981) Mol Gen Genet 184:493–503
Hohmann S, Zimmermann R (1986) Curr Genet 11:217–225
Holl J, Rödel G, Schweyen RJ (1985) EMBO J 4:2081–2085
Kovacava V, Irmerlova J, Kovac LV (1968) Biochim Biophys Acta 162:157–163
Lancashire WE, Mattoon JR (1979) Mol Gen Genet 70:333–344
Lazowska J, Jacq C, Slonimski PP (1980) Cell 22:333–348
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, New York
Michel F, Dujon B (1983) EMBO J 2:33–38
Montgomery DL, Boss JM, McAndrew SJ, Marr L, Walthall DA, Zitomer RS (1982) J Biol Chem 257:7756–7761
Rigby PW, Dieckmann M, Rhodes C, Berg P (1977) J Mol Biol 113:237–251
Rodriguez JR, Tait RC (1983) Recombinant DNA-Techniques. Addison-Wesley, London, Amsterdam, pp 171–172
Schmelzer C, Haid A, Grosch G, Schweyen RJ, Kaudewitz F (1981) J Biol Chem 256:7610–7619
Schmelzer C, Schmidt C, May K, Schweyen RJ (1983) EMBO J 2:2047–2052
Schmidt C, Söllner T, Schweyen RJ (1987) Mol Gen Genet 210: 145–152
Söllner T, Schmidt C, Schmelzer C (1987) Curr Genet 12: 497–501
Stinchcomb DT, Mann C, Davis RW (1982) J Mol Biol 158:157–179
Struhl K, Stinchcomb DT, Scherer S, Davis RW (1979) Proc Natl Acad Sci USA 76:1035–1039
Weiss-Brummer B, Rödel G, Schweyen RJ, Kaudewitz F (1982) Cell 29:527–536
Winston F, Chumley F, Fink DR (1983) Recombinant DNA, Part C. In: Wu R, Grossman L, Moldare K (eds) Methods Enzymol 101:211–228
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Koff, H., Schmidt, C., Wiesenberger, G. et al. Three nuclear genes suppress a yeast mitochondrial splice defect when present in high copy number. Curr Genet 12, 503–509 (1987). https://doi.org/10.1007/BF00419559
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00419559