Skip to main content
Log in

Effects of water stress on photosynthetic electron transport, photophosphorylation, and metabolite levels of Xanthium strumarium mesophyll cells

  • Published:
Planta Aims and scope Submit manuscript

Abstract

Several component processes of photosynthesis were measured in osmotically stressed mesophyll cells of Xanthium strumarium L. The ribulose-1,5-bisphosphate regeneration capacity was reduced by water stress. Photophoshorylation was sensitive to water stress but photosynthetic electron transport was unaffected by water potentials down to-40 bar (-4 MPa). The concentrations of several intermediates of the photosynthetic carbon-reduction cycle remained relatively constant and did not indicate that ATP supply was limiting photosynthesis in the water-stressed cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Abbreviations

Hepes:

4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid

PGA:

3-phosphoglyceric acid

RuBP:

ribulose-1,5-bisphosphate

References

  • Arnon, D.I. (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24, 1–15

    Google Scholar 

  • Beadle, C.I., Nielson, R.E., Jarvis, P.G., Talbot, H. (1981) Photosynthesis as related to xylem water potential and carbon dioxide concentration in Sitka spruce. Physiol. Plant. 52, 391–400

    Google Scholar 

  • Boyer, J.S. (1971) Nonstomatal inhibition of photosynthesis in sunflower at low leaf water potentials and high light intensities. Plant Physiol. 48, 532–536

    Google Scholar 

  • Bradford, K.J., Hsiao, T.C. (1982) Physiological responses to moderate water stress. In: Encyclopedia of plant physiology, N.S., vol. XIIB: Physiological plant ecology, pt. 2: Water relations and carbon assimilation, Lange, O.L., Nobel, P.S., Osmond, C.B., eds. Springer, Berlin Heidelberg New York (in press)

    Google Scholar 

  • Briantais, J.M., Vernotte, C., Picaud, M., Krause, G.H. (1979) A quantitative study of the slow decline of chlorophyll a fluorescence in isolated chloroplasts. Biochim. Biophys. Acta 548, 128–138

    Google Scholar 

  • Caemmerer, S. von (1981) On the relationship between chloroplast biochemistry and gas exchange of leaves. Ph.D. thesis, Australian National University, Canberra

    Google Scholar 

  • Carpita, N., Sabularse, D., Montezinos, D., Delmer, D.P. (1979) Determination of the pore size of cell walls of living plant cells. Science 205, 1144–1147

    Google Scholar 

  • Farquhar, G.D., Sharkey, T.D. (1982) Stomatal conductance and photosynthesis. Annu. Rev. Plant Physiol. 33, 317–345

    Google Scholar 

  • Farquhar, G.D., Caemmerer, S. von, Berry, J.A. (1980) A biochemical model of photosynthetic C2 assimilation in leaves of C3 species. Planta 149, 78–90

    Google Scholar 

  • Gaff, D.F., Okong'O-ogola, O. (1971) The use of non-permeating pigments for testing the survival of cells. J. Exp. Bot. 22, 756–758

    Google Scholar 

  • Govindjee, Downton, W.J.S., Fork, D.C., Armond, P.A. (1981) Chlorophyll a fluorescence transient as an indicator of water potential of leaves. Plant Sci. Lett. 20, 191–194

    Google Scholar 

  • Huffaker, R.C., Radin, T., Kleinkopf, G.E., Cox, E.L. (1970) Effects of mild water stress on enzymes of nitrate assimilation and of the carboxylative phase of photosynthesis in barley. Crop Sci. 10, 471–474

    Google Scholar 

  • Johnson, R.R., Frey, N.M., Moss, D.N. (1974) Effect of water stress on photosynthesis and transpiration of flag leaves and spikes of barley and wheat. Crop Sci. 14, 728–731

    Google Scholar 

  • Jones, H.G. (1973) Moderate-term water stresses and associated changes in some photosynthetic parameters in cotton. New Phytol. 72, 1095–1105

    Google Scholar 

  • Kaiser, W.M., Heber, U. (1981) Photosynthesis under osmotic stress effect of high solute concentrations on the permeability roperties of the chloroplast envelope and on activity of stroma enzymes. Planta 153, 423–429

    Google Scholar 

  • Kaiser, W.M., Kaiser, G., Prachaub, P.K., Wildman, S.G., Heber, U. (1981) Photosynthesis under osmotic stress inhibition of photosynthesis of intact chloroplasts, protoplasts, and leaf slices at high osmotic potentials. Planta 153, 416–422

    Google Scholar 

  • Keck, R.W., Boyer, J.S. (1974) Chloroplast response to low leaf water potentials. III. Differing inhibition of electron transport and photophosphorylation. Plant Physiol. 53, 474–479

    Google Scholar 

  • Lorimer, G.H., Badger, M.R., Andrews, T.J. (1976) The activation of ribulose-1,5-bisphosphate carboxylase by carbon dioxide and magnesium ions. Equilibria, kinetics, a suggested mechanism, and physiological implications. Biochemistry 15, 529–536

    Google Scholar 

  • Lowry, O.H., Passoneau, J.V. (1972) A flexible system of enzymic analysis. Academic Press, New York London

    Google Scholar 

  • Mohanty, P., Boyer, J.S. (1976) Chloroplast response to low leaf water potentials. IV. Quantum yield is reduced. Plant Physiol. 57, 704–709

    Google Scholar 

  • Moldau, H. (1973) Effects of various water regimes on stomatal and mesophyll conductances of bean leaves. Photosynthetica 7, 1–7

    Google Scholar 

  • O'Toole, J.C., Ozbun, J.L., Wallace, D.H. (1977) Photosynthetic response to water stress in Phaseolus vulgaris. Physiol. Plant. 40, 111–114

    Google Scholar 

  • Sharkey, T.D., Raschke, K. (1980) Effects of phaseic acid and dihydrophaseic acid and stomata and the photosynthetic apparatus. Plant Physiol. 65, 291–297

    Google Scholar 

  • Sharkey, T.D., Stevenson, G.F., Paton, D.M. (1982) Effects of G, a growth regulator from Eucalyptus grandis, on photosynthesis. Plant Physiol. 69, 935–938

    Google Scholar 

  • Troughton, J.H., Slatyer, R.O. (1969) Plant water status, leaf temperature, and the calculated mesophyll resistance to carbon dioxide of cotton leaves. Aust. J. Biol. Sci. 22, 815–827

    Google Scholar 

  • Tyree, M.T. (1976) Negative turgor pressure in plant cells: fact or fallacy. Can. J. Bot. 54, 2738–2746

    Google Scholar 

  • Wirtz, W., Stitt, M., Heldt, H.W. (1980) Enzymic determination of metabolites in the subcellular compartments of spinach protoplasts. Plant Physiol. 66, 187–193

    Google Scholar 

  • Younis, H.M., Boyer, J.S., Govindjee (1979) Conformation and activity of chloroplast coupling factor exposed to low chemical potential of water in cells. Biochim. Biophys. Acta 548, 328–340

    Google Scholar 

  • Zimmermann, M.H., Brown, C.L. (1971) Trees. Structure and function. Springer, New York

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sharkey, T.D., Badger, M.R. Effects of water stress on photosynthetic electron transport, photophosphorylation, and metabolite levels of Xanthium strumarium mesophyll cells. Planta 156, 199–206 (1982). https://doi.org/10.1007/BF00393725

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00393725

Key words

Navigation