Abstract
Several component processes of photosynthesis were measured in osmotically stressed mesophyll cells of Xanthium strumarium L. The ribulose-1,5-bisphosphate regeneration capacity was reduced by water stress. Photophoshorylation was sensitive to water stress but photosynthetic electron transport was unaffected by water potentials down to-40 bar (-4 MPa). The concentrations of several intermediates of the photosynthetic carbon-reduction cycle remained relatively constant and did not indicate that ATP supply was limiting photosynthesis in the water-stressed cells.
Similar content being viewed by others
Abbreviations
- Hepes:
-
4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid
- PGA:
-
3-phosphoglyceric acid
- RuBP:
-
ribulose-1,5-bisphosphate
References
Arnon, D.I. (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24, 1–15
Beadle, C.I., Nielson, R.E., Jarvis, P.G., Talbot, H. (1981) Photosynthesis as related to xylem water potential and carbon dioxide concentration in Sitka spruce. Physiol. Plant. 52, 391–400
Boyer, J.S. (1971) Nonstomatal inhibition of photosynthesis in sunflower at low leaf water potentials and high light intensities. Plant Physiol. 48, 532–536
Bradford, K.J., Hsiao, T.C. (1982) Physiological responses to moderate water stress. In: Encyclopedia of plant physiology, N.S., vol. XIIB: Physiological plant ecology, pt. 2: Water relations and carbon assimilation, Lange, O.L., Nobel, P.S., Osmond, C.B., eds. Springer, Berlin Heidelberg New York (in press)
Briantais, J.M., Vernotte, C., Picaud, M., Krause, G.H. (1979) A quantitative study of the slow decline of chlorophyll a fluorescence in isolated chloroplasts. Biochim. Biophys. Acta 548, 128–138
Caemmerer, S. von (1981) On the relationship between chloroplast biochemistry and gas exchange of leaves. Ph.D. thesis, Australian National University, Canberra
Carpita, N., Sabularse, D., Montezinos, D., Delmer, D.P. (1979) Determination of the pore size of cell walls of living plant cells. Science 205, 1144–1147
Farquhar, G.D., Sharkey, T.D. (1982) Stomatal conductance and photosynthesis. Annu. Rev. Plant Physiol. 33, 317–345
Farquhar, G.D., Caemmerer, S. von, Berry, J.A. (1980) A biochemical model of photosynthetic C2 assimilation in leaves of C3 species. Planta 149, 78–90
Gaff, D.F., Okong'O-ogola, O. (1971) The use of non-permeating pigments for testing the survival of cells. J. Exp. Bot. 22, 756–758
Govindjee, Downton, W.J.S., Fork, D.C., Armond, P.A. (1981) Chlorophyll a fluorescence transient as an indicator of water potential of leaves. Plant Sci. Lett. 20, 191–194
Huffaker, R.C., Radin, T., Kleinkopf, G.E., Cox, E.L. (1970) Effects of mild water stress on enzymes of nitrate assimilation and of the carboxylative phase of photosynthesis in barley. Crop Sci. 10, 471–474
Johnson, R.R., Frey, N.M., Moss, D.N. (1974) Effect of water stress on photosynthesis and transpiration of flag leaves and spikes of barley and wheat. Crop Sci. 14, 728–731
Jones, H.G. (1973) Moderate-term water stresses and associated changes in some photosynthetic parameters in cotton. New Phytol. 72, 1095–1105
Kaiser, W.M., Heber, U. (1981) Photosynthesis under osmotic stress effect of high solute concentrations on the permeability roperties of the chloroplast envelope and on activity of stroma enzymes. Planta 153, 423–429
Kaiser, W.M., Kaiser, G., Prachaub, P.K., Wildman, S.G., Heber, U. (1981) Photosynthesis under osmotic stress inhibition of photosynthesis of intact chloroplasts, protoplasts, and leaf slices at high osmotic potentials. Planta 153, 416–422
Keck, R.W., Boyer, J.S. (1974) Chloroplast response to low leaf water potentials. III. Differing inhibition of electron transport and photophosphorylation. Plant Physiol. 53, 474–479
Lorimer, G.H., Badger, M.R., Andrews, T.J. (1976) The activation of ribulose-1,5-bisphosphate carboxylase by carbon dioxide and magnesium ions. Equilibria, kinetics, a suggested mechanism, and physiological implications. Biochemistry 15, 529–536
Lowry, O.H., Passoneau, J.V. (1972) A flexible system of enzymic analysis. Academic Press, New York London
Mohanty, P., Boyer, J.S. (1976) Chloroplast response to low leaf water potentials. IV. Quantum yield is reduced. Plant Physiol. 57, 704–709
Moldau, H. (1973) Effects of various water regimes on stomatal and mesophyll conductances of bean leaves. Photosynthetica 7, 1–7
O'Toole, J.C., Ozbun, J.L., Wallace, D.H. (1977) Photosynthetic response to water stress in Phaseolus vulgaris. Physiol. Plant. 40, 111–114
Sharkey, T.D., Raschke, K. (1980) Effects of phaseic acid and dihydrophaseic acid and stomata and the photosynthetic apparatus. Plant Physiol. 65, 291–297
Sharkey, T.D., Stevenson, G.F., Paton, D.M. (1982) Effects of G, a growth regulator from Eucalyptus grandis, on photosynthesis. Plant Physiol. 69, 935–938
Troughton, J.H., Slatyer, R.O. (1969) Plant water status, leaf temperature, and the calculated mesophyll resistance to carbon dioxide of cotton leaves. Aust. J. Biol. Sci. 22, 815–827
Tyree, M.T. (1976) Negative turgor pressure in plant cells: fact or fallacy. Can. J. Bot. 54, 2738–2746
Wirtz, W., Stitt, M., Heldt, H.W. (1980) Enzymic determination of metabolites in the subcellular compartments of spinach protoplasts. Plant Physiol. 66, 187–193
Younis, H.M., Boyer, J.S., Govindjee (1979) Conformation and activity of chloroplast coupling factor exposed to low chemical potential of water in cells. Biochim. Biophys. Acta 548, 328–340
Zimmermann, M.H., Brown, C.L. (1971) Trees. Structure and function. Springer, New York
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sharkey, T.D., Badger, M.R. Effects of water stress on photosynthetic electron transport, photophosphorylation, and metabolite levels of Xanthium strumarium mesophyll cells. Planta 156, 199–206 (1982). https://doi.org/10.1007/BF00393725
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00393725