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Transposon donor plasmids, based on ColIb-P9, for use in Pseudomonas putida and a variety of other Gram negative bacteria

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Summary

The properties of pLG221, a derivative of the ColIb plasmid carrying the transposon Tn5 are described. This plasmid can be used to introduce Tn5 by conjugation from Escherichia coli into a variety of Gram negative bacteria outside the host range for maintenance of ColIb. Plasmid pLG221, and a similar plasmid pLG223 carrying Tn10 may be of general utility as vectors for transposon-mediated mutagenesis in a variety of Gram negative bacteria.

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Author notes

  1. Jennifer M. Varley

    Present address: University/ICI Joint Laboratory, University of Leicester, University Road, LE1 7RH, Leicester, UK

  2. Graham J. Boulnois

    Present address: Department of Microbiology, University of Leicester, University Road, LE1 7RH, Leicester, UK

  3. F. Christopher H. Franklin

    Present address: Department of Genetics, University of Birmingham, Birmingham, UK

Authors and Affiliations

  1. Department of Medical Biochemistry, University Medical Centre, 9, Avenue de Champel, CH-1211, Geneva 4, Switzerland

    Graham J. Boulnois, Jennifer M. Varley & F. Christopher H. Franklin

  2. University/ICI Joint Laboratory, University of Leicester, University Road, LE1 7RH, Leicester, UK

    Geoffrey S. Sharpe

Authors
  1. Graham J. Boulnois
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  2. Jennifer M. Varley
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  3. Geoffrey S. Sharpe
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  4. F. Christopher H. Franklin
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Additional information

Communicated by P.T. Emmerson

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Boulnois, G.J., Varley, J.M., Sharpe, G.S. et al. Transposon donor plasmids, based on ColIb-P9, for use in Pseudomonas putida and a variety of other Gram negative bacteria. Molec. Gen. Genet. 200, 65–67 (1985). https://doi.org/10.1007/BF00383313

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  • DOI: https://doi.org/10.1007/BF00383313

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