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The influence of ozone and nutrition on δ13C in Betula pendula

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Abstract

In the cellulose of stems and leaves, δ13C was investigated in a birch clone (Betula pendula), which was exposed throughout the growing season to either <3 (control) or 90/40 nl O3 1-1 (day/night). Each regime was split into plants under high or low nutrient supply. δ13C was increased (becoming less negative), in stems rather than leaves, by both high nutrition (+2‰) and O3 stress (+1‰). Whereas high nutrition raised the wateruse efficiency (WUE) while lowering the CO2 concentration in the inner leaf air space (c i), WUE decreased and c i increased under O3 stress. Therefore, only the nutritional effect on the carbon isotope fractionation was reproduced by the model of Farquhar et al. (1982) which estimates WUE by means of δ13C based on c i. c i was not biased by ‘patchiness’ in respect to stomatal opening. The latter was verified by microscopical analysis and the complete water infiltration of the birch leaves through the stomata, independent of the diurnal course of the leaf conductance for water vapour. Under low nutrient supply, the activity of phosphoenol pyruvate carboxylase (PEPC) was roughly doubled by ozone to about 1.3% of the total carboxylation capacity (by PEPC + rubisco), and was increased to 1.7% under high nutrition. The fractionation model, extended to account for varying activities of the carboxylating enzymes, indicated that stimulated PEPC was the cause of elevated δ13C, although c i was increased under O3 stress. The stimulation of PEPC and, as a consequence, elevated δ13C are discussed as part of a whole-plant acclimation to O3 stress.

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Author notes

  1. Rainer Matyssek

    Present address: Department of Forest Botany, University of Munich, Hohenbachernstr. 22, D-85354, Freising, Germany

Authors and Affiliations

  1. Swiss Federal Institute for Forest, Snow and Landscape Research, Zürcherstrasee 111, CH-8903, Birmensdorf ZH, Switzerland

    Stefan Maurer, Rainer Matyssek, Werner Landolt & Madeleine S. Günthardt-Goerg

  2. Paul Scherrer Institute, CH-5232, Villigen, Switzerland

    Matthias Saurer

  3. Physics Institute, University of Berne, Sidlerstr. 5, CH-3012, Berne, Switzerland

    Ulrich Siegenthaler

Authors
  1. Matthias Saurer
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Saurer, M., Maurer, S., Matyssek, R. et al. The influence of ozone and nutrition on δ13C in Betula pendula . Oecologia 103, 397–406 (1995). https://doi.org/10.1007/BF00328677

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