The acquisition of inorganic carbon by four red macroalgae

Abstract

Photosynthesis was studied in four species of red marine macroalgae: Palmaria palmata, Laurencia pinnatifida, Lomentaria articulata and Delesseria sanguinea. The rate of O₂ evolution for submersed photosynthesis was measured as a function of incident photon flux density at normal pH and inorganic carbon concentration (pH 8.0, 2 mol m⁻³), and as a function of inorganic carbon concentration at pH 8.0 at saturating and at limiting photon flux density. The rate of CO₂ uptake was measured for emersed photosynthesis as a function of CO₂ partial pressure at saturating photon flux density. Previous pH-drift results suggest that Palmaria and Laurencia are able to use HCO ^sup−_inf3 as well as CO₂ whereas Lomentaria and Delesseria are restricted to CO₂. None of the algae are saturated by 2 mol m⁻³ inorganic carbon at high light (400 μmol m⁻² s⁻¹) but are saturated at low light (35 μmol m⁻² s⁻¹). The inorganic C concentration at which half the light-saturated rate of O₂ evolution is achieved is higher for Palmaria and Laurencia (1.51 and 1.85 mol m⁻³) than for Lomentaria and Delesseria (0.772 and 0.841 mol m⁻³). The lower values for the latter two species could reflect their putative restriction to CO₂. If expressed in terms of CO₂, the half-saturation values yield 7.2 and 7.8 mmol m⁻³ respectively, which are very similar to values obtained previously during pH-drift experiments but at lower concentrations of HCO ^sup−_inf3 , consistent with restriction to CO₂. The photosynthetic conductance (m s⁻¹), calculated from the initial slope for photosynthesis at low concentrations of inorganic carbon, correlates with the suggested ability to extract inorganic carbon based on pH-drift results. Calculations made assuming that CO₂ is the only species diffusing across the boundary layer are consistent with boundary layer thicknesses of 20 and 19 μm for Lomentaria and Delesseria respectively, which is feasible given the rapid water movement in the experiments. For Laurencia however, an unreasonably small boundary layer thickness of 6 μm is necessary to explain the flux, which indicates co-diffusion by HCO ^sup−_inf3 . In the apparent absence of external carbonic anhydrase, direct uptake of HCO ^sup−_inf3 , rather than external conversion to CO₂ is indicated in this species. In air, the CO₂ concentration at which photosynthesis is half-maximal increases in the same order as the ability to raise pH in drift experiments. At 21 kPa the CO₂ compensation partial pressures for Palmaria and Laurencia at 0.56 and 1.3 Pa are low enough to suggest a carbon-concentrating mechanism is operating, while those of Lomentaria at 1.8 Pa and particularly that of Delesseria at 4.5 Pa could be explained without a carbon-concentrating mechanism. The algae tested (all except Delesseria) showed more O₂ evolution than could be accounted for with a photosynthetic quotient of 1.0 and uncatalysed conversion of HCO ^sup−_inf3 to CO₂ outside the cell in high light at pH 8.0 when high algal fresh weight per unit medium was used. These results are concordant with other data suggesting use of HCO ^sup−_inf3 by Palmaria and Laurencia, but discordant with the rest of the available information in indicating use of HCO ^sup−_inf3 by Lomentaria. The reason for this is unclear. The lightsaturated rate of O₂ evolution on an algal area basis and the photon flux density needed to saturate photosynthesis were related partly to the habitat from which the seaweeds were collected, but more strongly to the ability to use HCO ^sup−_inf3 . Values for the two users of HCO ^sup−_inf3 , Palmaria (population used was intertidal; also occurs subtidally) and Laurencia (intertidal/shaded intertidal), were greater than for Lomentaria (shaded intertidal), which was greater than Delesseria (subtidal), both of which are believed to be restricted to CO₂. In accordance with earlier δ¹³C data and, for Delesseria, estimates of the achieved growth rates in situ, carbon is likely to be saturating and use of HCO ^sup−_inf3 is unlikely to occur in the normal low-light habitats of Lomentaria and Delesseria. Analysis of N-use efficiencies show that they are closer to the low-CO₂-affinity Laminariales than the high-CO₂-affinity Fucaceae.