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Hyper-recombination in dam mutants of Escherichia coli K-12

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Summary

F-prime heterogenotes of dam-3 bacteria segregate F-prime homogenotes at a frequency 30–200 times higher than the isogenic dam + strain. A hyperrecombination mutant which shows increased recombination between chromosomal duplications was characterized as a dam mutant. The dam-3 allele causes a reduction in linkage of proximal unselected markers in transconjugants and increases the recombination frequency between a pair of closely linked markers. It is concluded that dam mutations confer a hyperrecombination phenotype to the cell.

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Authors and Affiliations

  1. Dept. of Pharmacology, University of Massachusetts Medical School, 01605, Worcester, MA

    M. G. Marinus

  2. Dept. of Biochemical Pharmacology, N.I.A.M.D.D., National Institutes of Health, 20014, Bethesda, MD

    E. Bruce Konrad

Authors
  1. M. G. Marinus
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  2. E. Bruce Konrad
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Communicated by B.A. Bridges

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Marinus, M.G., Konrad, E.B. Hyper-recombination in dam mutants of Escherichia coli K-12. Molec. Gen. Genet. 149, 273–277 (1976). https://doi.org/10.1007/BF00268528

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  • DOI: https://doi.org/10.1007/BF00268528

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