Abstract
Intertribal somatic hybrids were produced between Brassica napus and Barbarea vulgaris. The two species belong to the tribes Arabidae and Brassiceae, respectively, B. vulgaris is known to be cold tolerant and of interest to use as a gene donor to rapeseed. Of the plants produced in five fusion experiments six plants were verified to be hybrids by RFLP analysis utilizing one B. vulgaris-specific repetitive DNA sequence and two nuclear probes (rDNA and cruciferin) as markers. When analysing nuclear DNA content in four of these six hybrids, all had a higher DNA content than B. napus. However, mature plants could not be established outside in vitro conditions, indicating problems with compatibility between the species.
Similar content being viewed by others
References
Bauer-Weston BJ (1990) M.Sc Thesis, Agriculture Canada, Ottawa, Ontario.
Bernatzky R and Tanksley SD (1986) Theor Appl Genet 72:314–321
de Vries SE, Ferwerda MA, Loonen AEHM, Pijnacker LP, Feenstra WJ (1987) Theor Appl Genet 75:170–176
Fahleson J, Dixelius J, Sundberg E, Glimelius K (1988) Plant Cell Reports 7:74–77
Fahleson J, Eriksson I, Landgren M, Stymne S, Glimelius K (1994) Theor Appl Genet (in press)
Forsberg J, Landgren M, Glimelius K (1994) Fertile somatic hybrids between Brassica napus and Arabidopsis thaliana. Plant Science (in press)
Gleba YY, Hoffmann F (1980) Planta 149:112–117
Glimelius K (1984) Physiol Plant 61:38–44
Glimelius K, Fahleson J, Landgren M, Sjödin C, Sundberg E (1991) Tibtech 9:24–30
Gupta PP, Schieder O, Gupta M (1984) Mol Gen Genet 197:30–35
Handley LW, Nickels RL, Cameron MW, Moore PP, Sink KC (1986) Theor Appl Genet 71:691–697
Helgeson JP, Haberlach GT, Pohlman J, Austin S (1987) In: Puite KJ, Dons JJM, Huizing HJ, Kool AJ, Koornneef M, Krens FA (eds). Progress in plant protoplast research, Proceedings of the 7th international protoplast symposium, Wageningen, the Netherlands, December 6–11, 1987, pp 205–207
Hinnisdaels S, Negrutiu I, Jacobs M, Sidorov V (1988) IAPTC Newsletter 55:2–10
Hoffman F, Adachi T (1981) Planta 153:586–593
Imamura J, Saul MW, Potrykus I (1987) Theor Appl Genet 74:445–450
Klimaszewska K, Keller WA (1988) Plant Sci 56:61–68
Landgren M, Glimelius K (1990) Theor Appl Genet 80:776–784
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory
Menczel L, Nagy F, Kiss ZR, Maliga P (1981) Theor Appl Genet 59:191–195
Murashige T and Skoog, F (1962) Physiol. Plant 15:473–497
Oikarinen S, Ryöppy PH (1992) Abstract at the XIIIth EUCARPIA Congress, Angers, France July 06–11th 1992. 261–262
Puite KJ (1991) Progress in plant protoplast research. Physiol Plant 85:403–410
Rödin J, Ericson ML, Josefsson LG, Rask L (1990) The J of Biological Chemistry 265:2720–2723
Sacristán MD, Gerdemann-Knörck M, Schieder O (1989) Theor Appl Genet 78:194–200
Schweizer G, Ganal M, Ninnemann H, Hemleben V (1988) Theor Appl Genet 75:679–684
Sjödin C, Glimelius K (1989) Theor Appl Genet 77:651–656
Sundberg E, Glimelius K (1986) Plant Sci 43:155–162
Sundberg E, Glimelius K (1991) Theor Appl Genet 83:81–88
Sundberg E, Lagercrantz U, Glimelius K (1991) Plant Science 78:89–98
Toki S, Kameya T, Abe T (1990) Theor Appl Genet 80:588–592
Yakura Y, Atsushi K, Tanifuji S (1984) Mol Gen Genet 193:400–405
Author information
Authors and Affiliations
Additional information
Communicated by M. R. Davey
Rights and permissions
About this article
Cite this article
Fahleson, J., Eriksson, I. & Glimelius, K. Intertribal somatic hybrids between Brassica napus and Barbarea vulgaris — production of in vitro plantlets. Plant Cell Reports 13, 411–416 (1994). https://doi.org/10.1007/BF00234149
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00234149