Summary
Biochemical and genetical characterization of a rice nitrate reductase (NR)-deficient mutant, M819, which had been isolated as a chlorate-resistant mutant, was carried out. In M819, leaf NADH-NR activity was found to be about 10% of that of the wild-type cv ‘Norin 8’, while NADPH-NR activity was higher than that in the wild-type; FMNH2-NR and MV-NR activities were also 10% of those of the wild type; BPB-NR activity was higher than that of the wild type; and xanthine dehydrogenase activity was revealed to be present in both. These results suggest that the mutant line M819 lacks the functional heme domain of the NADH-NR polypeptide due to a point mutation or a small deletion within the coding region of the structural gene. Chlorate resistance in M819 was transmitted by a single recessive nuclear gene.
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Abbreviations
- NR:
-
Nitrate reductase
- NiR:
-
nitrite reductase
- FMNH2 :
-
reduced flavin nucleotide
- MV:
-
reduced methyl viologen
- BPB:
-
reduced bromphenol blue
- XDH:
-
xanthine dehydrogenase
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Communicated by H. F. Linskens
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Hasegawa, H., Katagiri, T., Ida, S. et al. Characterization of a rice (Oryza sativa L.) mutant deficient in the heme domain of nitrate reductase. Theoret. Appl. Genetics 84, 6–9 (1992). https://doi.org/10.1007/BF00223974
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DOI: https://doi.org/10.1007/BF00223974