Abstract
The photo-induced toxicity of the polycyclic aromatic hydrocarbon (PAH), anthracene, to the green alga, Selenastrum capricornutum, was characterized. The dose-response relationships among anthracene concentration, ultraviolet (UV) radiation intensity, and algal growth rate and 14C-bicarbonate incorporation were determined. The 22 h EC50 for specific growth rate was inversely related to UV-A radiation intensity and ranged from 37.4 to 3.9 μg/L anthracene. For 14C-bicarbonate incorporation on a volume basis (primary production), the 24 h EC50 ranged from 24.0 to 3.3 μg/L anthracene depending on the UV-A intensity. The incorporation of 14C-bicarbonate on a per cell basis (cellular photosynthesis) was more resistant than cell growth or primary production. The threshold for photo-induced toxicity of anthracene was 1.5–3 μg/L anthracene, however, no UV-A radiation threshold was evident for many of the measurements of toxicity studied. Algae appeared to be slightly more resistant to photo-induced toxicity of anthracene than fishes and invertebrates. An environmental hazard assessment suggests that some aquatic systems are sufficiently contaminated by PAH that a hazard to natural algal communities due to photo-induced toxicity of PAH may be present.
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References
Allred PM, Giesy JP (1985) Solar radiation-induced toxicity of anthracene to Daphnia pulex. Environ Toxicol Chem 4:219–226
Andelman JB, Suess MJ (1970) Polynuclear aromatic hydrocarbons in the water environment. Bull Wld Hlth Org 43:479–508
Anon (1981) OECD guidelines for testing of chemicals: 201. Alga growth inhibition test. Organization for Economic Cooperation and Development, Paris, France
Barrick RC, Prahl FG (1987) Hydrocarbon geochemistry of the Puget Sound region - III. Polycyclic aromatic hydrocarbons in sediments. Estuar Coastal Shelf Sci 25:175–191
Bowling JW, Leversee GJ, Landrum PF, Giesy JP (1983) Acute mortality of anthracene-contaminated fish exposed to sunlight. Aquat Toxicol 3:79–90
Brandle JR, Campbell WF, Sisson WB, Caldwell MM (1977) Net photosynthesis, electron transport capacity, and ultrastructure of Pisum sativum L. exposed to ultraviolet-B radiation. Plant Physiol 60:165–169
Cody TE, Radike MJ, Warshawsky D (1984) The phototoxicity of benzo[a]pyrene in the green alga Selenastrum capricornutum. Environ Res 35:122–132
Davenport R, Spacie S (1991) Acute phototoxicity of harbor and tributary sediments from lower Lake Michigan. J Great Lakes Res 17:51–56
Eadie BJ, Faust WR, Landrum PF, Morehead NR, Gardner WS, Napela T (1983) Bioconcentration of PAH by some benthic organisms. In: Cooke M, Dennis J (eds) Polynuclear aromatic hydrocarbons: Formation, metabolism and measurement, 7th International Symposium. Battelle Press, Columbus, OH, pp 437–449
Eisler R (1987) Polycyclic aromatic hydrocarbon hazards to fish, wildlife, and invertebrates: a synoptic review. US Fish Wildl Serv Biol Rep 85 (1.11). US Fish and Wildlife Service, Laurel, MD
Fabacher DL, Schmitt CJ, Besser JM, Mac MJ (1988) Chemical characterization and mutagenic properties of polycyclic aromatic compounds in sediment from tributaries of the Great Lakes. Environ Toxicol Chem 7:529–543
Finney DJ (1971) Probit analysis, 3rd ed. Cambridge University Press, London
Futoma DJ, Smity SR, Smith TE, Tanaka J (1981) Polycyclic aromatic hydrocarbons in water systems. CRC Press, Boca Raton, FL, pp 25–27
Gala WR, Giesy JP (1991) Effects of ultraviolet radiation on the primary production of natural phytoplankton assemblages in Lake Michigan. Ecotoxicol Environ Safety 22:345–361
Giesy JP, Bartell SM, Landrum PF, Leversee GJ, Bowling JW, Bruno MG, Fannin TE, Gerould S, Haddock JD, LaGory K, Oris JT, Spacie A (1978) Fates and biological effects of polycyclic aromatic hydrocarbons in aquatic systems. EPA-78-D-X0290, US Environmental Protection Agency, Athens, GA
Griest WH (1980) Multicomponent polycyclic aromatic hydrocarbon analysis of inland water and sediment. In: Afgan BK, Mackay D (eds) Hydrocarbons and halogenated hydrocarbons in the aquatic environment. Plenum Press, New York, pp 173–183
Holst LL, Giesy JP (1989) Chronic effects of the photoenhanced toxicity of anthracene on Daphnia magna reproduction. Environ Toxicol Chem 8:933–942
Jokiel PL, York RH (1984) Importance of ultraviolet radiation in photoinhibition of microalgal growth. Limnol Oceanogr 29:192–199
Kaidbey KH, Nonaka S (1984) Action spectrum for anthracene-induced photosensitization of human skin. Photochem Photobiol 39:375–378
Landrum PF, Reinhold MD, Nihart SR, Eadie BJ (1985) Predicting the bioavailability of organic xenobiotics to Pontoporeia hoyi in the presence of humic and fulvic materials and natural dissolved organic matter. Environ Toxicol Chem 4:459–467
Leversee GJ, Landrum PF, Giesy JP, Fannin T (1983) Humic acids reduce bioaccumulation of some polycyclic aromatic hydrocarbons. Can J Fish Aquat Sci 40 (Suppl):63–69
Lorenzen CJ (1979) Ultraviolet radiation and phytoplankton photosynthesis. Limnol Oceanogr 24:1117–1120
Mailhot H (1987) Prediction of algal bioaccumulation and uptake rate of nine organic compounds by ten physiochemical properties. Environ Sci Technol 27:1009–1013
Mill T, Mabey WR, Bomberger DC, Chou T-W, Hendry DG, Smith JH (1982) Laboratory protocols for evaluating the fate of organic chemicals in air and water. EPA 600/3–82–022, U.S. Environmental Protection Agency, Athens, GA
Miller WE, Greene JC, Shiroyama T (1978) The Selenastrum capricornutum Printz algal assay bottle test. EPA 660/9–78–018, U.S. Environmental Protection Agency, Corvallis, OR
Neff JM (1979) Polycyclic aromatic hydrocarbons in the aquatic environment. Applied Science, London
Neter J, Wasserman W, Kutner MH (1985) Applied Linear Statistical Models, 2nd ed. Richard D Irwin, Homewood, IL, pp 171–174
Newsted JL, Giesy JP (1987) Predictive model for photoinduced acute toxicity of polycyclic aromatic hydrocarbons to Daphnia magna, Strauss (Cladocera, Crustacea). Environ Toxicol Chem 6:445–461
Oris JT, Giesy JP (1985) The photoenhanced toxicity of anthracene to juvenile sunfish (Lepomis spp.) Aquat Toxicol 6:133–146
—, — (1986) Photoinduced toxicity of anthracene to juvenile bluegill sunfish (Leopmis macrochirus Rafinesque): Photoperiod effects and predictive hazard evaluation. Environ Toxicol Chem 5:761–768
—, — (1987) The photo-induced toxicity of selected polycyclic aromatic hydrocarbons to larvae of the fathead minnow (Pimephales promelas): comparative toxicities and structure-activity relationship. Chemosphere 16:1395–1404
Oris JT, Giesy JP, Allred PM, Grant DF, Landrum PF (1984) Photoinduced toxicity of anthracene in aquatic organisms: An environmental perspective. In: Veziroglu TN (ed) The biosphere: Problems and solutions. Elsevier Science Publ B.V., Amsterdam, pp. 639–658
Readman JW, Mantoura RFC, Rhead MM (1987) A record of polycyclic aromatic hydrocarbon (PAH) pollution obtained from accreting sediments of the Tamar Estuary, U.K.: Evidence for nonequilibrium behaviour of PAH. Sci Total Environ 66:73–94
SAS Institute (1987) SAS/STAT guide for personal computers, Version 6 edition. SAS Institute Inc., Cary, NC
Schindler DW, Schmidt RV, Reid RA (1972) Acidification and bubbling as an alternative to filtration in determining phytoplankton production by the 14C method. J Fish Res Board Can 29:1627–1631
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Gala, W.R., Giesy, J.P. Photo-induced toxicity of anthracene to the green alga, Selenastrum capricornutum . Arch. Environ. Contam. Toxicol. 23, 316–323 (1992). https://doi.org/10.1007/BF00216240
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DOI: https://doi.org/10.1007/BF00216240