Abstract
A protein which binds specifically to [3H]-zeatin has been isolated from cucumber cotyledons by chromatographic techniques. Its binding to [3H]-zeatin was inhibited remarkably by the addition of non-radioactive cytokinins and the order of inhibition was zeatin > -zeatin riboside > N6-(Δ2-isopentenyl)adenine > N6-(Δ2-isopentenyl)adenosine > N6-benzyl-adenosine > kinetin riboside. This protein behaved as a soluble protein with an apparent molecular size of 43,000 daltons on gel filtration through calibrated Sephadex G-100 column. The dissociation constant, Kd, of the protein-zeatin complex was about 4 × 10−7 M.
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References
Armstrong, DJ, Burrows, WJ, Evans, PK andSkoog, F (1969) Isolation of cytokinins from tRNA. Biochem Biophys Res Commun 37: 451–456
Berridge, MV, Ralph, RK and Letham, DS (1970) The binding of kinetin to ribosomes. Biochem J 119: 75–84
Chen, CM, Melitz, DK, Petschow, B and Eckert, RL (1980) Isolation of cytokinin binding protein from plant tissues by affinity chromatography. Eur J Biochem 108: 379–387
Erion, JL and Fox, JE (1981) Purification and properties of a protein which binds cytokinin-active 6-substituted purines. Plant Physiol 67: 156–162
Fox, JE and Erion, JL (1975) A cytokinin binding protein from higher plant ribosomes. Biochem Biophys Res Commun 64: 695–700
Keim, P, Erion, J and Fox, JE (1981) The current status of cytokinin-binding moieties. In: JGuern and CPéaud-Lenoël eds. Metabolism and Molecular Activities of Cytokinins, 179–190. Berlin, Heidelberg, New York: Springer-Verlag
Lowry, OH, Rosebrough, NJ, Farr, AL and Randall, RJ (1951) Protein measurement with the folin phenol reagent. J. Biol Chem 193: 265–275
Matthysse, AG and Abrams, M (1970) A factor mediating interaction of kinins with the genetic material. Biochim Biophys Acta 199: 511–518
Moore, FH (1979) A cytokinin-binding protein from wheat germ. Plant Physiol 64: 594–599
Polya, GM and Davis, AW (1978) Properties of high affinity cytokinin-binding protein from wheat germ. Planta 139: 139–147
Polya, GM and Bowman, JA (1979) Ligand specificity of high affinity cytokinin-binding protein. Plant Physiol 64: 387–392
Ramanov, GA, Taran, VYa, Chvojka, L and Kulaeva, ON (1988) Receptor-like cytokinin-binding proteins from barley leaves. J Plant Growth Regul 7: 1–17
Reddy, ASN, Sopory, SK and Datta, A (1983) Purification and characterization of a cytokinin-binding protein from barley embryos. Biochem Int 6: 181–190
Scatchard, G (1949) The attractions of proteins for small molecules and ions. Ann NY Acad Sci 51: 660–672
Sussman, MR and Kende, H (1978) In vitro cytokinin binding to a particulate fraction of tobacco cells. Planta 140: 251–259
Yoshida, K and Takegami, T (1977) Isolation of cytokinin-binding protein from tobacco leaves by bioaffinity chromatography and its partial characterization. J Biochem 81: 791–799
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Jayabaskaran, C. Isolation and characterization of a cytokinin-binding protein from cucumber cotyledons. Plant Growth Regul 9, 9–17 (1990). https://doi.org/10.1007/BF00025274
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DOI: https://doi.org/10.1007/BF00025274