Abstract
The pattern of sequence organization in the regions of the pea genome near sequences coding for mRNA differs significantly from that in total DNA. Interspersion of repeated and single copy sequences is so extensive that 85% of 1300 nucleotide-long fragments contain highly repetitive sequences (about 5000 copies per haploid genome). However, data presented here demonstrate that sequences which code for mRNA are enriched in the small fraction of fragments which do not contain these highly repetitive sequences. Thus, in contrast to the great majority of other sequences in the genome, most mRNA coding sequences are not located within 1300 nucleotides of highly repetitive elements. Moreover, our data indicate that those repeats (if any) which are closely associated with mRNA coding sequences belong to low copy number families characterized by an unusually low degree of sequence divergence.
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Abbreviations
- NT:
-
nucleotides
- NTP:
-
nucleotide pairs
- Cot:
-
the product of molar concentration of DNA nucleotides and time of incubation (mol s/L)
- Tm :
-
the temperature at which half of the nucleotides are unpaired
- Tm,i :
-
the temperature at which half of the complementary strands are completely separated
- PIPES:
-
1, 4, Piperazinediethane sulfonic acid
- PB:
-
an equimolar mixture of NaH2PO4 and Na2HPO4 (pH 6.8).
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Murray, M.G., Thompson, W.F. Repeat sequence interspersion in coding DNA of peas does not reflect that in total pea DNA. Plant Mol Biol 1, 143–153 (1982). https://doi.org/10.1007/BF00024977
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DOI: https://doi.org/10.1007/BF00024977