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Alcohol dehydrogenase gene expression in potato following elicitor and stress treatment

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Abstract

A cDNA clone corresponding to a mRNA that rapidly accumulates during the hypersensitive-like response induced by elicitor treatment of potato (Solanum tuberosum L.) tuber was characterized. The clone encodes a polypeptide (M r=41097) having 83%–85% amino acid identity with known plant alcohol dehydrogenase sequences (ADH; EC 1.1.1.1). The identity of the clone was confirmed by measuring the ADH enzyme activity in extracts of Escherichia coli transformed with the cDNA clone. In potato tuber disks, a wide range of stresses, including treatment with fatty acid elicitors, salicylic acid, UV light and anaerobiosis, was shown to induce accumulation of Adh transcripts. In stems, a high constitutive level of Adh transcripts could be detected in 4-week old plants, but not in 8-week old plants. However, the mRNA could be induced to accumulate in stems of 8-week old plants by treatment with arachidonic acid elicitor or by anaerobiosis. Induction in leaves was also obtained during anaerobiosis and after treatment with a Phytophthora infestans mycelial homogenate.

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Authors and Affiliations

  1. Department of Biochemistry, Université de Montréal, H3C 3J7, Montréal, Canada

    Daniel P. Matton, Peter Constabel & Normand Brisson

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  1. Daniel P. Matton
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  2. Peter Constabel
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  3. Normand Brisson
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Matton, D.P., Constabel, P. & Brisson, N. Alcohol dehydrogenase gene expression in potato following elicitor and stress treatment. Plant Mol Biol 14, 775–783 (1990). https://doi.org/10.1007/BF00016510

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  • DOI: https://doi.org/10.1007/BF00016510

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