Abstract
Polyspecific antibody to a 17 amino acid synthetic peptide from the maize T-cytoplasm urf13-T mitochondrial open reading frame immunoprecipitated a 13 kD polypeptide from 35S-methionine incorporations of T cytoplasm maize. Male-fertile, toxin-insensitive mutants in which the urf13-T sequence is deleted do not synthesize the 13 kD polypeptide. A mutant designated T-4, which carries a 5 bp insertion and a premature stop codon, synthesizes a truncated polypeptide, corresponding to an open reading frame of 8.3 kD. Thus the 13 kD polypeptide is trunctated or absent in mutants expressing male fertility and toxin insensitivity in T-cytoplasm maize.
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Wise, R.P., Fliss, A.E., Pring, D.R. et al. urf13-T of T cytoplasm maize mitochondria encodes a 13 kD polypeptide. Plant Mol Biol 9, 121–126 (1987). https://doi.org/10.1007/BF00015644
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DOI: https://doi.org/10.1007/BF00015644