Overview
- Nominated as an outstanding Ph.D. thesis by the Tokyo Institute of Technology
- Proposes a novel trace detection concept based on a coupled acoustic-gravitational field
- Analyzes the kinetics and thermodynamics of complex reactions that cannot be evaluated by other methods
- Enables readers to detect various bioanalytical targets by simple modifications of the method
Part of the book series: Springer Theses (Springer Theses)
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Table of contents (7 chapters)
Keywords
About this book
The author proposes novel trace-level biosensing based on measurements of the levitation coordinate shift of an MP in the CAG field. The levitation coordinate of the MP in the CAG field is determined by its density and compressibility. The levitation coordinate shift is induced by the binding of gold nanoparticles (AuNPs) to the MP through interparticle reactions. Therefore, the quantity of molecules involved in the reaction can be determined from the levitation coordinate shift. The author demonstrates the zmol level detection for biotin, DNA/RNA, and organic molecules. In addition, the kinetics and thermodynamics are evaluated for various reactions occurring between the MP and AuNP, such as the avidin-biotin reaction, direct hybridization, sandwich hybridization, and aptamer-target complexation.
This book provides a new concept based on the CAG field, in which the extent of a reaction is converted into the levitation coordinate shift, that is, “length.” The proposed method has many advantages over other methods, e.g., high biocompatibility, high applicability, and short analysis time. In addition, because the apparatus used in this study is inexpensive and easy to miniaturize, this method is useful in important practical fields, such as forensic and environmental science and diagnosis. Thus, this book inspires many researchers to apply the present method to their own fields of interest.
Authors and Affiliations
About the author
Bibliographic Information
Book Title: Acoustic Levitation-Based Trace-Level Biosensing
Book Subtitle: Design of Detection Systems and Applications to Real Samples
Authors: Akihisa Miyagawa
Series Title: Springer Theses
DOI: https://doi.org/10.1007/978-981-16-1425-5
Publisher: Springer Singapore
eBook Packages: Chemistry and Materials Science, Chemistry and Material Science (R0)
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2021
Hardcover ISBN: 978-981-16-1424-8Published: 21 April 2021
Softcover ISBN: 978-981-16-1427-9Published: 22 April 2022
eBook ISBN: 978-981-16-1425-5Published: 20 April 2021
Series ISSN: 2190-5053
Series E-ISSN: 2190-5061
Edition Number: 1
Number of Pages: XII, 93
Number of Illustrations: 18 b/w illustrations, 44 illustrations in colour
Topics: Analytical Chemistry, Physical Chemistry, Biochemistry, general, Measurement Science and Instrumentation