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Resin Microscopy and On-Section Immunocytochemistry

  • Book
  • © 1993

Overview

Authors:
  1. Geoffrey R. Newman

    1. Electron Microscopy Unit, University of Wales College of Medicine, Cardiff, Great Britain

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    You can also search for this author in PubMed   Google Scholar

  2. Jan A. Hobot

    1. Electron Microscopy Unit, University of Wales College of Medicine, Cardiff, Great Britain

    View author publications

    You can also search for this author in PubMed   Google Scholar

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (10 chapters)

  1. Front Matter

    Pages I-XIX
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  2. Resin Embedding

    1. Front Matter

      Pages 1-1
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    2. The Strategic Approach

      • Geoffrey R. Newman, Jan A. Hobot
      Pages 3-25

    3. The Resins

      • Geoffrey R. Newman, Jan A. Hobot
      Pages 27-45

    4. Resin Embedding Protocols for Chemically Fixed Tissue

      • Geoffrey R. Newman, Jan A. Hobot
      Pages 47-65

    5. Cryotechniques

      • Geoffrey R. Newman, Jan A. Hobot
      Pages 67-84

    6. Methods for Resin Polymerisation

      • Geoffrey R. Newman, Jan A. Hobot
      Pages 85-99

    7. Handling Resin Blocks

      • Geoffrey R. Newman, Jan A. Hobot
      Pages 101-105

  3. On-Section Immunolabelling

    1. Front Matter

      Pages 107-107
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    2. Strategies in Immunolabelling

      • Geoffrey R. Newman, Jan A. Hobot
      Pages 109-129

    3. General Considerations

      • Geoffrey R. Newman, Jan A. Hobot
      Pages 131-157

    4. Immunolabelling Protocols for Resin Sections

      • Geoffrey R. Newman, Jan A. Hobot
      Pages 159-175

    5. Resin Embedding and Immunolabelling

      • Geoffrey R. Newman, Jan A. Hobot
      Pages 177-181

  4. Back Matter

    Pages 183-221
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Keywords

About this book

For the first time, fixation, resin embedding and immunocytochemistry, the subjects of innumerable publications, have been organised into a comprehensive and coherent scheme showing how the various methodologies interrelate. Commercially available resins and their mode of use are described, and the book considers the advantages for cytochemistry and immunocytochemistry of matching tissue fixation to processing and resin embedding. On-section single and double immunolabelling methods are covered, using immunocolloidal gold and immunoperoxidase.

Authors and Affiliations

  • Electron Microscopy Unit, University of Wales College of Medicine, Cardiff, Great Britain

    Geoffrey R. Newman, Jan A. Hobot

Bibliographic Information

  • Book Title: Resin Microscopy and On-Section Immunocytochemistry

  • Authors: Geoffrey R. Newman, Jan A. Hobot

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-97481-6

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1993

  • eBook ISBN: 978-3-642-97481-6Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XIX, 221

  • Topics: Cell Biology, Immunology, Biochemistry, general, Anatomy

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