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Free Radicals and Antioxidant Protocols

  • Book
  • © 2010

Overview

Editors:
  1. Rao M. Uppu

    1. A& M College, Southern University &, Baton Rouge, U.S.A.

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  2. Subramanyam N. Murthy

    1. School of Medicine, Tulane University, New Orleans, U.S.A.

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  3. William A. Pryor

    1. Biodynamics Inst., Louisiana State University, Baton Rouge, U.S.A.

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  4. Narasimham L. Parinandi

    1. Dorothy M. Davis Heart &, Ohio State University, Columbus, U.S.A.

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  • Comprised of twenty-seven chapters contributed by world-renowned experts in the field
  • Fully updated from the first edition to offer cutting-edge methods for the rapid detection of RONs and redox stress
  • Includes in-depth analysis of natural and synthetic antioxidants, and also of DNA oxidation, oxidative lipidomics, and biomarkers
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 610)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Reactive Oxygen and Nitrogen Species

    1. Front Matter

      Pages 1-1
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    2. In Vivo Imaging of Free Radicals and Oxygen

      • Deepti S. Vikram, Brian K. Rivera, Periannan Kuppusamy
      Pages 3-27

    3. In Vivo Measurement of Tissue Oxygen Using Electron Paramagnetic Resonance Spectroscopy with Oxygen-Sensitive Paramagnetic Particle, Lithium Phthalocyanine

      • F. Hyodo, S. Matsumoto, E. Hyodo, A. Matsumoto, K. Matsumoto, M.C. Krishna
      Pages 29-39

    4. Measurement of Plasma Nitrite by Chemiluminescence

      • Enika Nagababu, Joseph M. Rifkind
      Pages 41-49

    5. Determination of Glutathione, Mitochondrial Transmembrane Potential, and Cytotoxicity in H9c2 Cardiomyoblasts Exposed to Reactive Oxygen and Nitrogen Species

      • K. Sathishkumar, Xueli Gao, Achuthan C. Raghavamenon, Subramanyam N. Murthy, Philip J. Kadowitz, Rao M. Uppu
      Pages 51-61

  3. Natural and Synthetic Antioxidants

    1. Front Matter

      Pages 63-63
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    2. Phenolic Acids and Flavonoids: Occurrence and Analytical Methods

      • Constantine D. Stalikas
      Pages 65-90

    3. Design, Synthesis, and Action of Antiatherogenic Antioxidants

      • Osamu Cynshi, Kunio Tamura, Etsuo Niki
      Pages 91-107

    4. Preparation of Drug-Loaded Polymeric Nanoparticles and Evaluation of the Antioxidant Activity Against Lipid Peroxidation

      • Adriana R. Pohlmann, Scheila Rezende Schaffazick, Tânia B. Creczynski-Pasa, Sílvia S. Guterres
      Pages 109-121

    5. Nanoparticle and Iron Chelators as a Potential Novel Alzheimer Therapy

      • Gang Liu, Ping Men, George Perry, Mark A. Smith
      Pages 123-144

    6. Synthesis and Characterization of Polymer Nanocarriers for the Targeted Delivery of Therapeutic Enzymes

      • Eric Simone, Thomas Dziubla, Vladimir Shuvaev, Vladimir R. Muzykantov
      Pages 145-164

    7. Assessment of Antioxidant Activity of Eugenol In Vitro and In Vivo

      • Enika Nagababu, Joseph M. Rifkind, Sesikeran Boindala, Lakshmaiah Nakka
      Pages 165-180

  4. Cellular Oxidative Stress

    1. Front Matter

      Pages 181-181
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    2. The Generation of Stable Oxidative Stress-Resistant Phenotypes in Chinese Hamster Fibroblasts Chronically Exposed to Hydrogen Peroxide or Hyperoxia

      • Douglas R. Spitz, Shannon J. Sullivan
      Pages 183-199

    3. A Simple Method for Effective and Safe Removal of Membrane Cholesterol from Lipid Rafts in Vascular Endothelial Cells: Implications in Oxidant-Mediated Lipid Signaling

      • Michelle A. Kline, E. S. O’Connor Butler, Adam Hinzey, Sean Sliman, Sainath R. Kotha, Clay B. Marsh et al.
      Pages 201-211

    4. Superoxide Dismutase – A Target for Gene Therapeutic Approach to Reduce Oxidative Stress in Erectile Dysfunction

      • W. Deng, T.J. Bivalacqua, H.C. Champion, W.J. Hellstrom, Subramanyam N. Murthy, Philip J. Kadowitz
      Pages 213-227

    5. Assessing the Reductive Capacity of Cells by Measuring the Recycling of Ascorbic and Lipoic Acids

      • James M. May
      Pages 229-243

    6. Biomarkers of Oxidative Stress: Methods and Measures of Oxidative DNA Damage (COMET Assay) and Telomere Shortening

      • Muthuswamy Balasubramanyam, Antonysunil Adaikalakoteswari, Zaheer Sameermahmood, Viswanathan Mohan
      Pages 245-261

    7. Simultaneous Analysis of Expression of Multiple Redox-Sensitive and Apoptotic Genes in Hypothalamic Neurons Exposed to Cholesterol Secoaldehyde

      • K. Sathishkumar, Achuthan C. Raghavamenon, Karunakaran Ganeshkumar, Rameshwari Telaprolu, Narasimham L. Parinandi, Rao M. Uppu
      Pages 263-284
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Keywords

About this book

In recent years there has been a growing demand to achieve more rapid and accurate detection of RONs (reactive oxygen and nitrogen species), which affect normal physiological processes and pathological conditions, and also of redox stress. In Free Radicals and Antioxidant Protocols, Second Edition, world-renowned researchers respond to this need, considering the most recent analytical and technological aspects of the detection and determination of RONs, and of oxidative, nitrative, nitrosative, and redox stress in biological systems in vivo and in vitro. Chapters include protocols to establish biomarkers for the oxidative stress-induced genetic damage in cellular and animal models, provide novel methods and technologies for analyzing peroxidized lipids, describe techniques for gene therapy, and present methods of EPR imaging of free radicals and oxygen in vivo. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and user-friendly, Free Radicals and Antioxidant Protocols, Second Edition, is an essential guide for the novice and expert researcher alike.

Reviews

From the reviews of the second edition:

“The book is organized into four sections which draw on contributions from several experts in the field of oxidative stress. … Based on a collection of articles in the four sections, the book is a good reference for those interested in free radical science including new methodologies to measure both oxidative stress and antioxidants using in vivo and in vitro models.” (Silvia Berlanga de Moraes Barros, Brazilian Journal of Pharmaceutical Sciences, May, 2010)

Editors and Affiliations

  • A& M College, Southern University &, Baton Rouge, U.S.A.

    Rao M. Uppu

  • School of Medicine, Tulane University, New Orleans, U.S.A.

    Subramanyam N. Murthy

  • Biodynamics Inst., Louisiana State University, Baton Rouge, U.S.A.

    William A. Pryor

  • Dorothy M. Davis Heart &, Ohio State University, Columbus, U.S.A.

    Narasimham L. Parinandi

Bibliographic Information

  • Book Title: Free Radicals and Antioxidant Protocols

  • Editors: Rao M. Uppu, Subramanyam N. Murthy, William A. Pryor, Narasimham L. Parinandi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60327-029-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2010

  • Hardcover ISBN: 978-1-58829-710-5Published: 23 December 2009

  • Softcover ISBN: 978-1-61779-683-8Published: 06 April 2012

  • eBook ISBN: 978-1-60327-029-8Published: 15 December 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVI, 480

  • Number of Illustrations: 104 b/w illustrations, 11 illustrations in colour

  • Additional Information: Originally published as volume 108 in the series: Methods Molecular Biology

  • Topics: Biochemistry, general, Analytical Chemistry, Oxidative Stress

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