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Comparative proteomic analysis of Salmonella typhimurium LT2 and its hisG gene inactivated mutant

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Abstract

A hisG gene, encoding ATP phosphoribosyl phosphothansferase, insertion mutation was found to effected the production of four different proteins in Salmonella Typhimurium LT2 as a results of comparative analysis of two-dimensional analysis of hisG mutant (Δhis87) and the conrol strain (LT2). MALDI-TOF analysis of these protein spots which were expresed in S. Typhimurium LT2 but not its mutant Δhis87, showed that these proteins were; GMP synthase, arginine-tRNA ligase (Arginyl-tRNA synthetase), SopE and SifA. All of these proteins are virulence-associated proteins in Salmonella. Thus we have concluded that the proper function of histidine operon is very crucial for virulence of S. Typhimurium.

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Authors and Affiliations

  1. Faculty of Science and Literature, Biology Department, Ahi Evran University, Bagbasi, Kirsehir, 40100, Turkey

    I. Erdogan

  2. Institute of Biotechnology, Ankara Univeristy, Tandogan, Ankara, 06100, Turkey

    N. Akcelik

  3. Faculty of Science, Biology Department, Ankara University, Tandogan, Ankara, 06100, Turkey

    I. Erdogan & M. Akcelik

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  3. M. Akcelik
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Correspondence to I. Erdogan.

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Erdogan, I., Akcelik, N. & Akcelik, M. Comparative proteomic analysis of Salmonella typhimurium LT2 and its hisG gene inactivated mutant. Mol. Genet. Microbiol. Virol. 30, 48–56 (2015). https://doi.org/10.3103/S0891416815010024

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