Abstract
Painful diabetic neuropathy (PDN) is a diabetes mellitus complication. Unfortunately, the mechanisms underlying PDN are still poorly understood. Adenosine triphosphate (ATP)-gated P2X7 receptor (P2X7R) plays a pivotal role in non-diabetic neuropathic pain, but little is known about its effects on streptozotocin (STZ)-induced peripheral neuropathy. Here, we explored whether spinal cord P2X7R was correlated with the generation of mechanical allodynia (MA) in STZ-induced type 1 diabetic neuropathy in mice. MA was assessed by measuring paw withdrawal thresholds and western blotting. Immunohistochemistry was applied to analyze the protein expression levels and localization of P2X7R. STZ-induced mice expressed increased P2X7R in the dorsal horn of the lumbar spinal cord during MA. Mice injected intrathecally with a selective antagonist of P2X7R and P2X7R knockout (KO) mice both presented attenuated progression of MA. Double-immunofluorescent labeling demonstrated that P2X7R-positive cells were mostly co-expressed with Iba1 (a microglia marker). Our results suggest that P2X7R plays an important role in the development of MA and could be used as a cellular target for treating PDN.
摘要
目 的
探讨嘌呤能离子通道型受体 7 (P2X7R) 在链脲佐菌素 (STZ) 诱导的 1 型糖尿病 (T1DM) 小鼠早期发生的机械痛性神经病变中的作用。
创新点
探讨脊髓 P2X7R 在 T1DM 产生痛性神经病变的早期阶段所起的作用, 将有望为研究糖尿病痛性神经病变药物提供新的靶点。
方 法
本实验采用健康雄性 C57BL 小鼠与 P2X7R KO 小鼠(体重 20∼30 克, 从 20 点至次日 8 点隔夜禁食 12 小时)为研究对象, 连续三天腹腔注射 STZ (浓度为 100 mg/kg), 从而制备 T1DM 动物模 型。如果空腹血糖 >11.1 mmol/L 且三周后小鼠机 械痛阈值明显下降, 则表示模型制备成功。在小鼠机械痛阈下降的对应时间点, 取腰段脊髓背角, 采用蛋白质印迹法 (western blot) 和免疫组化方法测定 P2X7R 的表达情况。同时, 在发生机械痛阈值下降的第 3 周时间鞘内给予其拮抗剂 A740003, 并进行机械刺激从而观察其痛阈值的变化。
结 论
STZ 诱导的 T1DM 动物模型在早期表现为显著的机械诱发痛, 并伴随脊髓背角 P2X7R 表达上调; 在痛阈下降期鞘内给予其拮抗剂 A740003 可抑制糖尿病小鼠的痛行为。 与腹腔注射 STZ 形成 T1DM 的 C57BL/6 小鼠相比, P2X7R 基因敲除的糖尿病小鼠早期机械痛阈值下降的时间延迟, 程度减轻。因此, 我们推测 P2X7R 可能参与了 STZ 诱导的糖尿病小鼠早期机械痛性神经病变。
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Cheng-ming NI was responsible for drafting the manuscript, analysis and interpretation of data. He-ping SUN collected and analyzed the data. Xiang XU, Bing-yu LING, and Hui JIN contributed analysis of data and manuscript preparation. Yu-qiu ZHANG and Zhi-qi ZHAO helped perform the analysis with constructive discussions. Hong CAO and Lan XU contributed the conception and design of the current study. All authors have read and approved the final manuscript. Therefore, all authors had full access to all the data in the study and take responsibility for the integrity and security of the data.
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Cheng-ming NI, He-ping SUN, Xiang XU, Bing-yu LING, Hui JIN, Yu-qiu ZHANG, Zhi-qi ZHAO, Hong CAO, and Lan XU declare that they have no conflict of interest.
All institutional and national guidelines for the care and use of laboratory animals were followed.
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Project supported by the National Natural Science Foundation of China (Nos. 81771208 and 81971043), the Health and Family Planning Commission of Wuxi (No. YGZXM1406), the Wuxi Municipal Bureau on Science and Technology (No. CSE31N1614), the Fundamental Research Fund of Wuxi People’s Hospital (No. RKA201720), and the Technology for Social Development Project of Kunshan (No. KS1539), China
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Ni, Cm., Sun, Hp., Xu, X. et al. Spinal P2X7R contributes to streptozotocin-induced mechanical allodynia in mice. J. Zhejiang Univ. Sci. B 21, 155–165 (2020). https://doi.org/10.1631/jzus.B1900456
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DOI: https://doi.org/10.1631/jzus.B1900456