Abstract
Royal jelly (RJ) from honeybee has been widely used as a health promotion supplement. The major royal jelly proteins (MRJPs) have been identified as the functional component of RJ. However, the question of whether MRJPs have anti-senescence activity for human cells remains. Human embryonic lung fibroblast (HFL-I) cells were cultured in media containing no MRJPs (A), MRJPs at 0.1 mg/ml (B), 0.2 mg/ml (C), or 0.3 mg/ml (D), or bovine serum albumin (BSA) at 0.2 mg/ml (E). The mean population doubling levels of cells in media B, C, D, and E were increased by 12.4%, 31.2%, 24.0%, and 10.4%, respectively, compared with that in medium A. The cells in medium C also exhibited the highest relative proliferation activity, the lowest senescence, and the longest telomeres. Moreover, MRJPs up-regulated the expression of superoxide dismutase-1 (SOD1) and down-regulated the expression of mammalian target of rapamycin (MTOR), catenin beta like-1 (CTNNB1), and tumor protein p53 (TP53). Raman spectra analysis showed that there were two unique bands related to DNA synthesis materials, amide carbonyl group vibrations and aromatic hydrogens. These results suggest that MRJPs possess anti-senescence activity for the HFL-I cell line, and provide new knowledge illustrating the molecular mechanism of MRJPs as anti-senescence factors.
摘要
目的
探讨不同浓度蜂王浆主蛋白(MRJPs)对人胚肺成纤维细胞HFL-I的抗衰老作用,并对其可能的分子机理进行预测。
创新点
本实验采用拉曼光谱法探究添加MRJPs对HFL-I细胞内成分的影响。实验发现添加MRJPs的HFL-I与对照组的HFL-I的拉曼光谱图中,与DNA和蛋白质合成相关的拉曼峰发生了变化。该发现 在未来可作为鉴定衰老HFL-I的一种手段。
方法
实验以蜂王浆为原料,利用透析法得到MRJPs。通过对不同浓度MRJPs处理的HFL-I进行细胞形态学观察,测定HFL-I生命周期以及检测其β-半乳糖苷酶活性,评价了MRJPs的抗衰老效果。并且 通过MTT法考察了不同浓度MRJPs对HFL-I的促 增殖作用。同时,实验一方面采用实时定量聚合酶链反应(qPCR)测量了不同浓度MRJPs处理的 HFL-I的细胞端粒相对长度以及MTOR、SOD1、CTNNB1、TP53四种衰老相关基因的相对表达量; 另一方面还通过拉曼光谱实验法,进一步探索了MRJPs抗衰老的分子机理。
结论
本实验结果显示,添加MRJPs能降低HFL-I衰老细胞所占比例,显著延长HFL-I的生命周期,一定量的MRJPs还能够促进HFL-I的增殖。此外,MRJPs还能减缓细胞端粒长度的缩短,上调衰老 相关基因SOD1的表达,下调抗衰老基因MTOR、CTNNB1和TP53的表达。通过MRJPs添加组细胞的拉曼光谱上出现的独特的碱基、酰胺羰基和芳香氢峰,推测MRJPs的抗衰老作用可能与促进 DNA及蛋白质在细胞中的合成密切相关。
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Project supported by the Science and Technology Project of Zhejiang Province (No. 2017C32033) and the National Natural Science Foundation of China (No. 3127848)
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Jiang, Cm., Liu, X., Li, Cx. et al. Anti-senescence effect and molecular mechanism of the major royal jelly proteins on human embryonic lung fibroblast (HFL-I) cell line. J. Zhejiang Univ. Sci. B 19, 960–972 (2018). https://doi.org/10.1631/jzus.B1800257
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DOI: https://doi.org/10.1631/jzus.B1800257
Key words
- Major royal jelly protein
- Human embryonic lung fibroblast (HFL-I) cell line
- Anti-senescence
- Relative proliferation activity
- Telomere length
- Molecular mechanism