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Chemical composition and antioxidant activity of certain Morus species

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Abstract

In the present work, the fruits of four Morus species, namely Morus alba (white mulberry), Morus nigra (black mulberry), Morus laevigata (large white fruit), and Morus laevigata (large black fruit), were analyzed for proximate composition, essential minerals, and antioxidant potentials. For this purpose, the ripe fruits were collected from the northern regions of Pakistan. The major nutritional components (moisture, ash, lipids, proteins, fibres, carbohydrates, and total sugar) were found to be in the suitable range along with good computed energy. Total dry weight, pH, and titratable acidity (percent citric acid) were (17.60±1.94)–(21.97±2.34) mg/100 g, (3.20±0.07)–(4.78±0.15), and (0.84±0.40)%–(2.00±0.08)%, respectively. Low riboflavin (vitamin B2) and niacin (vitamin B3) contents were recorded in all the fruits, while ascorbic acid (vitamin C) was in the range from (15.20±1.25) to (17.03±1.71) mg/100 g fresh weight (FW). The mulberry fruits were rich with regard to the total phenol and alkaloid contents, having values of (880±7.20)–(1650±12.25) mg/100 g FW and (390±3.22)–(660±5.25) mg/100 g FW, respectively. Sufficient quantities of essential macro-(K, Ca, Mg, and Na) and micro-(Fe, Zn, and Ni) elements were found in all the fruits. K was the predominant element with concentration ranging from (1270±9.36) to (1731±11.50) mg/100 g, while Ca, Na, and Mg contents were (440±3.21)–(576±7.37), (260±3.86)–(280±3.50), and (240±3.51)–(360±4.20) mg/100 g, respectivly. The decreasing order of micro-minerals was Fe>Zn>Ni. The radical scavenging activity of methanolic extract of fruits was concentration-dependent and showed a correlation with total phenolic constituents of the respective fruits. Based on the results obtained, mulberry fruits were found to serve as a potential source of food diet and natural antioxidants.

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References

  • Al-Bayati, F.A., Al-Mola, H.F., 2008. Antibacterial and antifungal activities of different parts of Tribulus terrestris L. growing in Iraq. J. Zhejiang Univ.-Sci. B, 9(2):154–159. [doi: 10.1631/jzus.B0720251]

    Article  PubMed  Google Scholar 

  • AOAC (Association of Official Analytical Chemists), 2000. Official Methods of Analysis International, 17th Ed. AOAC, Washington, DC.

    Google Scholar 

  • Arabshahi-Delouee, S., Urooj, A., 2007. Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves. Food Chem., 102(4):1233–1240. [doi:10.1016/j.foodchem.2006.07.013]

    Article  CAS  Google Scholar 

  • Christie, W.W., 1983. Lipids. In: Aliphatic and Related Natural Product Chemistry, 2nd Ed. Pergamon Press, Oxford.

    Google Scholar 

  • Cieslik, E., Greda, A., Adamus, W., 2006. Contents of polyphenols in fruit and vegetables. Food Chem., 94(1): 135–142. [doi:10.1016/j.foodchem.2004.11.015]

    Article  CAS  Google Scholar 

  • Darias-Martin, J., Lobo-Rodrigo, G., Hernandez-Cordero, J., Diaz-Diaz, E., Diaz-Romero, C., 2003. Alcoholic beverages obtained from black mulberry. Food Technol. Biotechnol., 41(2):173–176.

    CAS  Google Scholar 

  • Duke, J.A., Ayensu, E.S., 1985. Medicinal Plants of China. Reference Publications Inc., Algonac, MI.

    Google Scholar 

  • Elmacı, Y., Altuğ, T., 2002. Flavour evaluation of three black mulberry (Morus nigra) cultivars using GC/MS, chemical and sensory data. J. Sci. Food Agric., 82(6):632–635. [doi:10.1002/jsfa.1085]

    Article  CAS  Google Scholar 

  • Ercisli, S., Orhan, E., 2007. Chemical composition of white (Morus alba), red (Morus rubra) and black (M. nigra) mulberry fruits. Food Chem., 103(4):1380–1384. [doi:10.1016/j.foodchem.2006.10.054]

    Article  CAS  Google Scholar 

  • Fogliano, V., Verde, V., Randazzo, G., Ritieni, A., 1999. Method for measuring antioxidant activity and its application to monitoring the antioxidant capacity of wines. J. Agric. Food Chem., 47(3):1035–1040. [doi:10.1021/jf980496s]

    Article  CAS  PubMed  Google Scholar 

  • Gao, X., Ohlander, M., Jeppsson, N., Björk, L., Trajkovski, V., 2000. Changes in antioxidant effects and their relationship to phytonutrients in fruits of sea buckthorn (Hippophae rhamnoides L.) during maturation. J. Agric. Food Chem., 48(5):1485–1490. [doi:10.1021/jf991072g]

    Article  CAS  PubMed  Google Scholar 

  • Gerasopoulos, D., Stavroulakis, G., 1997. Quality characteristics of four mulberry (Morus sp.) cultivars in the area of Chania, Greece. J. Sci. Food Agric., 73(2):261–264. [doi: 10.1002/(SICI)1097-0010(199702)73:2<261::AID-JSFA724>3.0.CO;2-S]

    Article  CAS  Google Scholar 

  • Hou, Y.J., 1994. Mulberry Breeding. PhD Thesis, Sericulture Department, Zhejiang Agriculture University, Hangzhou, China (in Chinese).

    Google Scholar 

  • Humphry, C.M., Clegg, M.S., Keen, C.L., Grivetti, L.E., 1993. Food diversity and drought survival. The Hausa example. Int. J. Food Sci. Nutr., 44(1):1–16. [doi:10.3109/09637489309017417]

    Article  CAS  Google Scholar 

  • Hussain, I., Khan, H., 2010. Investigation of heavy metals content in medicinal plant, Eclipta alba L. J. Chem. Soc. Pak., 32(1):28–33.

    CAS  Google Scholar 

  • Hussain, I., Khan, L., Mehmood, T., Khan, I., Ullah, W., Khan, H., 2005. Effect of heavy metals on the growth and development of Silybum marianum, in various polluted areas of Peshawar, Pakistan. J. Chem. Soc. Pak., 27(4): 367–373.

    CAS  Google Scholar 

  • Hussain, I., Khan, L., Marwat, G.A., Ahmed, N., Saleem, M., 2008. Comparative study of vitamin C contents in fruits and medicinal plants. J. Chem. Soc. Pak., 30(3):406–409.

    CAS  Google Scholar 

  • Ikhtiar, K., Alam, Z., 2007. Nutritional composition of Pakistani wheat varieties. J. Zhejiang Univ.-Sci. B, 8(8):555–559. [doi: 10.1631/jzus.2007.B0555]

    Article  CAS  PubMed  Google Scholar 

  • Imran, M., Talpur, F.N., Jan, M.S., Khan, A., Khan, I., 2007. Analysis of nutritional components of some wild edible plants. J. Chem. Soc. Pak., 29(5):500–508.

    CAS  Google Scholar 

  • Imran, M., Khan, H., Hassan, S.S., Khan, R., 2008. Physicochemical characteristics of various milk samples available in Pakistan. J. Zhejiang Univ.-Sci. B, 9(7):546–551. [doi: 10.1631/jzus.B0820052]

    Article  CAS  PubMed  Google Scholar 

  • Khan, T., Ahmad, M., Khan, R., Khan, H., Ejaz, A., Choudhary, M.I., 2006. Evaluation of phytomedicinal potentials of selected plants of Pakistan. Am. Lab., 38(9):20–22.

    CAS  Google Scholar 

  • Kjeldahl, J., 1983. Determination of protein nitrogen in food products. Encyc. Food Agric., 28:757–765.

    Google Scholar 

  • Lin, J.Y., Tang, C.Y., 2007. Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food Chem., 101(1):140–147. [doi:10.1016/j.foodchem.2006.01.014]

    Article  CAS  Google Scholar 

  • Muler, H.G., Tobin, G., 1980. Nutrition of Food Processing. Croom Helm Ltd., London.

    Google Scholar 

  • Okwu, D.E., 2005. Phytochemicals, vitamins and mineral contents of two Nigerian medicinal plants. Int. J. Mol. Med. Adv. Sci., 1(4):375–381.

    Google Scholar 

  • Onyeike, E.N., Ikru, P.R., 1998. Preliminary investigation of proximate composition of heat processed cashew nut seed (Anacardium occidentale) flours. J. Appl. Sci. Environ. Manage., 1(1):27–30.

    Google Scholar 

  • Peterson, D.M., Emmons, C.L.L., Hibbs, A.H., 2001. Phenolic antioxidants and antioxidant activity in pearling fractions of oat groats. J. Cereal Sci., 33(1):97–103. [doi:10.1006/jcrs.2000.0347]

    Article  CAS  Google Scholar 

  • Sass-Kiss, A., 2005. Differences in anthocyanin and carotenoid content of fruits and vegetables. Food Res. Int., 38(8–9):1023–1029. [doi:10.1016/j.foodres.2005.03.014]

    Article  CAS  Google Scholar 

  • Zadernowski, R., Naczk, M., Nesterowicz, J., 2005. Phenolic acid profiles in some small berries. J. Agric. Food Chem., 53(6):2118–2124. [doi:10.1021/jf040411p]

    Article  CAS  PubMed  Google Scholar 

  • Zheng, W., Wang, S.Y., 2001. Antioxidant activity and phenolic compounds in selected herbs. J. Agric. Food Chem., 49(11):5165–5170. [doi:10.1021/jf010697n]

    Article  CAS  PubMed  Google Scholar 

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Imran, M., Khan, H., Shah, M. et al. Chemical composition and antioxidant activity of certain Morus species. J. Zhejiang Univ. Sci. B 11, 973–980 (2010). https://doi.org/10.1631/jzus.B1000173

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  • DOI: https://doi.org/10.1631/jzus.B1000173

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