Abstract
Ultrafiltration and a series of chromatographic steps were used to isolate and purify polysaccharides from Tremella aurantialba fruit bodies. Three crude fractions (TAP50w, TAP10-50w, and TAP1-10w), five semi-purified fractions (TAPA-TAPE), and one purified fraction (TAPA1) were obtained. A sulfated derivative of TAPA1 (TAPA1-s) was prepared by chemical modification. The immunostimulating activity of the polysaccharide fractions in vitro was determined using the mouse spleen lymphocyte proliferation assay. Of the three crude fractions tested, cell proliferation rates were increased most by TAP50w. Furthermore, TAPA1-s was markedly more stimulatory than TAPA1, indicating that sulfonation was an effective way to enhance the immunostimulating activity of polysaccharide.
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References
Bohn, J.A., BeMiller, J.N., 1995. (1→3)-β-D-glucans as biological response modifiers: a review of structure-functional activity relationships. Carbohydrate Polymers, 28(1):3–14. [doi:10.1016/0144-8617(95)00076-3]
Chaidedgumjorn, A., Toyoda, H., Woo, E.R., Lee, K.B., Kim, Y.S., Toida, T., Imanari, T., 2002. Effect of (1→3)- and (1→4)-linkages of fully sulfated polysaccharides on their anticoagulant activity. Carbohydrate Research, 337(10): 925–933. [doi:10.1016/S0008-6215(02)00078-2]
Cui, H.X., Liu, Q., Tao, Y.Z., Zhang, H.F., Zhang, L.N., Ding, K., 2008. Structure and chain conformation of a (1→6)-α-D-glucan from the root of Pueraria lobata (Willd.) Ohwi and the antioxidant activity of its sulfated derivative. Carbohydrate Polymers, 74(4):771–778. [doi: 10.1016/j.carbpol.2008.04.034]
de Baets, S., Vandamme, E.J., 2001. Extracellular Tremella polysaccharides: structure, properties and applications. Biotechnology Letters, 23(17):1361–1366. [doi:10.1023/A:1011645724220]
de Baets, S., du Laing, S., François, C., Vandamme, E.J., 2002. Optimization of exopolysaccharide production by Tremella mesenterica NRRL Y-6158 through implementation of fed-batch fermentation. Journal of Industrial Microbiology and Biotechnology, 29(4):181–184. [doi:10.1038/sj.jim.7000276]
Du, X.J., Zhang, J.S., Yang, Y., Ye, L.B., Tang, Q.J., Jia, W., Liu, Y.F., Zhou, S., Hao, R.X., Gong, C.Y., et al., 2009. Structural elucidation and immuno-stimulating property of an acidic heteropolysaccharide (TAPA1) from Tremella aurantialba. Carbohydrate Research, 344(5): 672–678. [doi:10.1016/j.carres.2009.01.021]
Duarte, M.E.R., Cardoso, M.A., Noseda, M.D., Cerezo, A.S., 2001. Structural studies on fucoidans from the brown seaweed Sargassum stenophyllum. Carbohydrate Research, 333(4):281–293. [doi:10.1016/S0008-6215(01)00 149-5]
Dubois, M., Gillis, K.A., Hamilton, J.K., Rebers, P.A., Smith, F., 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28(3): 350–356. [doi:10.1021/ac60111a017]
Han, F., Yao, W., Yang, X., Liu, X., Gao, X., 2005. Experimental study on anticoagulant and antiplatelet aggregation activity of a chemically sulfated marine polysaccharide YCP. International Journal of Biological Macromolecules, 36(4):201–207. [doi:10.1016/j.ijbiomac.2005.06.003]
Inoue, K., Kawnmoto, K., Nakajimo, H., Kohno, M., Kadoya, S., Mizunoet, D., 1983. Chemical modification and antitumor activity of a D-manno-D-glucan from Microellobosporia grisea. Carbohydrate Research, 115:199–208. [doi:10.1016/0008-6215(83)88148-8]
Kiho, T., Kochi, M., Usui, S., Hirano, K., Aizawa, K., Inakuma, T., 2001. Antidiabetic effect of an acidic polysaccharide (TAP) from Tremella aurantia and its degradation product (TAP-H). Biological & Pharmaceutical Bulletin, 24(12):1400–1403. [doi:10.1248/bpb.24.1400]
Ma, L., Lin, Z., 1992. Effect of Tremella polysaccharide on IL-2 production by mouse splenocytes. Acta Pharmaceutica Sinica, 27:1–4 (in Chinese).
Nie, X., Shi, B., Ding, Y., Tao, W., 2006. Preparation of a chemically sulfated polysaccharide derived from Grifola frondosa and its potential biological activities. International Journal of Biological Macromolecules, 39(4–5): 228–233. [doi:10.1016/j.ijbiomac.2006.03.030]
Peng, Y., Zhang, L., Zeng, F., Kennedy, J.F., 2005. Structure and antitumor activities of the water-soluble polysaccharides from Ganoderma tsugae mycelium. Carbohydrate Polymers, 59(3):385–392. [doi:10.1016/j.carbpol.2004.10.009]
Shen, X.X., Yang, Y., Zhang, J.S., Tang, Q.J., Jia, W., 2008. Isolation, purification and immunomodifying activity of polysaccharide from Phellinus igniarius. Acta Edulis Fungi, 15(2):37–41 (in Chinese).
Su, Z., Dai, Z., Yang, J., 2006. Research progress on immunologic mechanism of polysaccharide. Journal of Yunnan Agricultural University, 21(2):205–209 (in Chinese).
Ukai, S., Hirose, K., Kiho, T., Hara, C., Irikura, T., 1972. Antitumor activity on sarcoma 180 of the polysaccharides from Tremella fuciformis Berk. Chemical & Pharmaceutical Bulletin, 20:2293–2294.
Wang, H., Qu, W., Chu, S., Li, M., Tian, C., 2002. Studies on the preventive and therapeutic effects of the polysaccharide of Tremella aurantialba mycelia on diet-induced hyperlipidemia in mice. Acta Nutrimenta Sinica, 24(4): 431–432 (in Chinese).
Wang, L., Huang, H.Y., Wei, Y.Y., Li, X.X., Chen, Z.X., 2009. Characterization and anti-tumor activities of sulfated polysaccharide SRBPS2a obtained from defatted rice bran. International Journal of Biological Macromolecules, 45(4):427–431. [doi:10.1016/j.ijbiomac.2009.06.006]
Zhang, J.S., Tang, Q.J., Zimmerman-Kordmann, M., Reutter, W., Fan, H., 2002. Activation of B lymphocytes by GLIS, a bioactive proteoglycan from Ganoderma lucidum. Life Sciences, 71(6):623–638. [doi:10.1016/S0024-3205(02)01690-9]
Zhang, L., Chen, L., Xu, X., Lin, Y., Cheung, P.C.K., Kennedy, J.F., 2005. Comparison on chain stiffness of a water-insoluble (1→3)-α-D-glucan isolated from Poria cocos mycelia and its sulfated derivative. Carbohydrate Polymers, 59(2):257–263. [doi:10.1016/j.carbpol.2004.09.017]
Zhang, W., Qu, W., Zhang, X., 2004. The anti-hyperglycemic activity of polysaccharides from Tremella aurantialba mycelium. Acta Nutrimenta Sinica, 26(4):300–303 (in Chinese).
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Project (No. 2006BAD06B08) supported by the National Key Technology R & D Program of China
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Du, Xj., Zhang, Js., Yang, Y. et al. Purification, chemical modification and immunostimulating activity of polysaccharides from Tremella aurantialba fruit bodies. J. Zhejiang Univ. Sci. B 11, 437–442 (2010). https://doi.org/10.1631/jzus.B0900402
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DOI: https://doi.org/10.1631/jzus.B0900402