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Evaluation of three-dimensional silver-doped borate bioactive glass scaffolds for bone repair: Biodegradability, biocompatibility, and antibacterial activity

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Abstract

The development of synthetic scaffolds with a desirable combination of properties, such as bioactivity, the ability to locally deliver antibacterial agents and high osteogenic capacity, is a challenging but promising approach in bone tissue engineering. In this study, scaffolds of a borosilicate bioactive glass (composition: 6Na2O, 8K2O, 8MgO, 22CaO, 36B2O3, 18SiO2, 2P2O5; mol%) with controllable antibacterial activity were developed by doping the parent glass with varying amounts of Ag2O (0.05, 0.5, and 1.0 wt%). The addition of the Ag2O lowered the compressive strength and degradation of the bioactive glass scaffolds but it did not affect the formation of hydroxyapatite on the surface of the glass as determined by energy dispersive x-ray analysis, x-ray diffraction, and Fourier transform infrared analysis. The Ag2O-doped scaffolds showed a sustained release of Ag ions over more than 8 weeks in simulated body fluid and resistance against colonization by the bacterial strains Escherichia coli and Staphylococcus aureus. In vitro cell culture showed better adhesion, proliferation, and alkaline phosphatase activity of murine osteoblastic MC3T3-E1 cells on the Ag2O-doped bioactive glass scaffolds than on the undoped scaffolds. The results indicate that these Ag-doped borosilicate bioactive glass scaffolds may have potential in repairing bone coupled with providing a lower risk of bacterial infection.

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ACKNOWLEDGMENTS

This work was supported by the Science and Technology Commission of Shanghai Municipality (Grant No. 12JC1408500) and the National Natural Science Foundation, China (Grant Nos. 51072133, 81201377 and 51372170).

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Materials Science and Engineering, Institute of Bioengineering and Information Technology Materials, Tongji University, Shanghai, 200092, China

    Hui Wang

  2. Department of Orthopedic Surgery, Shanghai Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai, 200233, China

    Shichang Zhao & Changqing Zhang

  3. School of Materials Science and Engineering, Tongji University, Shanghai, 200092, China

    Xu Cui, Yangyi Pan, Wenhai Huang, Song Ye & Deping Wang

  4. Department of Orthopaedic Surgery, Shanghai Jiaotong University Affiliated Ruijing Hospital, Shanghai, 200233, China

    Shihua Luo

  5. Department of Materials Science and Engineering, and Center for Biomedical Science and Engineering, Missouri University of Science and Technology, Rolla, Missouri, 65409, USA

    Mohamed N. Rahaman

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  1. Hui Wang
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Correspondence to Changqing Zhang or Deping Wang.

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Wang, H., Zhao, S., Cui, X. et al. Evaluation of three-dimensional silver-doped borate bioactive glass scaffolds for bone repair: Biodegradability, biocompatibility, and antibacterial activity. Journal of Materials Research 30, 2722–2735 (2015). https://doi.org/10.1557/jmr.2015.243

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