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Stability-Indicating Densitometric HPTLC Method for Qualitative and Quantitative Analysis of Hydroquinone in Commercial Whitening Creams

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Summary

A densitometric high-performance thin-layer chromatographic (HPTLC) method for analysis of hydroquinone has been developed and validated. Chromatography was performed on aluminum foilbacked silica gel 60 F254 plates with chloroform-methanol 85:15 (% v/v) as mobile phase. This system furnished a compact band for hydroquinone at RF 0.51. Hydroquinone was quantified densitometrically at 289 nm. The limits of detection (LOD) and quantification (LOQ) were 38.50 and 115.50 ng per band, respectively. High precision and accuracy were achieved. The method was used for both qualitative and quantitative analysis of hydroquinone in commercial formulations. Because the method can effectively separate the hydroquinone in the presence of its degradation products, it can be used as a stability-indicating method.

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References

  1. M.C. Spencer, Arch. Dermatol. 84 (1961) 131–134.

    Article  Google Scholar 

  2. M.C. Spencer, S.W. Becker, Clin. Med. 70 (1963) 1111–1114.

    CAS  PubMed  Google Scholar 

  3. K. Jimbow, H. Obata, A. Pathak, T.B. Fitzpatrick, J. Invest. Dermatol. 62 (1974) 436–449.

    Article  CAS  Google Scholar 

  4. K.B. Penney, C.J. Smith, C.J. Allen, J. Invest. Dermatol. 82 (1984) 308–310.

    Article  CAS  Google Scholar 

  5. A. Palumbo, M. d’Ischia, G. Misuraca, G. Prota, Biochem. Biophys. Acta 1073 (1991) 85–90.

    Article  CAS  Google Scholar 

  6. A. Katsambas, C.H. Antoniou, J. Eur. Acad. Dermatol. Venereol. 4 (1995) 217–223.

    Article  Google Scholar 

  7. A. Mohamed, M. Mohamed, Int. J. Dermatol. 37 (1998) 449–450.

    Article  Google Scholar 

  8. J. Firth, I. Rix, Analyst 111 (1986) 129–132.

    Article  CAS  Google Scholar 

  9. L. Gagliardi, A. Amato, G. Cavazzutti, F. Chimenti, A. Bolasco, D. Tonelli, J. Chromatogr. 404 (1987) 267–272.

    Article  CAS  Google Scholar 

  10. M. Borremans, J. De-Beer, L. Goeyens, Chromatographia 50 (1999) 346–352.

    Article  CAS  Google Scholar 

  11. United States Pharmacopeia, 28th edn., United States Pharmacopeial Convention, Rockville, 2005, pp. 974–975.

  12. C. Desiderio, L. Ossicini, S. Fanali, J. Chromatogr. A 887 (2000) 489–496.

    Article  CAS  Google Scholar 

  13. I.K. Sakodinskaya, C. Desiderio, A. Nardi, S. Fanali, J. Chromatogr. 596 (1992) 95–100.

    Article  CAS  Google Scholar 

  14. L. Wang, Analyst 120 (1995) 2241–2244.

    Article  CAS  Google Scholar 

  15. V.I. Cruz, O. Fatibello-Filho, Talanta 52 (2000) 681–689.

    Article  Google Scholar 

  16. M.E. Rueda, L.A. Sarabia, A. Herrero, M.C. Ortiz, Anal. Chim. Acta 479 (2003) 173–184.

    Article  CAS  Google Scholar 

  17. J. Ali, N. Ali, Y. Sultana, S. Baboota, S. Faiyaz, Acta Chromatogr. 18 (2007) 168–179.

    CAS  Google Scholar 

  18. M.S. Faisal, Z. Naz, F. Shakeel, S. Ahmed, K. Kohli, R.K. Khar, Chem. Anal. 54 (2009) 77–88.

    CAS  Google Scholar 

  19. P. Alam, M. Ali, R. Singh, F. Shakeel, Acta Chromatogr. 21 (2009) 683–692.

    Article  CAS  Google Scholar 

  20. S. Ahmad, G.K. Jain, M. Faiyazuddin, Z. Iqbal, S. Talegaonkar, Y. Sultana, F.J. Ahmad, Acta Chromatogr. 21 (2009) 631–639.

    Article  CAS  Google Scholar 

  21. International Conference on Harmonisation (ICH) of Technical Requirements for Registration of Pharmaceuticals for Human Use: Harmonised Triplicate Guideline on Validation of Analytical Procedures: Methodology, Recommended for Adoption at Step 4 of the ICH Process on November 1996 by the ICH Steering Committee, IFPMA, Geneva.

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Authors and Affiliations

  1. Department of Pharmacognosy, College of Pharmacy, Al Kharj University, Al Kharj, Kingdom of Saudi Arabia

    Saleh I. Alqasoumi, Prawez Alam & Maged S. Abdel-Kader

  2. Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia

    Adnan J. Al-Rehaily

  3. Department of Pharmaceutics, Faculty of Pharmacy, Al-Arab Medical University, Benghazi, Libya

    Faiyaz Shakeel

Authors
  1. Saleh I. Alqasoumi
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  2. Prawez Alam
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  3. Adnan J. Al-Rehaily
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  4. Faiyaz Shakeel
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  5. Maged S. Abdel-Kader
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Correspondence to Prawez Alam.

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Alqasoumi, S.I., Alam, P., Al-Rehaily, A.J. et al. Stability-Indicating Densitometric HPTLC Method for Qualitative and Quantitative Analysis of Hydroquinone in Commercial Whitening Creams. JPC-J Planar Chromat 24, 48–52 (2011). https://doi.org/10.1556/JPC.24.2011.1.9

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  • DOI: https://doi.org/10.1556/JPC.24.2011.1.9

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