Abstract
Modification of Xanthophyllomyces dendrorhous toward a higher carotenoid synthesis will make the exploitation of X. dendrorhous as a natural source for astaxanthin more competitive. By recombinant DNA technology, the X. dendrorhous isopentenyl-pyrophosphate isomerase-encoding gene (idi) was inserted in a X. dendrorhous expression cassette that directs expression by the endogenous glyceraldehyde-3-phosphate dehydrogenase gene expression signals. X. dendrorhous transformation vector pPR2TN was used to deliver this expression cassette, by means of electroporation, into the ribosomal DNA of X. dendrorhous strain CBS 6938, which was confirmed by Southern analysis. High-performance liquid chromatography analysis of the carotenoids produced by idi-overexpressing transformant strains indicated a lower overall carotenoid formation when compared to the control strain. This finding is in contrast to that observed in bacterial systems, where enhanced expression of the idi gene increases the carotenoid content.
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References
Johnson, E. A. and An, G.-H. (1991) Astaxanthin from microbial sources. Crit. Rev. Biotechnol. 11, 297–326.
Sandmann, G. and Misawa, N. (2002) Fungal carotenoids, in The Mycota X Industrial Applications (Osiewacz, H. D., ed.), Springer-Verlag, Berlin, pp. 247–262.
Cerdá-Olmedo, E. (1989) Production of carotenoids with fungi, in Biotechnology of Vitamins, Pigments and Growth Factors (Vandamme, E. J., ed.), Elsevier Applied Science, London, pp. 27–42.
Ernst, H. (2002) Recent advances in industrial carotenoid synthesis. Pure Appl. Chem. 74, 2213–2226.
Kajiwara, S., Fraser, P. D., Kondo, K., and Misawa, N. (1997) Expression of an exogenous isopentenyl diphosphate isomerase gene enhances isoprenoid biosynthesis in Escherichia coli. Biochem. J. 324,421–426.
Verdoes, J. C. and van Ooyen A. J. J. (1999) Isolation of the isopentenyl diphosphate isomerase encoding gene of Phaffia rhodozyma; improved carotenoid production in Escherichia coli. Acta Bot Gallica 146, 43–53.
Wery, J., Verdoes, J. C., and van Ooyen, A. J. J. (1998) Efficient transformation of the astaxanthin-producing yeast Phaffia rhodozyma. Biotechnol. Tech. 12, 399–405.
Verdoes, J. C., Wery, J., Boekhout, T., and van Ooyen, A. J. J. (1997) Molecular characterization of the glyceraldehyde-3-phosphate dehydrogenase gene of Phaffia rhodozyma. Yeast 13, 1231–1242.
Verdoes, J. C., Krubasik, P., Sandmann, G., and van Ooyen, A. J. J. (1999) Isolation and functional characterisation of a novel type of carotenoid biosynthetic gene from Xanthophyllomyces dendrorhous. Mol. Gen. Genet. 262, 453–461.
Andrews, A. G., Phaff, H. J., and Starr, M. (1976) Carotenoids of Phaffia rhodozyma, a red-pigmented yeast. Phy to chemistry 15, 1003–1007.
Britton, G. (1995) Structure and properties of carotenoids in relation to function. FASEB J. 9, 1551–1558.
Andrews, A. G. and Starr, M. (1976) (3R,3′R)-Astaxanthin from the yeast Phaffia rhodozyma. Phy to chemistry 15, 1009–1011.
Sedmak, J. J., Weerasinghe, D. K., and Jolly, S. O. (1990) Extraction and quantitation of astaxanthin from Phaffia rhodozyma. Biotechnol. Tech. 4, 107–112.
Sander, L. C., Sharpless, K. E., Craft, N. E., and Wise, S. A. (1994) Development of engineered stationary phases for the separation of carotenoid isomers. Anal. Chem. 66, 1667–1674.
Maoka, T., Komori, T., and Matsuno, T. (1985) Direct diastereomic resolution of carotenoids I. 3-hydroxy-4-oxo-β-end group. J. Chromatogr. 318, 122–124.
Sambrook, J., Fritsch, E. E, and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Wentzell, L. M., Nobbs, T. J., and Halford, S. E. (1995) The SfiI restriction endonuclease makes a four-stranded DNA break at two copies of its recognition sequence. J. Mol. Biol. 248, 581–595.
Wery, J., Gutker, D., Renniers, A. C. H. M., Verdoes, J. C., and van Ooyen, A. J. J. (1997) High copy number integration into the ribosomal DNA of the yeast Phaffia rhodozyma. Gene 184, 89–97.
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Visser, H., Sandmann, G., Verdoes, J.C. (2005). Xanthophylls in Fungi. In: Barredo, JL. (eds) Microbial Processes and Products. Methods in Biotechnology, vol 18. Humana Press. https://doi.org/10.1385/1-59259-847-1:257
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DOI: https://doi.org/10.1385/1-59259-847-1:257
Publisher Name: Humana Press
Print ISBN: 978-1-58829-548-4
Online ISBN: 978-1-59259-847-2
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