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Isolation and Characterization of Human Airway Fibroblasts in Culture

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Asthma

Abstract

Asthma is considered an airway inflammatory disorder characterized by variable airflow obstruction and airway hyperresponsiveness (1). The inflammatory component of asthma has been studied extensively over the past few years, but, more recently, the potential contribution of airway wall remodeling to functional and clinical changes has been emphasized (2,3). Although the methods of sampling of bronchial tissue were previously limited, being obtained mostly from autopsic or surgical specimens, they have improved recently.

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References

  1. Lask, B. (1993) International consensus report on diagnosis and treatment of asthma. Clin. Exp. Allergy 23, 155–157.

    Article  CAS  PubMed  Google Scholar 

  2. Bousquet, J., Chanez, P., Lacoste, J. Y., Barnéon, G., Ghavanian, N., Enander, I., et al. (1990) Eosinophilic inflammation in asthma. N. Engl. J. Med. 323, 1033–1039.

    Article  CAS  PubMed  Google Scholar 

  3. Boulet, L. P., Chakir, J., Dubé, J., Laprise, C., Boutet, M., and Laviolette, M. (1998) Airway inflammation and structural changes in airway hyper-responsiveness and asthma: an overview. Can. Respir. J. 5, 16–21.

    CAS  PubMed  Google Scholar 

  4. Jeffery, P. K., Wardlaw, A. J., Nelson, F. C., Collins, J. V., and Kay A. B. (1989) Bronchial biopsies in asthma. An ultrastructural, quantitative study and correlation with hyperreactivity. Am. Rev. Respir. Dis. 140, 1745–1753.

    CAS  PubMed  Google Scholar 

  5. Djukanovic, R., Roche, W. R., Wilson, J. W., Beasley, C. R. W., Twentyman, O. P., Howarth, P. H., and Holgate, S. T. (1990) Mucosal inflammation in asthma. Am. Rev. Respir. Dis. 142, 434–457.

    CAS  PubMed  Google Scholar 

  6. Boulet, L. P., Laviolette, M., Turcotte, H., Milot, J., Cartier, A., Dugas, M., Malo, J. L., and Boutet, M. (1997) Bronchial subepithelial fibrosis correlates with airway responsiveness to methacholine. Chest 112, 45–52.

    Article  CAS  PubMed  Google Scholar 

  7. Chakir, J., Laviolette, M., Boutet, M., Laliberté, R., Dubé, J., and Boulet, L. P. (1996) Lower airways remodeling in nonasthmatic subjects with allergic rhinitis. Lab. Invest. 75, 735–744.

    CAS  PubMed  Google Scholar 

  8. Goulet, F., Boulet, L. P., Chakir, J., Tremblay, N., Dubé, J., Laviolette, M., et al. (1996) Morphologic and functional properties of bronchial cells isolated from normal and asthmatic subjects. Am. J. Respir. Cell Mol. Biol. 15, 312–318.

    CAS  PubMed  Google Scholar 

  9. Roche, W. R. (1991) Fibroblasts and asthma. Clin. Exp. Allergy 5, 56–58.

    Google Scholar 

  10. Brewster, C. E., Howarth, P., Djukanovic, R., Wilson, J., and Holgate, S. T. (1990) Myofibroblasts and subepithelial fibrosis in bronchial asthma. Am. J. Respir. Cell Mol. Biol. 3, 507–511.

    CAS  PubMed  Google Scholar 

  11. Gauldie, J., Jordana, M., and Cox, G. (1993) Cytokines and pulmonary fibrosis. Thorax 48, 931–935.

    Article  CAS  PubMed  Google Scholar 

  12. Sime, P. J., Tremblay, G. M., Xing, Z., Sarnstrand, B. O., and Gauldie, J. (1997) Interstitial and bronchial fibroblasts, in Asthma (Barnes, P. J., Grunstein, M. M., Leff, A. R., and Woolcock, A. J., eds.), Lippincott-Raven, Philadelphia, pp. 475–489.

    Google Scholar 

  13. American Thoracic Society Board of Directors (1987) Standards for the diagnosis of patients with COPD and asthma. Am. Rev. Respir. Dis. 136, 225–244.

    Google Scholar 

  14. Anonymous (1992) Investigative use of bronchoscopy, lavage and bronchial biopsies in asthma and other airways diseases. Eur. Respir. J. 5, 115–121.

    Google Scholar 

  15. Anonymous (1991) Workshop summary and guidelines: investigative use of bronchoscopy, lavage, and bronchial biopsies in asthma and other airway diseases. J. Allergy Clin. Immunol. 88, 808–814.

    Google Scholar 

  16. American Thoracic Society (1995) Standardization of spirometry, 1994 update. Am. J. Respir. Crit. Care Med. 152, 1107–1136.

    Google Scholar 

  17. Juniper, E., Cockcroft, D. W., and Hargreave, F. E. (1992) Histamine and metha-choline inhalation tests: tidal breathing method. Laboratory procedure and standardization. Canadian Thoracic Society. AB Draco, Lund, Sweden.

    Google Scholar 

  18. Laviolette, M., Carreau, M., and Coulombe, R. (1988) Bronchoalveolar lavage cell differentialtial on microscope glass cover. Am. Rev. Respir. Dis. 138, 451–457.

    CAS  PubMed  Google Scholar 

  19. Saalbach, A., Anderegg, U., Bruns, M., Schnabel, E., Herrmann, K., and Haustein, U. F. (1996) Novel fibroblast-specific monoclonal antibodies: properties and specificities. J. Invest. Dermatol. 106, 1314–1319.

    Article  CAS  PubMed  Google Scholar 

  20. Dubé, J., Chakir, J., Laviolette, M., Saint-Martin, S., Boutet, M., Desrochers, C., Auger, F., and Boulet, L. P. (1998) In vitro procollagen synthesis and prolifera-tive phenotype of bronchial fibroblasts from normal and asthmatic subjects. Lab. Invest. 78, 297–307.

    PubMed  Google Scholar 

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Chakir, J. et al. (2000). Isolation and Characterization of Human Airway Fibroblasts in Culture. In: Fan Chung, K., Adcock, I. (eds) Asthma. Methods in Molecular Medicine™, vol 44. Humana Press. https://doi.org/10.1385/1-59259-072-1:53

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  • DOI: https://doi.org/10.1385/1-59259-072-1:53

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-626-0

  • Online ISBN: 978-1-59259-072-8

  • eBook Packages: Springer Protocols

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