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Development of Patient-Derived Gastric Cancer Organoids from Endoscopic Biopsies and Surgical Tissues

  • Translational Research and Biomarkers
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Annals of Surgical Oncology Aims and scope Submit manuscript

Abstract

Background

Organoids are three-dimensional in vitro models of human disease developed from benign and malignant gastrointestinal tissues with tremendous potential for personalized medicine applications. We sought to determine whether gastric cancer patient-derived organoids (PDOs) could be safely established from endoscopic biopsies for rapid drug screening.

Methods

Patients underwent esophagogastroduodenoscopy (EGD) for surveillance or staging and had additional forceps biopsies taken for PDO creation. Cancer tissues from operative specimens were also used to create PDOs. To address potential tumor heterogeneity, we performed low-coverage whole-genome sequencing of endoscopic-derived PDOs with paired surgical PDOs and whole-tumor lysates. The stability of genomic alterations in endoscopic organoids was assessed by next-generation sequencing and nested polymerase chain reaction (PCR) assay. The feasibility and potential accuracy of drug sensitivity screening with endoscopic-derived PDOs were also evaluated.

Results

Gastric cancer PDOs (n = 15) were successfully established from EGD forceps biopsies (n = 8) and surgical tissues (n = 7) from five patients with gastric adenocarcinoma. Low-coverage whole-genomic profiling of paired EGD and surgical PDOs along with whole-tumor lysates demonstrated absence of tumor heterogeneity. Nested PCR assay identified similar KRAS alterations in primary tumor and paired organoids. Drug sensitivity testing of endoscopic-derived PDOs displayed standard dose–response curves to current gastric cancer cytotoxic therapies.

Conclusions

Our study results demonstrate the feasibility of developing gastric cancer PDOs from EGD biopsies. These results also indicate that endoscopic-derived PDOs are accurate surrogates of the primary tumor and have the potential for drug sensitivity screening and personalized medicine applications.

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Acknowledgment

We would like to thank Drs. Hans Clevers and Georg Busslinger at the Hubrecht Institute for their instruction on developing organoids. These studies were presented in part at 2018 SSO Annual Symposium.

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Authors and Affiliations

  1. Department of Surgery, Stony Brook University Hospital, New York, USA

    Mei Gao PhD, Miranda Lin BS, Hannah Thompson MD, Kelsi Hirai MD, Georgios V. Georgakis MD, PhD, Aaron R. Sasson MD & Joseph Kim MD

  2. Department of Pathology, Stony Brook University Hospital, New York, USA

    Mei Gao PhD, Manisha Rao MS, James Davis MD, Kenneth R. Shroyer MD, PhD, Jinyu Li PhD & Scott Powers PhD

  3. Division of Medical Oncology, Stony Brook University Hospital, New York, USA

    Minsig Choi MD

  4. Division of Gastroenterology and Hepatology, Stony Brook University Hospital, New York, USA

    Juan Carlos Bucobo MD, Demetri Tzimas MD, Lionel S. D’Souza MD & Jonathan M. Buscaglia MD

Authors
  1. Mei Gao PhD
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  2. Miranda Lin BS
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  3. Manisha Rao MS
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  4. Hannah Thompson MD
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  5. Kelsi Hirai MD
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  6. Minsig Choi MD
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  7. Georgios V. Georgakis MD, PhD
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  8. Aaron R. Sasson MD
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  9. Juan Carlos Bucobo MD
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  10. Demetri Tzimas MD
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  11. Lionel S. D’Souza MD
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  12. Jonathan M. Buscaglia MD
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  13. James Davis MD
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  14. Kenneth R. Shroyer MD, PhD
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  15. Jinyu Li PhD
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  17. Joseph Kim MD
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Corresponding author

Correspondence to Joseph Kim MD.

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None of the authors has a conflict of interest to disclose.

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Gao, M., Lin, M., Rao, M. et al. Development of Patient-Derived Gastric Cancer Organoids from Endoscopic Biopsies and Surgical Tissues. Ann Surg Oncol 25, 2767–2775 (2018). https://doi.org/10.1245/s10434-018-6662-8

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  • DOI: https://doi.org/10.1245/s10434-018-6662-8

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