Abstract
Background
Asymptomatic carriage of infected red blood cells (iRBCs) can be prevalent in communities regardless of transmission patterns and can occur with infection of different Plasmodium species. Clinical immunity dampens the inflammatory responses leading to disease symptoms in malaria. The aim of this study was to define the immunological correlates of asymptomatic carriage of Plasmodium falciparum in a highly exposed population.
Methods
142 asymptomatic Plasmodium-infected individuals greater than 2 years of age without fever (body temperature <37.5 ℃) were followed weekly for 10 weeks before being treated with artemisinin-based combination therapy (ACT). Plasma levels of 38 cytokines were measured at baseline by Luminex and the quantity and growth inhibitory activities of circulating parasite-reactive antibodies measured. The Plasmodium antigen tested included P. falciparum merozoite extract (ME) and schizont extract (SE), and the recombinant proteins erythrocyte binding antigen 175 (EBA-175) and merozoite surface protein 1 (MSP-119).
Results
Median levels of IgG against P. falciparum EBA-175 and MSP-119 at baseline were significantly higher in those older than 20 years of age compared with the younger age group and appeared to correlate with better parasite control. Amongst all participants there were no discernible changes in IgG levels over time. Parasite density was higher in the younger age group and associated with IL-10, TNF and MCP-1 levels. A balanced IL-10:TNF ratio was associated with asymptomatic malaria regardless of age, and balanced ratios of IL-10/TNF and IL-10/IFN-γ were the only significant correlate of maintenance of asymptomatic malaria over the course of the study in individuals 20 years of age and younger.
Conclusion
The above findings indicate that asymptomatic carriage of P. falciparum in children living in a hyperendemic area occurs independently of IgG but is associated with a balanced inflammatory cytokine ratio.
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Background
Malaria is still a significant global health problem with an estimated 249 million cases and 608 000 deaths in 2022 [1]. Although mortality rates from malaria have decreased considerably since the turn of the century [2]. this has been paralleled by high increases in proportions of asymptomatic infections, known to contribute significantly to persistent malaria transmission in most endemic countries [3, 4]. Asymptomatic infections can persist for weeks to years [5] and untreated P. falciparum infections have been shown persist on average for 6 months, with fluctuations in parasite density over the course of an infection [6, 7]. The maintenance of chronic asymptomatic malaria will depend on the host immune response to the blood stage of Plasmodium [4]. Anti-parasite immunity directly suppress parasite growth resulting in low infection densities (below the pyrogenic threshold [4, 8]) whilst anti-disease immunity modulates inflammation-mediated clinical manifestations of the infection.
Older children and adults have more cumulative exposure to parasite variants circulating within the community, developing adaptive responses which mediate both anti-parasite and anti-disease immunity [8, 9]. However, asymptomatic infections have been documented in younger children < 5 years who are too young to have developed robust adaptive immunity [10]. This suggests that asymptomatic malaria may be differentially controlled by innate and adaptive immune responses depending on age.
Anti-parasite immunity which is acquired with age or exposure [11, 12], is mediated mainly by antibodies which control parasite replication and mediate clearance of Plasmodium-infected red blood cells (iRBCs) [13,14,15]. Suppression of inflammation associated with iRBCs depends on the balance between anti- and pro-inflammatory cytokine responses [16, 17] and it has been hypothesized that asymptomatic infections are associated with a shift in host immune responses towards an anti-inflammatory profile after multiple exposures to malaria [18]. This could occur via a decrease in levels of pro-inflammatory immune mediators such as IFN-γ, TNF, or an increase in anti-inflammatory mediators such as IL-10 or TGF-β [17, 19,20,21].
The relative contributions of anti-parasite and anti-disease immunity to the maintenance of persistent asymptomatic Plasmodium infections in younger individuals and older individuals remain unclear. To determine the immunological associations of chronic asymptomatic malaria in younger (≤ 20 years) and older (> 20 years) individuals a longitudinal study of highly exposed individuals of all ages with asymptomatic malaria over a 3-month period was carried out where the stability of IgG and cytokine responses was monitored in all participants. These responses were also quantified upon clearance of parasites to ascertain which responses depended on the presence of circulating parasites. This study demonstrated a lack of correlation of iRBC density and IgG responses in individuals ≤ 20 years old, with an association of parasite carriage with IL-10 and an association of asymptomatic status with a balanced ratio of IL-10:TNF and IL-10: IFN-γ.
Methods
Ethics statement
The study protocol was approved by the Cameroon National Ethics Committee for Human Health Research (Ethical clearance N°: 2018/09/1104/CE/CNERSH/SP) and the Ministry of Public Health. Written informed consent was obtained from each participant or parent/guardian of minor participants (< 19 years old) prior to enrolment into the study. Assent was obtained from all children older than 12 years of age.
Study area and population
The study population consisted of individuals of both genders aged at least 2 years of age and permanently living in one of the five selected villages of the Esse health district, Mefou-et-Afamba Division, Centre Region of Cameroon where malaria transmission is perennial, stable and hyper-endemic with P. falciparum as the predominant Plasmodium species. The study area has been described elsewhere [22,23,24]. The inclusion criteria were: (i) obtain the consent of each older participant or parent or legal guardian for the children, (ii) living permanently in the study area, (iii) not pregnant.
Study design and participants
This was a longitudinal household-based study that involved 353 individuals. Participants were considered asymptomatic for malaria if positive for P. falciparum using multiplex nested PCR in the absence of fever at least forty-eight hours prior to enrolment. Being febrile was assessed based on axillary temperature ≥ 37.5 ℃ or based on the history of fever as independently confirmed by another household member. All symptomatic individuals (fever + rapid diagnostic test (RDT) +) were treated with ACT-based drugs following recommended national guidelines. Participants with asymptomatic carriage of Plasmodium parasites were monitored weekly until they either developed a fever, or until the end of the follow up period at week 16 post-enrolment. Participant follow-up comprised of weekly interviews regarding the occurrence of fever during the past week, measurement of axillary temperatures on each visit, and laboratory testing for malaria at weeks 1, 4, 11 and 16 (PCR and microscopy). All symptomatic cases occurring during the follow-up period were treated with ACT as recommended and excluded from further follow-up. At week 11 post-enrolment all participants with persisting asymptomatic parasitaemia were treated as recommended, and monitored for five additional weeks to assess the effect of ACT-based anti-malarial treatment on selected host immune responses. Participants with persistent asymptomatic parasitaemia 10 weeks following enrolment were considered as persistent asymptomatic malaria whereas those who developed malaria-associated symptoms before week 11 post-enrolment were considered as developing uncomplicated malaria.
Blood sample collection, malaria diagnosis and parasite genotyping
Approximately 3 ml of venous blood was collected in EDTA-coated tubes and used for laboratory testing for malaria and blood hemoglobin levels as well as to obtain plasma for immunological analysis. Plasma was separated from the red blood cell (RBC) pellet by centrifugation (3500 RPM, 5 min) and stored at − 80 ℃ until use. Blood haemoglobin levels were assessed using a portable Mission Hb haemoglobinometer (ACON Laboratories, USA).
Parasite density (parasites/µl) was determined by microscopic examination of Giemsa-stained thick blood smears. Parasite density was determined based on the number of parasites per at least 200 WBC count on a thick film, assuming a total white blood cell count of 8000 cells/µl of whole blood. A blood slide was considered positive when a concordant result was obtained by at least two independent microscopists and when a discrepancy occurred, a third microscopist was used for confirmation. A slide was considered negative if no parasites were detected after a count of 500 white blood cells.
Genomic DNA was extracted from dried blood spots using the Chelex method [25], and used to screen for Plasmodium infections by multiplex nested PCR targeting the 18 S small subunit rRNA (ssrRNA) genes as previously described [24]. Genotyping of msp2 gene was performed by nested PCR on P. falciparum PCR positive samples as previously described [24, 26].
Case definitions for the study
Participants were defined as asymptomatic if positive for malaria parasites by PCR in the absence of fever at least 48 h prior to enrolment. Febrile malaria was determined based on RDT, light microscopy or PCR positivity for malaria parasites and axillary temperature ≥ 37.5 ℃ or a history of fever in the past 48 h. Those developing uncomplicated malaria were defined as asymptomatic individuals who had developed fever associated with Plasmodium infection during the 10-week infection follow-up period, whereas the persistent asymptomatic malaria classification represented the asymptomatic individuals who remained asymptomatic until anti-malarial treatment at the end of the infection follow-up period.
Anti-Plasmodium antibody levels and avidity assessment
Plasma IgG antibody levels and avidity were determined by indirect ELISA method using P. falciparum Merozoite extract (ME), schizont extract (SE) or the recombinant proteins erythrocyte binding antigen-175 (EBA-175) and Merozoite Surface Protein-119 (MSP119) as coating antigens. ME and SE were isolated from laboratory maintained P. falciparum 3D7 parasites as described previously [27], and used for protein extraction by freeze-thaw fractionation [23, 28]. The recombinant protein EBA-175 (F3 region) was obtained from BEI Resources (MRA-1162), whereas P. falciparum MSP119 was produced using a baculovirus-insect cell expression system [29].
96-well microplates (F96 CERT-Maxisorp) were coated with each antigen (2 mg/ml of ME and SE, 0.20 mg/ml of EBA-175 and 0.5 mg/ml of MSP-119) in 0.1 M bicarbonate buffer (for ME, SE and EBA-175) or PBS (for MSP-119) and incubated overnight at 4 ℃. Plasma samples were diluted (1/250) in PBS-T/1%BSA and used to measure anti-Plasmodium IgG as previously described [23, 30]. Plasma from non-exposed European blood donors (10 samples) were used as naive controls and a pool of IgG fractions from adults living in high malaria endemic areas was used as positive controls. Results were expressed as optical-density ratios (OD sample/mean OD naive control). For avidity testing, 100 µl of 8 M urea in wash buffer (treated wells) or 100 µl of wash buffer (untreated wells) were added for 15 min at room temperature to the respective wells following primary antibody binding as previously described [31]. Avidity indices (AI) were calculated as proportion of bound antibodies following urea treatment (AI = [mean OD of treated wells /mean OD of untreated wells] × 100). Samples with OD ratios > 2, corresponding to mean OD naive control + 3SD were considered as seropositive. In addition, only seropositive samples were used for avidity testing.
Growth inhibition assay (GIA)
GIA was performed using plasma from endemic and non-endemic individuals as described by Duncan et al [32]. Briefly, plasma samples were heat inactivated at 56 ℃ for 20 min and a 1:20 ratio of 50% RBC was added to each sample and incubated at room temperature for 1 h to remove the RBC-reactive antibody. After spinning for 2 min at 13,000 × g to pellet the RBCs, the supernatant was transferred to a 96-well V-bottom plate. Synchronized late trophozoite/early schizont (30–40 h) stages of 3D7 P. falciparum at 0.5% parasitaemia were incubated with plasma (1:10 ratio) for 48 h at 37 ℃. The GIA harvest (parasite growth) was carried out by SYBR green-I DNA quantification assay [33]. Parasite growth inhibition (GI) activities of plasma antibodies were presented as: GI indices (%) = 100 x [1 - ((OD sample - OD RBC) / (OD parasitized RBC - OD RBC))].
Determination of plasma cytokine levels
Plasma concentrations of 38 immune mediators including interleukin (IL)-1α, IL-1β, IL-1RA, IL-2 IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p40, IL-p70, IL-13, IL-15, IL-17 A, interferon (IFN)-α2, IFN-γ, tumour necrosis factor (TNF), lymphotoxin-α (LT-α), IFN-γ induced protein (IP-10), monocyte chemoattractant protein (MCP)-1, MCP-3, macrophage inflammatory protein (MIP)-1α, MIP-1β, Eotaxin, Fractalkine, human growth-regulated oncogene (GRO), Macrophage-derived chemokine (MDC), granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), sCD40L, FMS-like tyrosine kinase 3 ligand (Flt-3 L), epidermal growth factor (EGF), fibroblast growth factor 2 (FGF-2), transforming growth factor alpha (TGF-α) and vascular endothelial growth factor (VEGF) were determined by Luminex-based method using the Human Cytokine/Chemokine Magnetic Bead Panel MILLIPLEX MAP Kit (Cat. No. HCYTMAG-60 K-PX38, EMD Millipore Corporation) according to the manufacturer’s instructions. Briefly, 25 µl of premixed magnetic capture beads were incubated with equal volumes of plasma or diluted standard in a 96-well microplate placed on a horizontal plate shaker for 2 h. After washing using a magnetic plate, plates were incubated with 25 µL/well of biotinylated detection antibodies for 1 h at room temperature followed by 25 µl of streptavidin-PE in each well for 30 min at room temperature. Median fluorescence intensity was read on a Luminex MAGPIX Analyser (XMAP Technology) equipped with xPONENT software version 4.2. The relative concentration of each cytokine (pg/ml) in each sample was determined based on the automatically generated standard curve for each analyte. Analyte amounts less than the limit of detection for each cytokine were attributed the value of detection limit.
Statistical analysis
Statistical analyses were performed using RStudio version 4.1.1 or version 4.2.2 [34] and Graphpad Prism version 8.4.3 (Graphpad software, San Diego, California USA). Differences in antibody and cytokine distribution between two or more groups were determined using Wilcoxon signed rank test and Kruskal-Wallis test, respectively. Spearman rank correlation tests or linear regression were used to determine the correlation between quantitative variables. Differences in categorical variables were determined by chi-square tests. P-values < 0.05 were considered statistically significant. Factor analysis of mixed data (FAMD) with FactoMineR and factoextra packages was used to assess the association between cytokines levels and age and circulating parasite loads. The complexHeatmap and ggpubr packages were used to draw the heatmaps and the line plots, respectively. Given that the aim of this study was to determine the host immunological determinants in the maintenance of the asymptomatic infections, among the 353 individuals included, only participants with complete information on the study outcome during the longitudinal follow-up period were included in the analysis (n = 211). Participants who missed at least one of the follow-up points (the predominant reason being farming far away from the enrolment site) were not included in the analysis. Three samples (2 symptomatic and 1 asymptomatic) were excluded because no cytokines were detected in the plasma. For analysis of cytokines at the initial time point of the study with respect to correlation with persistent asymptomatic malaria, a mixed linear model was applied to the data using the LME4 package [35]. Cytokine level was used as the response with conversion status as the fixed-effect of the model. Sex and age were included as random effects in the model and ran the model separately for children ≤20 years old, adults >20 years old and all participants, regardless of age. To obtain P-value estimates from the t-values the lmerTest package was used [36]. P values were corrected for multiple comparisons using the Sattherthwaite correction. For analysis, parasite densities were separated into three groups as follows: (1). submicroscopic parasitaemia, (2). low levels of microscopic parasitaemia (parasitaemia between 37.2 parasites/µl and 413.1 parasites/µl) and (3). high levels of parasitaemia (parasitaemia > 413.1 parasites/µl, representing the median parasitaemia in the study population).
Results
Asymptomatic malaria prevalence in the study area
Immunological analysis of a subset of 211 individuals from a previously reported study of 353 individuals screened for malaria using PCR was undertaken, where 328 (92.9%) were positive for P. falciparum infection, of which 266 (81.1%) were asymptomatic and 62 (18.9%) were symptomatic [24]. This subset had complete sampling from the follow-up study, and included 142 individuals with asymptomatic infection, 61 symptomatic individuals and 8 non-infected individuals. At baseline when these individuals were first sampled, parasite densities were highly variable between the symptomatic and asymptomatic groups, and no significant difference was observed in the median parasite densities of the two groups at the initial time point of the study (Table 1). Age, sex, and haemoglobin levels were also not different across the study groups at this initial time point (Table 1).
Anti-parasite antibodies were inversely correlated with parasitaemia but were not associated with asymptomatic parasite carriage
Consistent with the hypothesis that host immunity increases with repeated exposure to malaria parasites, baseline parasite densities above the detection limit of microscopy decreased significantly with age (r = − 0.49, P < 0.0001) (Fig. 1A) but only in individuals aged 20 years and younger (r= − 0.39, P < 0.0001) (Fig. 1B). Of the 142 participants who completed the follow-up study as designed, 78 (54.9%) remained asymptomatic during the 10-week follow-up period, whereas 63 (44.4%) developed malaria-associated fevers and 1 (0.7%) resolved the infection in the absence of any known anti-malarial treatment. Based on the parasitaemia distribution of those with microscopic Plasmodium infections at baseline the study population was divided into two groups aged ≤ 20 years and > 20 years old for further analyses. The proportion of submicroscopic infections was significantly higher (P < 0.0001) in the older age group (age > 20 years) at the baseline time point than in the younger age group (Fig. 1C). At the baseline time point, median IgG levels against P. falciparum merozoite (ME) and schizont (SE) crude extracts were significantly higher in the > 20-year age group than in the younger age group (P < 0.0001) (Fig. 2A). This was mirrored by IgG reactive to P. falciparum recombinant proteins EBA-175 (P = 0.0250) and MSP-119 (P < 0.0001) (Fig. 2A). Additionally, in the younger age group IgG levels against merozoite (r = 0.22, P = 0.0077) and schizont (r = 0.29, P = 0.0004) crude extracts were positively correlated with age at the baseline time point (Fig. 2B). This trend was not observed with anti-MSP-119 IgG (r = 0.15, P = 0.0764) or anti-EBA-175 IgG (r = 0.01, P = 0.8793). A negative correlation was observed between IgG levels to P. falciparum merozoite and schizont extracts at baseline and parasite density measured at the same time point (Supplementary Fig. 1). Indeed, the multilevel linear regression model adjusted for village and household levels, using IgG levels at the baseline and age as predictors, showed that only age was the independent predictor of Plasmodium parasite densities in the study population (coef = − 0.02, P = 0.0004 for age; coef = 0.01, P = 0.9170 for ME; coef= − 0.19, P = 0.1529 for SE; coef= − 0.02, P = 0.4746 for EBA-175; coef = 0.02, P = 0.5362 for MSP-119).
Older individuals (> 20 years) had a better control of parasite densities. A Scatterplot showing a correlation between baseline parasite densities and age in all the infected individuals with parasitaemia at the detection limit of light microscopy (n = 138). Spearman rank correlation is presented as the best fit line and the coefficient (r), as well as the P-value (p), are shown. The red straight line was used to divide the study population into two groups aged ≤ 20 years and > 20 years old for further analyses. B Scatterplot of correlation between baseline parasitaemia detected by microscopy and age in different age groups (≤ 20 years and > 20 years) at the different time points of this study. Spearman rank correlation is presented as the best fit line and the coefficient (r), as well as the P-value (p), are shown for each age group. C The pie chart shows the proportion of sub-microscopic infections (PCR+/microscopy-) versus microscopic (microscopy+) infections in each age group. PCR: Polymerase Chain Reaction
Individuals > 20 years old had more anti-parasite IgG at baseline. A Data presented in the scatterplot represent baseline IgG antibody levels against different P. falciparum antigens (ME, SE, EBA-175 and MSP-119) within age groups. Median levels between age groups were compared by Wilcoxon signed-rank test and the P-values are shown on the graph. B The scatterplots on B show the correlation between IgG levels against different P. falciparum antigens (ME, SE, EBA-175 and MSP-119) and age in different age groups (≤ 20 years and > 20 years). Spearman rank correlation is presented as the best fit line and the coefficient (r), as well as the P-values (p), are shown for each age group. SE: Schizont extract. ME: Merozoite extract. EBA-175: Erythrocyte binding antigen-175. MSP-119: Merozoite surface protein-119. OD: optical density
Median IgG levels were not significantly different between participants with sub-microscopic parasitaemia and those with microscopic parasites at baseline (Supplementary Fig. 2A), and increased in vitro growth inhibition potential of inactivated plasma from submicroscopic parasite carriers was greater than for microscopic carriers ≤20 years old but not for those > 20 years old (Supplementary Fig. 2B). Both IgG antibody avidity (Supplementary Fig. 3A, B) and growth inhibition potentials (Supplementary Fig. 2B) were similar in the two groups (P > 0.05). Together, these findings suggest that IgG may not be the main mediator that controls parasite density levels and that the plasma may contain other agents that can exert direct inhibition of parasite growth in vivo such as IgM.
Similarly, no significant difference was observed in malarial IgG antibody levels between participants with symptomatic or asymptomatic parasitaemia (Supplementary Fig. 4A), and no significant difference was seen in the blood parasitaemia at the detection limit of microscopy (Supplemental Fig. 4B; Table 1). However, the in vitro growth inhibition potential of plasma from asymptomatic malaria was greater than that of symptomatic individuals (P = 0.0245) despite no significant differences in the parasitaemia amongst those with parasite levels that were detectable by microscopy (Supplementary Fig. 4B). This may be a reflection of higher percentage of asymptomatic children with submicroscopic parasitaemias (22.3%) compared to symptomatic children (9.5%) (Supplementary Fig. 4C). Taken together, these data suggest that humoral responses other than IgG responses to the tested parasite antigens contribute to the maintenance of asymptomatic parasitaemia in the study area.
Persistence of fluctuating asymptomatic parasitaemia occurred in highly exposed individuals without alteration in IgG levels
Of the 142 participants who completed the follow-up study as designed, 72 (50.7%) remained asymptomatic during the 10-week follow-up period, whereas 69 (48.6%) developed malaria-associated fevers and 1 (0.7%) resolved the infection in the absence of any known anti-malarial treatment. Surprisingly, the propensity to convert was not associated with age (Fig. 3A). Of the 72 participants who maintained chronic asymptomatic P. falciparum carriage, the proportion of submicroscopic infections remained similar in both age groups with most participants becoming microscopy positive on week 10 regardless of age (Fig. 3B). Parasite densities fluctuated widely over the follow-up period from ultra-low parasitaemia (sub-microscopic) to high parasitaemia (Fig. 3C), possibly due to variation in the infecting clones from new infections as this is a high transmission area (Fig. 3D). However, no significant changes in IgG levels (Fig. 3E) against all antigens tested were observed during persistent asymptomatic infections indicating that persistent asymptomatic parasitaemia does not affect anti-Plasmodium IgG levels over this short 3-month time period. These data suggests that asymptomatic carriage of P. falciparum occurs independently of circulating parasite numbers, even within highly-exposed individuals.
Asymptomatic malaria persisted in some individuals despite changes in the circulating parasite densities and population over time, but did not affect the antibody levels. A The pie charts show the proportion of individuals with initial asymptomatic infection (cross-sectional) who resolved the infection (clearers), developed malaria-associated fever (uncomplicated malaria) or remained asymptomatic (persistent asymptomatic) over the 10-week follow-up period, according to age groups. B Panel B displays the proportion of sub-microscopic/microscopic parasitaemia in persistent asymptomatically infected individuals at three different time points (weeks 1, 4 and 11) according to age groups. Individuals who were RDT positive but without detectable micoroscopic parasitaemia was detected are shown in pink. Of those who had microscopic parasitaemia, the line plots parasitaemia across the 3 time points (C). To generate the line plots, the value 10 was assigned to submicroscopic parasitaemia. The P values on the line plots refer to the comparison of the median parasitaemia between two time points using the Wilcoxon test. D Pie charts show the proportion of msp2 alleles (representing the clones) determined by nested PCR in individuals with persistent asymptomatic infection per week. Numbers represent the band sizes obtained. Blue slices represent the alleles only present at week 1. Red slices represent the alleles which appear at week 4. Green slices represent the allele only present at week 11. Grey slices represent the alleles that were present at all three sampling time points (bands of 375, 400, 475, 500, 520, 534, 540, 600, 620, 633. 680, 700, 800 bp). E Data presented in the line plots represent the profile of IgG anti-Plasmodium antibody levels in each chronic asymptomatically infected individuals at three different time points (week 1, 4 and 11) in the two age groups. SE: Schizont extract. ME: Merozoite extract. EBA-175: Erythrocyte binding antigen-175. MSP-119: Merozoite surface protein-119. OD: optical density
Parasite density was associated with inflammatory responses amongst younger individuals with asymptomatic malaria
Given that Plasmodium parasites are associated with induction of inflammatory responses the cytokine profiles of the 72 participants who had persistent asymptomatic carriage of P. falciparum across the course of the study was determined. There was no particular cytokine signature that associated with asymptomatic carriage of P. falciparum in the study participants over the 11-week follow-up period ( Fig. 4A). Using a factor analysis of mixed data (FAMD) on cytokine data along with age and circulating parasite density, the levels of IL-10, TNF, the ratio of IL-10:IFN-γ and MCP-1, along with age and parasite density explained most of the variation in the peripheral cytokine response in asymptomatic individuals (Fig. 4B). Multivariate analysis using age and parasitaemia as predictors showed that age independently predicted the levels of TNF (coef = −0.31, P < 0.0001) peaking in younger study participants (Fig. 4C). This is likely a reflection of circulating parasite densities which were higher in the younger group (Fig. 1) and were also independently predictive of TNF levels (coef = 0.09, P < 0.0001) (Fig. 4D).
Cytokine responses were blunted with increased age and correlated with parasite densities at baseline. A Hierarchical clustering of the standardized least-square means of 38 differentially expressed cytokines/chemokines/growth factors across the three sampling time points in participants with persistent asymptomatic infection (n = 72). B Principal component of cytokines, age and parasitaemia from FAMD (left graph) analysis. The right graph of B shows the contribution of each variable on the principal component 2 (dimension 2). C and D box plots representing interquartile ranges (25th, median and 75th percentile, whisker percentile (1st and 99th)) of the levels (Log10 pg/ml) of some main regulatory cytokines measured by Luminex according to age and parasite level groups at the baseline, respectively. Median levels of cytokines between two or more groups were compared by Wilcoxon rank-sum test and Kruskal-Wallis test, respectively. In Panel A, pairwise comparisons on neighboring groups were performed only when multiple comparisons were significant at p < 0.05. Parasite densities were groups in three groups as follows: submicroscopic parasitaemia, low levels of microscopic parasitaemia (parasitaemia ≤ 413.1 parasites/µl, representing the median parasitaemia in the study population) and high levels of parasitaemia (parasitaemia > 413.1 parasites/µl). FAMD: Factor analysis of mixed data. *p < 0.05; **p < 0.001, ***p < 0.0001, ****p < 0.00001
Circulating parasite density also independently predicted the levels of IL-10 (coef = 0.21, P < 0.0001) and MCP-1 (coef = 0.06, P = 0.01) with a significant increase in those who had high levels of parasitaemia (parasitaemia > 413.1 parasites/µl) relative to those who had low levels of microscopic parasitaemia (parasitaemia between 37.2 parasites/µl and 413.1 parasites/µl) (Fig. 4D). This indicates that during asymptomatic carriage of P. falciparum circulating parasites simultaneously induce anti-inflammatory cytokine production to counteract the inflammatory effects of TNF-α. To determine which cytokines were dependent on parasite carriage participants were treated at week 11 of the study with ACT. This resulted in a significant decline in the IL-10 levels, but not TNF, in the younger age group (Fig. 5). Thus, the levels of IL-10 measured is a reflection of parasite carriage in the younger age group.
IL-10 and TNF-α levels were a reflection of parasite carriage, and the balance of inflammatory cytokines at baseline was associated with the maintenance of asymptomatic infections in the younger age, ≤ 20 years. A Box plots representing interquartile ranges (25th, median and 75th percentile, whisker percentile (1st and 99th)) of cytokine levels (Log10 pg/ml) during persistent asymptomatic infections (week 1, 4 and 11) and after anti-malarial treatment (week 16). B Box plots representing interquartile ranges (25th, median and 75th percentile, whisker percentile (1st and 99th)) of cytokine levels (Log10 pg/ml) at the baseline between in those who developed uncomplicated malaria (UM; grey) and those who went on to display persistent asymptomatic malaria throughout the course of the study (PAM; blue). Analyses were performed including values from individuals with submicroscopic and microscopic parasitaemia. Median cytokines levels between two or more groups were compared by Wilcoxon rank-sum test and Kruskal-Wallis test, respectively and significant between group differences indicated by the P value on the plot. In Panel A, pairwise comparisons on neighboring groups were performed only when multiple comparisons between the 4 groups were significant and are shown by the grey brackets. ***p < 0.0001
Maintenance of asymptomatic carriage of P. falciparum is associated with a more balanced inflammatory cytokine ratio
There was no particular cytokine signature of asymptomatic individuals relative to symptomatic individuals in this study (Fig. 6A). However, given that anti-inflammatory cytokines may be important in down-regulating the pathogenic inflammatory response in malaria, the hypothesis that asymptomatic infection would be associated with higher levels of IL-10 was tested. Surprisingly, at the initial time point of the study there was a non-significant trend towards higher levels of IL-10 in the symptomatic compared to asymptomatic participants (Fig. 6B). This may be a reflection of higher inflammation levels in symptomatic individuals as other pro-inflammatory markers (IL-6 and MCP-1) were significantly higher in individuals with symptomatic malaria compared to those with asymptomatic P. falciparum infections in the younger age group (Fig. 6B). In agreement with previously published data in individuals with uncomplicated malaria, there was an IL-10-skewed ratio of IL-10:TNF and IL-10:IFN-γ in younger individuals (Fig. 6C). An IL-10 skewed ratio of IL-10 to TNF-α has also previously been measured in those with severe symptoms of malaria [37, 38]. Multivariable logistic regression analysis showed that the IL-10:TNF ratio and IL-1RA levels were independently associated with developing symptoms during P. falciparum infection in this study (Supplementary Table S1).
IL-10 at baseline was associated with clinical malaria in the younger age group (≤ 20 years). A Hierarchical clustering (heatmap) of the standardized least-square means of 38 differentially expressed cytokines/chemokines/growth factors by infection status at the baseline of this study. B and C box plots represent interquartile ranges (25th, median and 75th percentile, whisker percentile (1st and 99th)) of the baseline cytokine levels (Log10 pg/ml) measured by Luminex according to infection status. Analyses were performed including both individuals submicroscopic and microscopic parasitaemia. Median levels of cytokines between two or three groups were compared by Wilcoxon rank-sum test and Kruskal-Wallis test, respectively. In Panel B and C, pairwise comparisons were performed only when multiple comparisons were significant. P values refer to the Kruskal-Wallis test comparison between the three groups. NI: Non-infected. AS: Asymptomatic. SY: Symptomatic. *p < 0.05; **p < 0.001; ***p < 0.0001
To further test the hypothesis that asymptomatic carriage of P. falciparum may be related to IL-10 production, the cytokine ratios in the younger group who converted to symptomatic malaria throughout the study was compared to those who remained asymptomatic. At the initial time point of the study there was a trend towards increased IL-10 levels in those who subsequently went on to develop uncomplicated malaria compared to those who experienced persistent asymptomatic malaria in both age groups. In the younger age group IL-10/pro-inflammatory cytokine ratios were significantly higher and IL-10-skewed (P = 0.0207 and P = 0.0111 for IL-10:TNF and IL-10:IFN-γ ratios, respectively) in the those developing uncomplicated malaria compared to those with persistent asymptomatic malaria in younger age group (Fig. 5B). In this group those with persistent asymptomatic malaria had a ratios of 1.001±0.1355 for IL-10:TNF and 1.016±0.1794 for IL-10:IFN-γ) displaying an even balance of IL-10: proinflammatory cytokine. On the other hand adults did not have IL-10 skewed ratios with TNF or IFN-γ suggesting that a different or additional mechanism may facilitate the persistence of asymptomatic malaria in the older age group. Collectively this data suggests that IL-10 is not sufficient to dampen inflammatory responses in Plasmodium infection that lead to the fevers that define uncomplicated malaria.
Discussion
Significant gaps exist in the understanding of the contribution of anti-parasite and anti-disease immunity in the persistent asymptomatic parasite carriage in areas with high transmission of P. falciparum. Mathematical modelling of existing data indicates that asymptomatic P. falciparum infections could last for as long as 13-years [5] and field data documents the longevity of asymptomatic infections ranges from at least 2–9 months [7, 39, 40]. As previously published, Esse has high rate of persistent asymptomatic parasite carriage over the 3 month time period of study [24]. However, a proportion of individuals in this study converted to symptomatic malaria as defined by the development of fever. Thus, immune correlates of asymptomatic carriage of P. falciparum was assessed, as well as the predictors of conversion to symptomatic malaria.
Consistent with the acquisition of immune response with age and exposure [12, 41] in this study, older asymptomatic individuals had better control of circulating iRBCs with a greater proportion of sub-microscopic infections than younger individuals. This was associated with an increase in the quantity of circulating anti-parasite IgG which appeared to saturate at around 20 years old. This data is in agreement with an age-structured mathematical model of malaria transmission which showed that anti-parasite immunity which results in more rapid clearance of parasitaemia has an approximate half-life of 20 years [42]. With a focus on the younger age group, there did not appear to be any correlations with respect to parasitaemia levels and IgG levels or the avidity of the IgG. The in vitro invasion-blocking capability of the plasma in young individuals had some functionality with respect to invasion blocking, but only at higher parasitaemia levels. In this assay plasma factors other than IgG, such as IgM [43, 44], may contribute to this observation and control of P. falciparum parasitaemia in children. Collectively these data support a role for IgG in exerting control of iRBCs in P. falciparum infections, but it may not be the principal factor that dictates asymptomatic carriage of parasites in younger individuals.
Surprisingly the proportion of those developing uncomplicated malaria over the course of this study was comparable between the younger and the older age groups, suggesting that the conversion from an asymptomatic to symptomatic infection is independent of age in highly exposed individuals. The reasons for this finding are not clear but may be related to heterogeneity of the circulating P. falciparum parasite population and emergence of highly inflammatory parasite clonotypes [45, 46] that overcome the pyrogenic threshold of individuals. Consistent with the oscillation of parasite densities over time reported in a Vietnam cohort [7], parasitaemia in persistent asymptomatic infections in this study fluctuated widely and independently of IgG levels, and different clonotypes of P. falciparum were observed at different time points in this study.
Cytokine profiles are considerably more dynamic than IgG responses. In line with the hypothesis that clinical immunity to malaria is mediated by a tempering of inflammation, previous studies have shown that the clinical outcome of malaria infection is largely dictated by the balance between pro-inflammatory and anti-inflammatory markers [16, 17]. Although this study highlighted that carriage of P. falciparum across the duration of this study was associated with a consistent inflammatory response characterized by TNF, IL-10 and MCP-1, no obvious cytokine signature associated with the asymptomatic carriage of P. falciparum was found when compared to symptomatic individuals. Similar to findings in a study of visceral leishmaniasis [47], where increased IL-10 levels were the primary predictor of clinical conversion from asymptomatic to symptomatic visceral leishmaniasis, the conversion from asymptomatic to symptomatic malaria in this study was linked to increased baseline levels of IL-10 skewed ratios with TNF and IFN-γ in children at the initial time point of this study.
Although IL-10 is known to exert anti-inflammatory effects that include inhibition of monocytes and macrophages in the production of inflammatory cytokines such as IL-6, TNF, and IL-l [48], in agreement with a recent study from The Gambia [49] in this study IL-10 was associated with the presence of Plasmodium parasites. Given that higher levels of IL-6, IL-1RA and MCP-1 were also associated with symptomatic infections in the younger age group of this study, IL-10 levels are likely a consequence of increased inflammation. Notably, IL-10 induces IL1RA, a potent anti-inflammatory cytokine that regulates the pro-inflammatory actions of IL-1 [50] that has previously been shown to be associated with severity of malarial disease [51]. At the baseline time point in this study, it was found that balanced inflammatory cytokine ratios were correlated with protection against developing symptoms of P. falciparum infection in the younger age group. Specifically, those who developed uncomplicated malaria had a significant IL-10 skewed ratio at the initial time point compared to those who had persistent asymptomatic malaria. An IL-10 skewed ratio may indicate that an individual will shortly develop symptoms of P. falciparum infection.
Conclusion
Most of the association between biomarkers and clinical malaria in this study were only found in the younger age group, but not in adults, indicating that the mechanisms of the protection against clinical malaria may be different according to age. As such, more studies into how asymptomatic carriage of Plasmodium occurs are warranted and should include non-antibody mediated mechanisms of parasite control and clinical immunity which may be relevant in children.
Data availability
Data are available from the authors upon request and with permission from the co-corresponding authors.
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Acknowledgements
We thank the field staff and, most importantly, residents of the Esse Health district in Cameroon who participated in this study. This information has previously been presented at the American Association for Tropical Medicine and Hygiene Annual Meeting in 2019.
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This work was supported by the Institute Pasteur International Division to LSA.
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BF devised the experiments, performed the experiments, analyzed the data, and wrote the paper; FM, MFA, EE, CD, GC, MK, RK and SK performed fieldwork and sample processing; MS and RP provided cytokine kits and recombinant P. falciparum antigens, respectively and edited the manuscript. RM supervised the experiments and edited the paper; LA and TL devised the experiments, supervised the experiments, analyzed data, and wrote the paper.
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Fogang, B., Schoenhals, M., Maloba, F.M. et al. Asymptomatic carriage of Plasmodium falciparum in children living in a hyperendemic area occurs independently of IgG responses but is associated with a balanced inflammatory cytokine ratio. Malar J 23, 268 (2024). https://doi.org/10.1186/s12936-024-05086-8
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DOI: https://doi.org/10.1186/s12936-024-05086-8