Abstract
The goal of the work was to study the age-related variability in the fibroblast proliferative activity in the paravasal connective tissue of the hollow and parenchymal organs derived from male corpses of the first mature period, elderly age, and senile age. The proliferation index was based on the expression of the Ki-67 protein (MIB-1 clone). It was found that the proliferation index in the first mature period was similar in the paravasal tissue cells of the parenchymal and hollow organs of the digestive tract. At the same time, the fibroblasts of heart paravasal tissue had the lowest proliferation activity of all the studied organs. The intensity of cell proliferation decreased with age; moreover, distinct organ specificity was revealed over the age of 75 years. The proliferation index in paravasal tissue cells of the parenchymal organs was the lowest of all the studied organs. The paravasal-tissue cells of the hollow organs retained significantly higher proliferative activity in the senile age; it was almost two times higher than that in parenchymal organs derived from the same age group.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Ventsel’, E.S., Teoriya veroyatnostei: Uchebnik dlya studentov vysshikh tekhnicheskikh uchebnykh zavedenii (The Theory of Probability: Manual for Students of Higher Engineering Institutions), Moscow: Yustitsiya, 2018.
Volkov, V.P., New approaches to heart organometry, Sovrem. Med.: Aktual. Vopr., 2013, no. 22, pp. 29–39.
Dobrynina, I.V., Tel’tsov, L.P., and Muzyka, I.G., Polysaccharides of the connective tissue cells of the wall of the duodenum of human embryos, Vestn. Nov. Med. Tekhnol., 2014, no. 1, p. 138.
Zhuklina, V.V., Gorbunov, N.S., and Samotesov, P.A., Anatomical features of the anterior abdominal wall in the elderly: a literature review, Sib. Med. Obozr., 2012, no. 6, pp. 39–43.
Ivina, A.A., Semkin, V.A., and Babichenko, I.I., KI-67 and GLUT1 proteins in diagnostics of neoplasia of the oral mucosa, Stomatologiya, 2016, no. 6–2, pp. 11–12.
Kondrat’eva, E.V., Adil’gereeva, E.P., Amelina, E.L., et al., Obtaining induced pluripotent stem cells from fibroblasts of a patient with cystic fibrosis, Sib. Med. Obozr., 2019, no. 2, pp. 95–101.
Maiborodin, I.V., Morozov, V.V., Matveeva, V.A., et al., Morphological changes during use of bone marrow multipotent stromal cells to recover the lymph flow in the thrombosed vein area, Morfologiya, 2015, no. 5, pp. 48–55.
Maiborodin, I.V., Shevela, A.I., Morozov, V.V., et al., Specific tissue reaction after experimental ligation of a limb vein, Flebologiya, 2017, no. 3, pp. 154–163.
Mel’nikova, O.V., Morphofunctional characteristics of rat spleen in experimental hypercalcemia, Vestn. Ivanovsk. Med. Akad., 2016, no. 2, pp. 20–24.
Nikitina, T.D., Volkov, A.V., and Emshanova, Yu.P., Clinical and anatomical aspects of the morphofunctional state of the vascular–paravasal tissue complex, Materialy X Vsesoyuznogo s”ezda anatomov, gistologov i embriologov (Proc. X All-Union Congr. of Anatomists, Histologists, and Embryologists), Moscow, 1986, p. 251.
Rasulova, Kh.A., The morphological status of the kidneys in experimental ischemic stroke, Sib. Med. Obozr., 2017, no. 1, pp. 54–59.
Khismatullina, Z.N., Biochemical changes in connective tissue during aging and other pathological processes, Vestn. Kazan. Tekhnol. Univ., 2012, no. 8, pp. 237–243.
Chen, J.I., Ahn, T.I., Colón-Bernal, D.I., et al., The relationship of collagen structural and compositional heterogeneity to tissue mechanical properties: a chemical perspective, ACS Nano, 2017, vol. 11, pp. 10665–10671.
Lago, J.C. and Puzzi, M.B., The effect of aging in primary human dermal fibroblasts, PLoS One, 2019, vol. 14, art. ID 0219165.
Mavroudis, P.D., DuBois, D.C., Almon, R.R., and Jusko, W.J., Modeling circadian variability of core-clock and clock-controlled genes in four tissues of the rat, PLoS One, 2018, vol. 13, art. ID 0 197 534.
Wang, Y.I., Rouabhia, M., Lavertu, D., and Zhang, Z., Pulsed electrical stimulation modulates fibroblasts’ behavior through the Smad signaling pathway, J. Tissue Eng. Regener. Med., 2017, vol. 11, pp. 1110–1121.
Woodley, D.T., Distinct fibroblasts in the papillary and reticular dermis: implications for wound healing, Dermatol. Clin., 2017, vol. 35, pp. 95–100.
Zhang, S., Wang, L., and Zan, L., Investigation into the underlying molecular mechanisms of white adipose tissue through comparative transcriptome analysis of multiple tissues, Mol. Med. Rep., 2019, vol. 19, pp. 959–966.
ACKNOWLEDGMENTS
The authors are grateful to Prof. S.Yu. Nikulina, the rector of the Voyno-Yasenetsky Krasnoyarsk State Medical University for the support of this study. All experiments were carried out with the use of the facility of the Krasnoyarsk State Medical University.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interests. The authors declare that they have no conflicts of interest.
Statement on the welfare of animals. This article does not contain any studies involving animals performed by any of the authors.
Additional information
Translated by A. Boutanaev
Rights and permissions
About this article
Cite this article
Nickel, V.V., Efremova, V.P. Proliferative Activity of Cells of Paravasal Connective Tissue at Different Ages. Adv Gerontol 10, 162–165 (2020). https://doi.org/10.1134/S2079057020020125
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S2079057020020125